A5026197830- Cystic Fibrosis Research Advances
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Gut microbiota and health
- Urinary Tract Infections Management
- Clostridium difficile and Clostridium perfringens research
- Biosensors and Analytical Detection
- Urological Disorders and Treatments
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Antifungal resistance and susceptibility
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Pediatric Urology and Nephrology Studies
- Vibrio bacteria research studies
- Viral Infections and Outbreaks Research
- Tracheal and airway disorders
- Neonatal and Maternal Infections
- Fungal Infections and Studies
The University of Texas Southwestern Medical Center
2019-2025
Southwestern Medical Center
2021-2022
Dartmouth College
2012-2019
University of Utah
2017
Rochester General Hospital
2012
Rochester Institute of Technology
2008
The airways of patients with cystic fibrosis are colonized diverse bacterial communities that change dynamically during pediatric years and early adulthood. Staphylococcus aureus is the most prevalent pathogen childhood, but late teens adulthood, a shift in microbial composition occurs leading to Pseudomonas aeruginosa community predominance ∼50% adults. We developed robust dual-bacterial vitro coculture system P. S. on monolayers human bronchial epithelial cells homozygous for ΔF508...
Diverse microbial communities chronically colonize the lungs of cystic fibrosis patients. Pyrosequencing amplicons for hypervariable regions in 16S rRNA gene generated taxonomic profiles bacterial sputum genomic DNA samples from 22 patients during a state clinical stability (outpatients) and 13 acute exacerbation (inpatients). We employed quantitative PCR (qPCR) to confirm detection Pseudomonas aeruginosa Streptococcus by pyrosequencing data human oral microbe identification microarray...
Human-associated microbial communities include fungi, but we understand little about which fungal species are present, their relative and absolute abundances, how antimicrobial therapy impacts communities. The disease cystic fibrosis (CF) often involves chronic airway colonization by bacteria these infections cause irreversible lung damage. Fungi detected more frequently in CF sputum samples upon initiation of therapy, several studies have implicated the detection fungi with worse outcomes....
The second messenger cyclic diguanylate (c-di-GMP) plays a critical role in the regulation of motility. In Pseudomonas aeruginosa PA14, c-di-GMP inversely controls biofilm formation and surface swarming motility, with high levels this dinucleotide signal stimulating repressing swarming. P. encodes two stator complexes, MotAB MotCD, that participate function its single polar flagellum. Here we show repression motility requires functional complex. Mutating motAB genes restores to strain...
Abstract Background Metagenomic next-generation sequencing (mNGS) for pathogen detection offers the potential broad directly from clinical specimens. However, yield and impact of testing is variable, financial cost high, questions surrounding its optimal use remain. Our pediatric institution used a committee-based approach to discuss approve or deny mNGS test requests. In this study, we evaluate patient characteristics which was considered, yield, results when employing model diagnostic...
Abstract Background Clostridioides difficile testing by polymerase chain reaction (PCR) does not differentiate asymptomatic colonization from infection, and children are frequently colonized with toxigenic strains of C. difficile. Over-diagnosis infections can lead to adverse patient outcomes poorer quality metrics for hospitals.Figure 1:C. PCR orders following change in GI Panel, Interrupted Time Series Methods In January 2023 our pediatric health system enacted multimodal changes the...
Cystic fibrosis (CF) is a genetic disease that causes patients to accumulate thick, dehydrated mucus in the lung and develop chronic, polymicrobial infections due reduced mucociliary clearance. These chronic subsequent decline function are significant factors morbidity mortality of CF.
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants continue to emerge, and effective tracking requires rapid return of results. Surveillance is typically performed by whole genome sequencing (WGS), which can be financially prohibitive specialized equipment bioinformatic expertise. Genotyping approaches are methods for monitoring SARS-CoV-2 but require continuous adaptation. Fragment analysis may represent an approach improved variant detection. Methods...
Here we demonstrate the use of an RNA-based analysis specific taxa interest, including bacteria and fungi, within microbial communities. This multiplex method may be useful as a means to identify samples with combinations gain information on how populations vary over time space or in response perturbation. A rapid measure bacterial fungal aid study host changes
Interest continues to grow regarding the role of serologic assays for detection prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted emergency use authorization (EUA) status many SARS-CoV-2 assays. In this document, expert recommendations from clinical microbiologist members American Society Microbiology (ASM) concerning detailed verification strategies FDA EUA are provided, as insights into assay...
Pseudomonas aeruginosa produces outer membrane vesicles (OMVs) that contain a number of secreted bacterial proteins, including phospholipases, alkaline phosphatase, and the CFTR inhibitory factor (Cif). Previously, Cif, an epoxide hydrolase, was shown to be regulated at transcriptional level by epoxides, which serve as ligands repressor, CifR. Here, we tested whether epoxides have effect on Cif levels in OMVs. We showed growth P. presence specific but not hydrolysis product increased...
In the United States, response to COVID-19 coronavirus pandemic has been hampered by inadequate testing resources for causative virus SARS-CoV-2. early part of pandemic, States laboratories were initially heavily regulated and slow provide testing. As progressed, supply chain instruments reagents inconsistent revealed weaknesses in traditional sophisticated infectious disease Testing capabilities clinical could be substantially improved assays that are more simplified do not require multiple...
Detection of SARS-CoV-2 viral RNA is important for the diagnosis and management COVID-19.We present a clinical validation reverse transcription PCR (RT-PCR) assay nucleocapsid (N1) gene. Off-board lysis on an automated nucleic acid extraction system was optimized with endemic coronaviruses (OC43 NL63). Genomic in recombinant protein coat were used as control materials compared recovery from extraction.Nucleic showed decreased Coronavirus vitro transcribed (NL63) attenuated virus (OC43). had...
Abstract In non-endemic settings, transfusion-transmitted malaria (TTM) is rare but potentially fatal and becoming more common with globalization. We present two pediatric cases that demonstrate donor screening using questionnaires subject to error TTM should be considered fever following numerous transfusions in children, particularly sickle cell patients.
Abstract Background Human parechovirus (HPeV) infection can result in severe disease infants, including sepsis, seizures, brain injury, and death. In 2022, a resurgence of HPeV was noted young infants. The spectrum illness outcomes remain to be fully described. Methods A multistate retrospective cohort study conducted evaluate hospitalizations infants aged ≤6 months admitted 2022 with laboratory-confirmed infection. Infants were defined as having clinical or abnormalities on magnetic...