A5045551151- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Fungal Infections and Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Urinary Tract Infections Management
- Infections and bacterial resistance
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Mycology
- Plant Pathogens and Fungal Diseases
- Vibrio bacteria research studies
- Nail Diseases and Treatments
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Plant Pathogenic Bacteria Studies
- Fungal Biology and Applications
- Streptococcal Infections and Treatments
- Nosocomial Infections in ICU
- Escherichia coli research studies
JMI Laboratories
2016-2025
University of Lisbon
2023
Merck & Co., Inc., Rahway, NJ, USA (United States)
2022
Park University
2022
Statens Serum Institut
2018
European Society of Clinical Microbiology and Infectious Diseases
2018
Tufts University
2010
Universidade Federal de São Paulo
2003-2008
University of Bristol
2004-2008
All Africa Leprosy Tuberculosis and Rehabilitation Training Centre
2006
Candida auris, a multidrug-resistant yeast that causes invasive infections, was first described in 2009 Japan and has since been reported from several countries. To understand the global emergence epidemiology of C. we obtained isolates 54 patients with auris infection Pakistan, India, South Africa, Venezuela during 2012–2015 type specimen Japan. Patient information available for 41 isolates. We conducted antifungal susceptibility testing whole-genome sequencing (WGS). Available clinical...
Background. Fluconazole (FLC) resistance is common in C. glabrata and echinocandins are often used as first-line therapy. Resistance to echinocandin therapy has been associated with FKS1 FKS2 gene alterations. Methods. We reviewed records of all patients bloodstream infection at Duke Hospital over the past decade (2001–2010) correlated treatment outcome minimum inhibitory concentration (MIC) results presence FKS mutations. For each isolate, MICs FLC (anidulafungin, caspofungin, micafungin)...
The emergence of antifungal resistance threatens effective treatment invasive fungal infection (IFI). Invasive candidiasis is the most common health care-associated IFI. We evaluated activity fluconazole (FLU) against 20 788 isolates Candida (37 species) collected from 135 medical centers in 39 countries (1997-2016). anidulafungin, caspofungin, and micafungin (MCF) was 15 308 worldwide (2006-2016).Species identification accomplished using phenotypic (1997-2001), genotypic, proteomic methods...
ABSTRACT The echinocandin class of antifungal agents is considered to be the first-line treatment bloodstream infections (BSI) due Candida glabrata . Recent reports BSI strains C. resistant both fluconazole and echinocandins are concern prompted us review experience two large surveillance programs, SENTRY Antimicrobial Surveillance Program for years 2006 through 2010 Centers Disease Control Prevention population-based conducted in 2008 2010. vitro susceptibilities 1,669 isolates fluconazole,...
ABSTRACT Among 39 carbapenem-resistant Enterobacteriaceae (2.7% overall; Escherichia coli , Enterobacter cloacae and Klebsiella pneumoniae strains) isolated in 2006 2007 India, 15 strains carried bla NDM-1 10 harbored a gene encoding variant of the carbapenemase OXA-48, named OXA-181 . One E. strain VIM-6 one K. carrying also possessed VIM-5 Multiple pulsed-field gel electrophoresis patterns clonal dissemination within among sites were observed. Isolates producing disseminated Indian health...
ABSTRACT The SENTRY Antimicrobial Surveillance Program monitors global susceptibility and resistance rates of newer established antifungal agents. We report the echinocandin triazole patterns for 3,418 contemporary clinical isolates yeasts molds. were obtained from 98 laboratories in 34 countries during 2010 2011. Yeasts not presumptively identified by CHROMagar, trehalose test, or growth at 42°C all molds sequence using internal transcribed spacer (ITS) 28S (yeasts) ITS, translation...
To evaluate the genetic relatedness and carbapenem resistance mechanisms among carbapenem-non-susceptible Pseudomonas aeruginosa collected during 2009-11 in 14 European Mediterranean countries.Doripenem-non-susceptible (MIC >2 mg/L) isolates were tested for susceptibility to imipenem, meropenem, doripenem, aztreonam, ceftazidime cefepime with without phenyl-arginine-β-naphthylamide (PAβN) (efflux inhibitor) and/or cloxacillin (AmpC inhibitor). Carbapenemase screening was performed by PCR...
Acinetobacter calcoaceticus-A. baumannii (Acb) complex and Stenotrophomonas maltophilia represent frequent causes of hospital-acquired infections. We evaluated the frequency resistance rates Acb S. isolates from medical centers enrolled in SENTRY Program.A total 13 752 6467 were forwarded to a monitoring laboratory by 259 participating sites Asia-Pacific region, Latin America, Europe, North America between 1997 2016. Confirmation species identification antimicrobial susceptibility testing...
