A5074904720- HIV/AIDS drug development and treatment
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- HIV Research and Treatment
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Pneumonia and Respiratory Infections
- Syphilis Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Immune responses and vaccinations
- Single-cell and spatial transcriptomics
- Prenatal Screening and Diagnostics
- Clinical Laboratory Practices and Quality Control
- Health and Medical Research Impacts
- Biotin and Related Studies
- Streptococcal Infections and Treatments
- Vitamin D Research Studies
- Infectious Diseases and Tuberculosis
- Reproductive tract infections research
- Leprosy Research and Treatment
- Pharmaceutical studies and practices
- LGBTQ Health, Identity, and Policy
- Sexuality, Behavior, and Technology
- Antibiotic Use and Resistance
- SARS-CoV-2 detection and testing
Jackson Memorial Hospital
2022-2025
University of Mississippi Medical Center
2021-2025
The University of Texas Southwestern Medical Center
2017-2020
Parkland Health & Hospital System
2020
Albert Einstein College of Medicine
2010-2019
Southwestern Medical Center
2018
Parkland Memorial Hospital
2017
Columbia University Irving Medical Center
2015
Yeshiva University
2010-2012
For transgender individuals taking hormone therapy (HT), data on laboratory values are limited, and the effects cannot be easily predicted. We evaluated impact common analytes in before after initiation of HT.We conducted a retrospective chart review patients identified at transgender-specific clinics an urban county hospital community clinic. Laboratory were collected concentrations, hematologic parameters, electrolytes, lipids, liver renal markers 183 women (TW) 119 men (TM) for whom...
Pneumococcal pneumonia continues to be a significant global health burden, affecting both children and adults. Traditional diagnostic methods for sputum analysis remain challenging. The objective of this study was twofold: develop rapid easy-to-perform assay the identification Streptococcus pneumoniae (Spn) directly in specimens using fluorescence microscopy, characterize with high-resolution confocal microscopy ultrastructure pneumococci residing human sputum. We fluorescently labeled...
To investigate biotin interference on three cardiac troponin (cTn) assays and demonstrate a method to overcome interference.cTn levels were measured in (1) plasma from healthy volunteers 10-mg daily supplementation mixed with known elevated troponin, (2) plasmas cTn after mixing reagent simulate supplementation, (3) biotin-spiked specimens pretreated streptavidin-agarose beads.Daily ingestion (10 mg) studies simulating use resulted significant the Gen5 T (cTnT) assay; contemporary Gen 4 cTnT...
Malaria, caused by Plasmodia parasites, affects hundreds of millions people. As purine auxotrophs, use transporters to import host purines for subsequent metabolism the salvage pathway. Thus are attractive drug targets. All sequenced genomes encode four ENTs (equilibrative nucleoside transporters). During pathogenic intraerythrocytic stages, ENT1 is a major route nucleoside/nucleobase transport. Another plasma membrane transporter exists because Plasmodium falciparum ENT1-knockout parasites...
Equilibrative transporters are potential drug targets; however, most functional assays involve radioactive substrate uptake that is unsuitable for high-throughput screens (HTS). We developed a robust yeast-based growth assay potentially applicable to many equilibrative transporters. As proof of principle, we applied our approach Nucleoside Transporter 1 the malarial parasite Plasmodium falciparum (PfENT1). PfENT1 inhibitors might serve as novel antimalarial drugs since PfENT1-mediated purine...
Purine transport is essential for malaria parasites to grow because they lack the enzymes necessary de novo purine biosynthesis. The Plasmodium falciparum Equilibrative Nucleoside Transporter 1 (PfENT1) a member of equilibrative nucleoside transporter (ENT) gene family. PfENT1 primary pathway across P. plasma membrane knock-out are not viable at physiologic extracellular concentrations. Topology predictions and experimental data indicate that ENT family members have eleven transmembrane (TM)...
Infection with Plasmodium falciparum and vivax cause most cases of malaria. Emerging resistance to current antimalarial medications makes new drug development imperative. Ideally a should treat both Because malaria parasites are purine auxotrophic, they rely on purines imported from the host erythrocyte via Equilibrative Nucleoside Transporters (ENTs). Thus, import transporters represent potential target for development. For primary transporter is P. Transporter Type 1 (PfENT1). Recently we...
Detection of SARS-CoV-2 viral RNA is important for the diagnosis and management COVID-19.We present a clinical validation reverse transcription PCR (RT-PCR) assay nucleocapsid (N1) gene. Off-board lysis on an automated nucleic acid extraction system was optimized with endemic coronaviruses (OC43 NL63). Genomic in recombinant protein coat were used as control materials compared recovery from extraction.Nucleic showed decreased Coronavirus vitro transcribed (NL63) attenuated virus (OC43). had...
Abstract We observed a higher rate of blood-culture contamination during the COVID-19 pandemic at our institution compared to prepandemic period. Given potential implications blood in antibiotic and diagnostic test utilization as well added cost, it is imperative continue efforts minimize these episodes pandemic.
Emerging resistance to current antimalarial medicines underscores the importance of identifying new drug targets and novel compounds. Malaria parasites are purine auxotrophic import purines via Plasmodium falciparum equilibrative nucleoside transporter type 1 (PfENT1). We previously showed that PfENT1 inhibitors block parasite proliferation in culture. Our goal was identify additional, possibly more optimal chemical starting points for a discovery campaign. performed high throughput screen...
Abstract Objective We evaluate diagnostic accuracy of the ARCHITECT chemiluminescent immunoassay (CIA) screening test in pregnancy, and pregnancy outcomes among screen-positive women. Study Design Samples from routine prenatal rapid plasma reagin (RPR) tests were collected between June 22 August 18, 2017 frozen. batch-tested with Abbott syphilis TP (CIA, index test). calculated sensitivity, specificity, predictive value, false positivity. compared neonatal Results Of 1,602 specimens, 35...
A 36-year-old man presented to a haematology clinic with jaundice, headache, shortness of breath, fatigue and erectile dysfunction. Nine years earlier, he had been diagnosed hereditary spherocytosis, haemoglobin concentration (Hb) between 60 70 g/l. At that time, painful splenomegaly was treated by splenectomy, his Hb increasing above 120 He stable clinical course during the interim years; however, given current symptoms drop in 87 g/l, bone marrow biopsy performed. The peripheral blood film...
Abstract SARS-CoV-2 infection and COVID-19 disease vary with respect to viral variant host vaccination status. However, how vaccines, emergent variants, their intersection shift responses in the human nasal mucosa remains uncharacterized. We others have shown during first wave that a muted epithelial interferon response at site of underlies severe COVID-19. sought further understand upper airway cell subsets states associate phenotypes across variants vaccination. Here, we integrated new...
Background: Pneumococcal pneumonia continues to be a significant global health burden, affecting both children and adults. Traditional diagnostic methods for sputum analysis remain challenging. The objective of this study was twofold: develop rapid easy-to-perform assay the identification Streptococcus pneumoniae (Spn) directly in specimens using fluorescence microscopy, characterize with high-resolution confocal microscopy ultrastructure pneumococci residing human sputum. Methods: We...