A5042447716- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Streptococcal Infections and Treatments
- HIV/AIDS drug development and treatment
- Infectious Diseases and Mycology
- Fungal Infections and Studies
- HIV Research and Treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- Gut microbiota and health
- Infective Endocarditis Diagnosis and Management
- DNA Repair Mechanisms
- Diphtheria, Corynebacterium, and Tetanus
- Influenza Virus Research Studies
- Genomics and Phylogenetic Studies
- Antifungal resistance and susceptibility
- Microbial infections and disease research
- Vector-borne infectious diseases
- Rabies epidemiology and control
- Clostridium difficile and Clostridium perfringens research
- DNA and Nucleic Acid Chemistry
- Plant Pathogens and Fungal Diseases
- Acne and Rosacea Treatments and Effects
- Actinomycetales infections and treatment
- Cancer therapeutics and mechanisms
University of Utah
2014-2025
ARUP Laboratories (United States)
2014-2025
Translational Research Institute
2020-2024
Queensland University of Technology
2017-2024
University Pathologists
2011-2024
Rutgers New Jersey Medical School
1994-2022
Rutgers, The State University of New Jersey
1994-2022
National Institutes of Health
2005-2020
National Heart Lung and Blood Institute
2020
University of California, San Francisco
2019
ABSTRACT We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed >1.5-fold, including many involved fatty acid metabolism. The most highly induced showed homology with nonribosomal peptide synthetases/polyketide synthases.
Previous studies have shown that a σ 54 –σ S cascade regulates the expression of few key lipoproteins in Borrelia burgdorferi , agent Lyme disease. Here, we demonstrate these sigma factors, both together and independently, regulate much more extensive number genes cellular processes. Microarray analyses mutant strains identified 305 regulated by 145 whereas regulatory appears to control 48 B. . In silico revealed nearly 80% with altered were linked potential -dependent promoters. Many...
Escherichia coli sequence type 131 (ST131), typically fluoroquinolone-resistant (FQ-R) and/or extended-spectrum β-lactamase (ESBL)-producing, has emerged globally. We assessed its prevalence and characteristics among US veterans.In 2011, 595 de-identified E. clinical isolates were collected systematically within 3 resistance groups (FQ-susceptible [FQ-S], FQ-R, ESBL-producing) from 24 nationally distributed Veterans Affairs Medical Centers (VAMCs). ST131 H30 subclone detected by polymerase...
The genus Nocardia has undergone rapid taxonomic expansion in recent years, and an increasing number of species are recognized as human pathogens. Many established have predictable antimicrobial susceptibility profiles, but sufficient information is often not available for recently described organisms. Additionally, the effectiveness sulfonamides first-line drugs been questioned. This led us to review patterns a large molecularly identified clinical isolates. Susceptibility results were...
Shigella species are so closely related to Escherichia coli that routine matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) cannot reliably differentiate them. Biochemical and serological methods typically used distinguish these species; however, "inactive" isolates E. biochemically very similar thus pose a greater diagnostic challenge. We ClinProTools (Bruker Daltonics) software discover MALDI-TOF MS biomarker peaks generate classification models...
Leptotrichia spp. are anaerobic, pencil-shaped, Gram-negative rods that part of the normal oral and intestinal human flora. Although not typically considered pathogenic, invasive infections have been reported in immunosuppressed patients. A perceived rise identification at our institution prompted a retrospective evaluation these infections. Laboratory clinical records were reviewed to identify culture-positive Over 5-year period, 68 Leptotrichia-positive specimens identified. Of these, 21%...
ABSTRACT Background Campylobacter jejuni is a leading pathogen causing gastroenteritis in humans. Invasive C. strains crossing the gut-blood barrier can cause bacteremia. Despite its microaerophilicity (preferring <5% O 2 atmosphere), hyper-aerotolerant (HAT) has recently been isolated from intestines poultry and Elevated aerotolerance may enhance survival ecosystem, thereby promoting transmission. However, invasive HAT isolates of have not reported bacteremia Case Summary A strain...
Abstract The rapid spread of influenza virus subtype H1N1 poses a great threat to million lives worldwide. To search for new anti-influenza compounds, we performed molecular docking and dynamics simulation identify potential traditional Chinese medicine (TCM) constituents that could block M2 channel activity. Quinic acid, genipin, syringic cucurbitine, fagarine, methyl isoferulate all have extremely well results as compared control amantadine. Further de novo drug design suggests derivatives...