Abstract Acinetobacter baumannii-calcoaceticus complex is the most commonly identified species in genus and it accounts for a large percentage of nosocomial infections, including bacteremia, pneumonia, infections skin urinary tract. A few key clones A. are currently responsible dissemination these organisms worldwide. Unfortunately, multidrug resistance common trait among due to their unrivalled adaptive nature. isolates can accumulate traits by plethora mechanisms, horizontal gene transfer,...
ABSTRACT Metallo-β-lactamase enzymes (MβL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. The objective of this study was develop a multiplex real-time PCR assay followed melt curve step for rapid detection and identification genes encoding MβL-type based on the amplicon melting peak. reference sequences all IMP VIM types, SPM-1, GIM-1, SIM-1 were downloaded from GenBank, primers designed obtain amplicons showing different sizes peak...
As part of the SENTRY Antimicrobial Surveillance Program in 2002, five multidrug-resistant Pseudomonas aeruginosa clinical isolates were detected with metallo-beta-lactamase (MbetaL) activity. The recovered from different patients a medical center located Dusseldorf, Germany. resistant determinant was isolated amplifying region between integrase and aacA4 gene cassette. Sequencing revealed novel MbetaL gene, designated bla(GIM-1). Additional analysis showed that GIM-1, comprising 250 amino...
ABSTRACT Community-onset (CO) candidemia, defined as a positive blood culture taken at or within 2 days of hospital admission, represents distinct clinical entity associated with substantial morbidity and mortality. Reference MIC results from the SENTRY Antimicrobial Surveillance Program (2008-2009) were analyzed to compare antifungal resistance patterns species distributions patients CO nosocomial bloodstream infections (BSI) in 79 medical centers. Among 1,354 episodes BSI, 494 (36.5%)...
Linezolid resistance has dominantly been mediated by mutations in 23S rRNA or ribosomal protein L4 genes. Recently, cfr demonstrated the ability to produce a phenotype of not only oxazolidinones, but also other antimicrobial classes (phenicols, lincosamides, pleuromutilins, and streptogramin A). We describe first detection cfr-mediated linezolid Staphylococcus aureus epidermidis recovered from human infection cases monitored during 2007 LEADER Program.
Antifungal testing results from the SENTRY Antimicrobial Surveillance Program (2008 to 2009) were analyzed for regional variations of invasive Candida species infections. Among 2,085 cases Asian-Pacific (APAC) (51 cases), Latin American (LAM) (348 European (EU) (750 and North (NAM) (936 cases) regions, albicans predominated (48.4%), followed by C. glabrata (18.0%), parapsilosis (17.2%), tropicalis (10.5%), krusei (1.9%). Resistance echinocandins (anidulafungin [2.4%] micafungin [1.9%])...
ABSTRACT Escherichia coli (328 isolates), Klebsiella pneumoniae (296), oxytoca (44), and Proteus mirabilis (33) isolates collected during 2012 from the nine U.S. census regions displaying extended-spectrum-β-lactamase (ESBL) phenotypes were evaluated for presence of β-lactamase genes, antimicrobial susceptibility profiles analyzed. The highest ESBL rates noted K. (16.0%, versus 4.8 to 11.9% other species) in Mid-Atlantic West South Central regions. CTX-M group 1 (including CTX-M-15) was...
The aim of this study was to evaluate the occurrence and dissemination acquired carbapenem-hydrolysing class D β-lactamase (class carbapenemase)- metallo-β-lactamase (MBL)-encoding genes among Acinetobacter spp. isolates recovered from medical centres in Asia-Pacific (APAC) region. During 2006–07, 41 located 10 countries APAC region forwarded a central monitoring site 544 isolates, which were tested for susceptibility by reference broth microdilution method. Isolates non-susceptible imipenem...
We evaluated the activity of meropenem-vaborbactam against contemporary nonfastidious Gram-negative clinical isolates, including Enterobacteriaceae isolates with resistance phenotypes and carbapenemase genotypes. Meropenem-vaborbactam (inhibitor at 8 μg/ml) comparators were susceptibility tested by reference broth microdilution methods 14,304 collected worldwide during 2014. Carbapenemase-encoding genes screened PCR sequencing. (MIC50/90, ≤0.015/0.06 inhibited 99.1 99.3% 10,426 ≤1 ≤2 μg/ml,...
Clinical breakpoints (CBPs) and epidemiological cutoff values (ECVs) have been established for several Candida spp. the newer triazoles echinocandins but are not yet available older antifungal agents, such as amphotericin B, flucytosine, or itraconazole. We determined species-specific ECVs B (AMB), flucytosine (FC) itraconazole (ITR) eight (30,221 strains) using isolates from 16 different laboratories in Brazil, Canada, Europe, United States, all tested by CLSI reference microdilution...