A5024273335- Helicobacter pylori-related gastroenterology studies
- Parasitic Infections and Diagnostics
- Bacterial Identification and Susceptibility Testing
- Parasite Biology and Host Interactions
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
- Parasites and Host Interactions
- Mosquito-borne diseases and control
- Amoebic Infections and Treatments
- Veterinary medicine and infectious diseases
- Viral Infections and Vectors
- Dermatological diseases and infestations
- SARS-CoV-2 detection and testing
- Gastrointestinal disorders and treatments
- SARS-CoV-2 and COVID-19 Research
- Salmonella and Campylobacter epidemiology
- Respiratory viral infections research
- Infective Endocarditis Diagnosis and Management
- Galectins and Cancer Biology
- Parasitic Diseases Research and Treatment
- Gastric Cancer Management and Outcomes
- Cytomegalovirus and herpesvirus research
- Parasitic infections in humans and animals
- Bartonella species infections research
- Antimicrobial Resistance in Staphylococcus
University of Utah
2016-2025
University Pathologists
2011-2025
ARUP Laboratories (United States)
2015-2024
ARUP Institute for Clinical and Experimental Pathology
2013-2022
Genomics (United Kingdom)
2019-2021
Institute for Transfusion Medicine
2019
University of Alberta
2005-2010
ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a relatively new addition to the clinical microbiology laboratory. The performance MALDI Biotyper system (Bruker Daltonics) was compared those phenotypic and genotypic identification methods for 690 routine referred isolates representing 102 genera 225 unique species. We systematically direct-smear extraction on taxonomically diverse collection isolates. optimal score thresholds bacterial...
Standard two-tiered testing (STTT) is the recommended algorithm for laboratory diagnosis of Lyme disease (LD). Several limitations are associated with STTT that include low sensitivity in early stages disease, as well technical complexity and subjectivity second-tier immunoblotting; therefore, modified (MTTT) algorithms utilize two sequential first-tier tests eliminate immunoblotting have been evaluated. Recently, a novel MTTT uses VlsE chemiluminescence immunoassay followed by C6 enzyme has...
Advances in laboratory techniques have revolutionized parasitology diagnostics over the past several decades. Widespread implementation of rapid antigen detection tests has greatly expanded access to for global parasitic threats such as malaria, while next-generation amplification and sequencing methods allow sensitive specific human animal parasites complex specimen matrices.
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid and accurate tool for the identification many microorganisms. We assessed this technology 103 Haemophilus parainfluenzae, Aggregatibacter aphrophilus, actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, Kingella kingae (HACEK) clinical isolates 20 influenzae isolates. Ninety-three percent HACEK organisms were identified correctly to genus level using Bruker database, 100%...
Intestinal protozoa are responsible for relatively few infections in the developed world, but testing volume is disproportionately high. Manual light microscopy of stool remains gold standard can be insensitive, time-consuming, and difficult to maintain competency. Artificial intelligence digital slide scanning show promise revolutionizing clinical parasitology laboratory by augmenting detection parasites interpretation using a convolutional neural network (CNN) model. The goal this study...
Lipopolysaccharide (LPS) is a major component on the surface of Gram negative bacteria and composed lipid A-core O antigen polysaccharide. polysaccharides gastric pathogen Helicobacter pylori contain Lewis antigens, mimicking glycan structures produced by human cells. The interaction antigens with dendritic cells induces modulation immune response, contributing to H. virulence. amount position in LPS varies among isolates, indicating an adaptation host. In contrast most bacteria, genes for...
Leptotrichia spp. are anaerobic, pencil-shaped, Gram-negative rods that part of the normal oral and intestinal human flora. Although not typically considered pathogenic, invasive infections have been reported in immunosuppressed patients. A perceived rise identification at our institution prompted a retrospective evaluation these infections. Laboratory clinical records were reviewed to identify culture-positive Over 5-year period, 68 Leptotrichia-positive specimens identified. Of these, 21%...
INTRODUCTIONIn November 2015, the Centers for Disease Control and Prevention (CDC) sent a letter to state territorial epidemiologists, public health laboratory directors, officials. In this letter, culture-independent diagnostic tests (CIDTs) detection of enteric pathogens were characterized as "a serious current threat surveillance, particularly Shiga toxin-producing Escherichia coli (STEC) Salmonella" The document says CDC its partners are approaching issue, in part, by "reviewing...
Due to the limited sensitivities of stool-based microscopy and/or culture techniques for Strongyloides stercoralis, detection antibodies this intestinal nematode is relied upon as a surrogate determining exposure status or making diagnosis S. stercoralis infection. Here, we evaluated three immunoassays, including recently released InBios Strongy Detect IgG enzyme-linked immunosorbent assay (ELISA) (InBios International, Inc., Seattle, WA), SciMedx serology microwell ELISA (SciMedx...
Background: Rapid multiplex polymerase chain reaction (PCR) assays simultaneously detect several respiratory viral pathogens with high sensitivity. Maximizing detection of influenza at the point care has potential to reduce unnecessary antibiotic use, laboratory tests and hospitalizations. However, cost-effectiveness rapid PCR for not been compared other diagnostic methods in children. Methods: For children presenting emergency department influenza-like illness, we costs outcomes using 4...
Acute Q fever diagnosis via paired serology is problematic because it requires follow-up for convalescent sample collection; as such, cannot provide a to inform treatment decision at the time of acute presentation. Real-time polymerase chain reaction (PCR) may be useful approach in endemic settings. Among febrile patients enrolled sentinel surveillance study two referral hospitals Moshi, Tanzania, from 2012 2014, we analyzed those with sera IgG Coxiella burnetii (C. burnetii) phase II...
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ABSTRACT Development of severe gastric diseases is strongly associated with those strains Helicobacter pylori that contain the cag pathogenicity island (PAI) inserted into chromosome. The PAI encodes a type IV secretion system translocates major disease-associated virulence protein, CagA, host epithelial cell. CagA then affects signaling pathways, leading to cell elongations and inflammation. Since precise mechanism by which toxin translocated remained elusive, we used fusion proteins...
We report three cases of infection due to the Gram-negative rod Ignatzschineria (Schineria) indica involving bacteremia and urinary tract. Two were clearly associated with maggot infestation, third could conceivably have had unrecognized infestation believe these be first I. infections reported in association myiasis.
Background Respiratory pathogens are a leading cause of hospital admission and traditional detection methods time consuming insensitive. Multiplex molecular have recently been investigated in hope replacing these techniques with rapid panel‐based testing. Objectives This study evaluated the F ilm A rray ® R espiratory P anel ([ FARP ], Idaho Technology Inc., Salt Lake City, UT ) as replacement for direct fluorescent antibody ( DFA testing pediatric hospital. Methods Eleven 21 analytes...
Interest continues to grow regarding the role of serologic assays for detection prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted emergency use authorization (EUA) status many SARS-CoV-2 assays. In this document, expert recommendations from clinical microbiologist members American Society Microbiology (ASM) concerning detailed verification strategies FDA EUA are provided, as insights into assay...
Abstract Background As serologic assays for SARS-CoV-2 become more widely utilized, it is important to understand their performance characteristics and correlation with neutralizing antibodies. We evaluated 3 commonly used IgG (Abbott, DiaSorin, EUROIMMUN) clinical sensitivity, specificity, antibodies, then compared antibody kinetics during the acute phase of infection. Methods Three panels samples were tested on every assay. Sensitivity was assessed using a panel 35 specimens serially...