Abstract We used metagenomic next-generation sequencing to longitudinally assess the gut microbiota and antimicrobial resistomes of international travelers clarify global exchange resistant organisms. Travel resulted in an increase resistance genes a greater proportion Escherichia species within microbial communities without impacting diversity.
A 59-year-old man with hypothyroidism and obesity was transferred from an outside hospital to our medical intensive care unit for management of acute hypoxemic respiratory failure due coronavirus disease 2019 (COVID-19). One day prior transfer, the patient required endotracheal intubation mechanical ventilation, heavy sedation neuromuscular blockade, norepinephrine hemodynamic support. His cardiac exam showed a regular rate rhythm 1+ peripheral pulses in all extremities capillary refill time...
A 94-year-old male presented to the emergency department with 3 days of fever and total body weakness. The patient’s past medical history was significant for coronary artery disease, type 2 diabetes mellitus, gastroesophageal reflux Parkinsonism, hypertension, chronic kidney disease. Physical examination revealed (38.7°C), mild elevation in blood pressure (138/62 mm Hg) a normal room air oxygen saturation 96%. Lung sounds were distant, no rales, wheezing, or rhonchi appreciated. patient...
Importance Blood culture (BC) use benchmarks in US hospitals have not been defined. Objective To characterize BC adult intensive care units (ICUs) and wards hospitals. Design, Setting, Participants A retrospective cross-sectional study of medical ICUs, medical-surgical wards, from acute the 4 geographic regions was conducted. Critical access hospitals, less than 6 months data, non-US were excluded. The included data September 1, 2019, to August 31, 2021. Data analyzed February 23 July 14,...
The study aimed to improve the job satisfaction among PNP person-nel by addressing issues in PNP's internal disciplinary mech-anisms. Dasmarinas Component City Police Station PCOs and PNCOs took part study, descriptive comparison research de-sign was used examine cleansing mechanism programs applied. data collected using a sur-vey questionnaire that consistent with Memorandum Cir-culars 2019-027 20-2020. Kusumawardani (2024) asserted work discipline increases satisfaction. If de-creases, so...
Abstract The swine influenza virus (H1N1) 2009 pandemic highlights the importance of having effective anti-viral strategies. Recently, oseltamivir (Tamiflu) resistant viruses are identified; which further emphasizes urgency in developing new antiviral agents. In replication cycle, viral surface glycoprotein, hemagglutinin, is responsible for entry into host cells. Hence, a potentially strategy to inhibit mechanism. To develop novel agent that inhibits entry, we analyzed 20,000 traditional...
To determine the role of pyridoxine in treatment diabetic peripheral neuropathy, 18 symptomatic patients were treated with vitamin B6 or placebo a double-blind controlled study. Only one patient had low plasma pyridoxal phosphate level at start After 4 mo hydrochloride (50 mg three times daily) 6 9 pyridoxine-treated and placebo-treated noted significant relief from their neuropathic symptoms. There was no difference between two groups regard to fasting glucose, motor nerve conduction...
Single-stranded oligonucleotides (SSOs) are a rapidly expanding class of therapeutics that comprises antisense oligonucleotides, microRNAs, and aptamers, with ten clinically approved molecules. Chemical modifications such as the phosphorothioate backbone 2′-O-methyl ribose can improve stability pharmacokinetic properties therapeutic SSOs, but they also lead to toxicity in vitro vivo through nonspecific interactions cellular proteins, gene expression changes, disturbed RNA processing, changes...
Maintenance in nature of Borrelia burgdorferi, the pathogenic bacterium that causes Lyme disease, requires transmission through an infectious cycle includes a tick vector and mammalian host. The genetic requirements for persistence these disparate environments have not been well defined. B. burgdorferi has complex genome composed chromosome >20 plasmids. Previous work demonstrated two plasmids, lp25 lp28–1, To investigate requirement same plasmids during infection, we experimentally infected...
Phenotypic identification of AmpC, KPC and extended-spectrum beta-lactamases (ESBLs) among members the Enterobacteriaceae remains challenging. This study compared Phoenix Automated Microbiology System (BD Diagnostics) with Clinical Laboratory Standards Institute confirmatory method to identify ESBL production 200 Escherichia coli Klebsiella pneumoniae clinical isolates. The system misclassified nearly half isolates as ESBL-positive, requiring manual testing for confirmation. Inclusion...