A5045368960- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Enterobacteriaceae and Cronobacter Research
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- Infections and bacterial resistance
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Nosocomial Infections in ICU
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Vector-borne infectious diseases
- Infection Control in Healthcare
- Streptococcal Infections and Treatments
- Plant Pathogenic Bacteria Studies
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
University of Virginia
2016-2025
University of Virginia Health System
2015-2024
Charlottesville Medical Research
2017-2024
University of Virginia Medical Center
2016-2023
Office of Infectious Diseases
2013-2021
Museum of Heilongjiang Province
2021
Engineering Associates (United States)
2017
Duke University Health System
2013
Maine Medical Center
2012
Maine Medical Center Research Institute
2006-2012
Abstract Background The Infectious Diseases Society of America is committed to providing up-to-date guidance on the treatment antimicrobial-resistant infections. This document focuses infections caused by extended-spectrum β-lactamase–producing Enterobacterales, AmpC β-lactamase-producing carbapenem-resistant Pseudomonas aeruginosa with difficult-to-treat resistance, Acinetobacter baumannii, and Stenotrophomonas maltophilia. updated replaces previous versions document. Methods A panel 6...
Treatment options for carbapenem-resistant Enterobacteriaceae (CRE) infections are limited and CRE remain associated with high clinical failure mortality rates, particularly in vulnerable patient populations. A Phase 3, multinational, open-label, randomized controlled trial (TANGO II) was conducted from 2014 to 2017 evaluate the efficacy/safety of meropenem-vaborbactam monotherapy versus best available therapy (BAT) CRE.A total 77 patients confirmed/suspected infection (bacteremia,...
Abstract Background Antimicrobial-resistant infections are commonly encountered in US hospitals and result significant morbidity mortality. This guidance document provides recommendations for the treatment of caused by extended-spectrum β-lactamase–producing Enterobacterales (ESBL-E), carbapenem-resistant (CRE), Pseudomonas aeruginosa with difficult-to-treat resistance (DTR-P. aeruginosa). Methods A panel 6 infectious diseases specialists expertise managing antimicrobial-resistant formulated...
Abstract Background Antimicrobial-resistant infections are commonly encountered in US hospitals and result significant morbidity mortality. This guidance document provides recommendations for the treatment of caused by extended-spectrum β-lactamase–producing Enterobacterales (ESBL-E), carbapenem-resistant (CRE), Pseudomonas aeruginosa with difficult-to-treat resistance (DTR-P. aeruginosa). Methods A panel 6 infectious diseases specialists expertise managing antimicrobial-resistant formulated...
The Infectious Diseases Society of America (IDSA) is committed to providing up-to-date guidance on the treatment antimicrobial-resistant infections. initial document infections caused by extended-spectrum β-lactamase producing Enterobacterales (ESBL-E), carbapenem-resistant (CRE), and Pseudomonas aeruginosa with difficult-to-treat resistance (DTR-P. aeruginosa) was published 17 September 2020. Over past year, there have been a number important publications furthering our understanding...
The recent widespread emergence of carbapenem resistance in Enterobacteriaceae is a major public health concern, as carbapenems are therapy last resort against this family common bacterial pathogens. Resistance genes can mobilize via various mechanisms, including conjugation and transposition; however, the importance mobility short-term evolution, such within nosocomial outbreaks, unknown. Using combination short- long-read whole-genome sequencing 281 blaKPC-positive isolates from single...
Immune dysregulation is characteristic of the more severe stages SARS-CoV-2 infection. Understanding mechanisms by which immune system contributes to COVID-19 severity may open new avenues treatment. Here, we report that elevated IL-13 was associated with need for mechanical ventilation in 2 independent patient cohorts. In addition, patients who acquired while prescribed Dupilumab, a mAb blocks and IL-4 signaling, had less disease. SARS-CoV-2–infected mice, neutralization reduced death...
Abstract The Infectious Diseases Society of America (IDSA) is committed to providing up-to-date guidance on the treatment antimicrobial-resistant (AMR) infections. This document focuses infections caused by extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E), AmpC (AmpC-E), carbapenem-resistant (CRE), Pseudomonas aeruginosa with difficult-to-treat resistance (DTR P. aeruginosa), Acinetobacter baumannii (CRAB), and Stenotrophomonas maltophilia. updated replaces previous versions...
ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as major causes of health care-associated infections worldwide. This diverse collection organisms with various resistance mechanisms is associated increased lengths hospitalization, costs care, morbidity, and mortality. The global spread CRE has largely been attributed to dissemination a dominant strain Klebsiella pneumoniae producing serine β-lactamase, termed K. carbapenemase (KPC). Here we report an outbreak KPC-producing...
ABSTRACT There have been an increasing number of reports implicating Gammaproteobacteria as often carrying genes drug resistance from colonized sink traps to vulnerable hospitalized patients. However, the mechanism transmission wastewater P-trap patients remains poorly understood. Herein we report use a designated hand-washing lab gallery model dispersion green fluorescent protein (GFP)-expressing Escherichia coli surrounding environment. We found no GFP-expressing E. directly basin or...
The global emergence of Klebsiella pneumoniae carbapenemase-producing K. (KPC-Kp) multilocus sequence type ST258 is widely recognized. Less known about the molecular and epidemiological details non-ST258 in setting an outbreak mediated by endemic plasmid. We describe interplay blaKPC plasmids strains their relationship to location acquisition a U.S. health care institution. Whole-genome sequencing (WGS) analysis was applied KPC-Kp clinical isolates collected from single institution over 5...
Carbapenem-resistant Enterobacteriaceae (CRE) represent a health threat, but effective control interventions remain unclear. Hospital wastewater sites are increasingly being highlighted as important potential reservoirs.
The dissemination of carbapenem resistance in Escherichia coli has major implications for the management common infections. bla KPC, encoding a transmissible carbapenemase (KPC), historically largely been associated with Klebsiella pneumoniae, predominant plasmid (pKpQIL), and specific transposable element (Tn4401, ~10 kb). Here we characterize genetic features KPC emergence global E. coli, 2008-2013, using both long- short-read whole-genome sequencing. Amongst 43/45 successfully sequenced...
This study aimed to assess the feasibility of using Oxford Nanopore Technologies (ONT) MinION long-read sequencer in reconstructing fully closed plasmid sequences from eight Enterobacteriaceae isolates six different species with populations varying complexity. Species represented were Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter cloacae, Serratia marcescens and oxytoca, ranging 1-11 plasmids sizes 2-330 kb. Isolates sequenced Illumina (short-read) ONT's...
The increasing prevalence of nosocomial carbapenemase-producing Enterobacteriaceae is a concern. However, the role environment in multispecies outbreaks remains poorly understood. There recognition that hospital wastewater plumbing may play role. Covers were installed on all hoppers (a "toilet-like" waste disposal system) adult intensive care units (ICUs) university hospital; additionally surgical ICU, sink trap heating and vibration devices also installed. Patient acquisitions Klebsiella...
We report a case of 24-year-old liver transplant recipient who developed hepatic artery thrombosis and graft failure, which was complicated by subphrenic abscess persistent
Wastewater-based monitoring for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at the individual building level could be an efficient, passive means of early detection new cases in congregate living settings, but this approach has not been validated. Preliminary samples were collected from a hospital and local municipal wastewater treatment plant. Molecular diagnostic methods compared side by to assess feasibility, performance, sensitivity. Refined sample collection processing...