A5079993874- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Inflammatory Bowel Disease
- Microbial Metabolic Engineering and Bioproduction
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Clinical Nutrition and Gastroenterology
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- Cancer Immunotherapy and Biomarkers
- Advanced Proteomics Techniques and Applications
- IL-33, ST2, and ILC Pathways
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Protein Degradation and Inhibitors
- Mesenchymal stem cell research
- Biotin and Related Studies
- Bacteriophages and microbial interactions
- Neurobiology and Insect Physiology Research
- Genomics and Phylogenetic Studies
Boston Children's Hospital
2021-2025
Broad Institute
2021-2025
Massachusetts Institute of Technology
2021-2025
Ragon Institute of MGH, MIT and Harvard
2021-2025
Science for Life Laboratory
2020-2023
Karolinska Institutet
2020-2023
Harvard University
2022
KTH Royal Institute of Technology
2021
University of Notre Dame
2015
Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive their environment. Of all cellular macromolecules, bacterial proteome is by far most important resource its size limited. Here, we investigated how highly versatile 'knallgas' bacterium Cupriavidus necator reallocates protein resources when grown on limiting substrates with rates. We determined quantity mass spectrometry estimated enzyme utilization analysis modeling....
Tissue-specific T cell immune responses play a critical role in maintaining organ health but can also drive pathology during both autoimmunity and alloimmunity. The mechanisms controlling intratissue programming remain unclear. Here, we leveraged nonhuman primate model of acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem transplantation to probe the biological underpinnings tissue-specific alloimmune using comprehensive systems immunology approach including...
Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs allows for genetically encoded, site-specific incorporation of non-canonical amino acids (ncAAs) as chemical handles protein labeling and modification. Here, we demonstrate that piggyBac-mediated genomic integration archaeal pyrrolysine tRNA (tRNA
Genetically encoded fluorescent tags for visualization of proteins in living cells add six to several hundred amino acids the protein interest. While suitable most proteins, common easily match and exceed size microproteins 60 or less. The added molecular weight structure such tag may thus significantly affect vivo biophysical biochemical properties microproteins. Here, we develop single-residue terminal labeling (STELLA) that introduce a single noncanonical acid either at N- C-terminus...
Crohn’s disease is an inflammatory bowel (IBD) commonly treated through anti-TNF blockade. However, most patients still relapse and inevitably progress. Comprehensive single-cell RNA-sequencing (scRNA-seq) atlases have largely sampled with established treatment-refractory IBD, limiting our understanding of which cell types, subsets, states at diagnosis anticipate severity response to treatment. Here, combining clinical, flow cytometry, histology, scRNA-seq methods, we profile diagnostic...
During the larval stages, visual system of mosquito Aedes aegypti contains five stemmata, often referred to as ocelli, positioned laterally on each side head. The stemmata contain two photoreceptor types, distinguished by expression different rhodopsins. Aaop3 rhodopsin is expressed in majority photoreceptors. There are small clusters photoreceptors located within satellite and central that express Aaop7 instead Aaop3. Electroretinogram analysis transgenic Drosophila indicates Aaop7, both...
Crohn’s disease is an inflammatory bowel (IBD) commonly treated through anti-TNF blockade. However, most patients still relapse and inevitably progress. Comprehensive single-cell RNA-sequencing (scRNA-seq) atlases have largely sampled with established treatment-refractory IBD, limiting our understanding of which cell types, subsets, states at diagnosis anticipate severity response to treatment. Here, combining clinical, flow cytometry, histology, scRNA-seq methods, we profile diagnostic...
Abstract SARS-CoV-2 infection and COVID-19 disease vary with respect to viral variant host vaccination status. However, how vaccines, emergent variants, their intersection shift responses in the human nasal mucosa remains uncharacterized. We others have shown during first wave that a muted epithelial interferon response at site of underlies severe COVID-19. sought further understand upper airway cell subsets states associate phenotypes across variants vaccination. Here, we integrated new...
Abstract Crohn’s disease is an inflammatory bowel (IBD) commonly treated through anti-TNF blockade. However, most patients still relapse and inevitably progress. Comprehensive single-cell RNA-sequencing (scRNA-seq) atlases have largely sampled with established treatment-refractory IBD, limiting our understanding of which cell types, subsets, states at diagnosis anticipate severity response to treatment. Here, combining clinical, flow cytometry, histology, scRNA-seq methods, we profile...
Summary Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive their environment. Of all cellular macromolecules, bacterial proteome is by far most important resource its size limited. Here, we investigated how highly versatile ‘knallgas’ bacterium Cupriavidus necator reallocates protein resources when grown on limiting substrates with rates. We determined quantity mass spectrometry estimated enzyme utilization analysis...
ABSTRACT Genetic code expansion via stop codon suppression is a powerful strategy to engineer proteins. Pyrrolysyine-tRNA (tRNA Pyl )/pyrrolysyl-tRNA synthetase (PylRS) pairs from methanogenic archaea and engineered bacterial tRNA/aminoacyl-tRNA synthetases (aaRS) are used for site-specific incorporation of noncanonical amino acids (ncAAs) in response codons mammalian cells. Routinely, ncAA achieved by transient expression the tRNA/aaRS pair leading heterogeneous suppression. Genomic...
Genetic code expansion via stop codon suppression is a powerful strategy to engineer proteins. Suppressor tRNAs are aminoacylated with noncanonical amino acids (ncAAs) by dedicated aminoacyl-tRNA synthetases (aaRS) and direct site-specific ncAA incorporation during translation. These pairs of tRNA/aaRS must be orthogonal the host’s tRNAs, aaRS natural acids. Pyrrolysyl-tRNA (PylT)/PylRS from methanogenic archaea, as well engineered derived bacteria, used for genetic in mammalian cells. Amber...