A5003626205- Mechanical Circulatory Support Devices
- Cardiac Ischemia and Reperfusion
- Fuel Cells and Related Materials
- Transplantation: Methods and Outcomes
- Extracellular vesicles in disease
- Organ Transplantation Techniques and Outcomes
- Cardiac Structural Anomalies and Repair
- Cervical and Thoracic Myelopathy
- Cardiac Arrest and Resuscitation
- Renal Transplantation Outcomes and Treatments
- Osteoarthritis Treatment and Mechanisms
- Spine and Intervertebral Disc Pathology
- Renin-Angiotensin System Studies
- Orthopedic Surgery and Rehabilitation
- TGF-β signaling in diseases
- Rheumatoid Arthritis Research and Therapies
- Mitochondrial Function and Pathology
University Hospital of Bern
2021-2025
University of Bern
2021-2025
The advent of normothermic, ex-situ heart perfusion (ESHP) enables new options for evaluation cardiac grafts. Donation after circulatory death (DCD) is a promising solution to improve transplantation rates, but current ESHP approaches are recognized as suboptimal DCD We aimed develop vascular function test graft assessment during using porcine model DCD. Explanted hearts were perfused unloaded 3 hours followed by left ventricular loading 1 hour measure recovery. During perfusion, hypercapnic...
Cardiac donation after circulatory death is a promising option to increase graft availability. Graft preservation with 30 minutes of hypothermic oxygenated perfusion (HOPE) before normothermic machine may improve cardiac recovery as compared cold static storage, the current clinical standard. We investigated role preserved nitric oxide synthase activity during HOPE on its beneficial effects.
Abstract OBJECTIVES Donation after circulatory death provides excellent patient outcomes in heart transplantation; however, warm ischaemic graft damage remains a concern. We have reported that brief period of hypothermic oxygenated perfusion prior to normothermic reperfusion improves recovery rat model. Here we investigated the cardioprotective benefits and mechanisms this approach compared current clinical standard large animal METHODS Circulatory was induced anesthetized Male Schweizer...
Background During donation after circulatory death (DCD), cardiac grafts are exposed to potentially damaging conditions that can impact their quality and post-transplantation outcomes. In a clinical DCD setting, patients have closed chests in most cases, while many experimental models used open-chest conditions. We therefore aimed investigate characterize differences open- vs. closed-chest porcine models. Methods Withdrawal of life-sustaining therapy (WLST) was simulated anesthetized...
Conditions to which the cardiac graft is exposed during transplantation with donation after circulatory death (DCD) can trigger recruitment of macrophages that are either unpolarized (M0) or pro-inflammatory (M1) as well release extracellular vesicles (EV). We aimed characterize effects M0 and M1 macrophage-derived EV administration on post-ischaemic functional recovery glucose metabolism using an isolated rat heart model DCD. Isolated hearts were subjected 20 min aerobic perfusion, followed...
Introduction: Donation after circulatory death (DCD) could substantially improve donor heart availability. In DCD, the is not only exposed to a period of warm ischemia, but also damaging pre-ischemic phase. We hypothesized that DCD-relevant lactate levels negatively affect post-ischemic functional and mitochondrial recovery in an isolated rat model DCD. Methods: Isolated, working hearts underwent 28.5′ global ischemia 60′ reperfusion. Prior were perfused with one three levels: no (0 Lac),...