A5052948502- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Porphyrin Metabolism and Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Photosynthetic Processes and Mechanisms
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Cardiac electrophysiology and arrhythmias
- Neuroinflammation and Neurodegeneration Mechanisms
- Historical Studies of Medieval Iberia
- Organ Transplantation Techniques and Outcomes
- Neuroscience and Neural Engineering
- Heme Oxygenase-1 and Carbon Monoxide
- Photoreceptor and optogenetics research
- ATP Synthase and ATPases Research
- Architecture and Art History Studies
- Social Sciences and Policies
- Cellular transport and secretion
- Animal Nutrition and Physiology
- Ancient Mediterranean Archaeology and History
- Liver Disease and Transplantation
- Geology and Paleoclimatology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
University Health Network
2000-2025
University of Liverpool
2023
Cardiff University
2022
Nathan Kline Institute for Psychiatric Research
2019
Manhattan Psychiatric Center
2019
Sinclair Community College
2019
University of Manchester
1928-2015
University of Manitoba
2006
Canadian Bio-Systems (Canada)
2004
University of Toronto
1993-2002
Vaccines based on the spike protein of SARS-CoV-2 are a cornerstone public health response to COVID-19. The emergence hypermutated, increasingly transmissible variants concern (VOCs) threaten this strategy. Omicron (B.1.1.529), fifth VOC be described, harbours multiple amino acid mutations in spike, half which lie within receptor-binding domain. Here we demonstrate substantial evasion neutralization by BA.1 and BA.2 vitro using sera from individuals vaccinated with ChAdOx1, BNT162b2...
The membrane potential of cytoplasts, derived from human neutrophils, was depolarized by the activation superoxide-generating NADPH-dependent oxidase. extent depolarization inhibited diphenylene iodonium and therefore due directly to activity oxidase, which must be electrogenic. influenced alteration delta pH across cytoplast membrane, indicating that outward translocation H+ eventually compensates for superoxide generation. is enhanced Cd2+, a blocker currents, suggesting compensatory...
Summary: Purpose : In this study, we assessed the effects of topiramate (TPM) on high‐voltage‐activated calcium channel (HVACC) currents in vitro. Methods HVACC were recorded from rat dentate gyrus granule cells by using whole‐cell patch‐clamp techniques. The biophysical properties HVACCs used to separate voltage‐activated Ca 2+ into different subtypes. Three concentrations TPM tested: 1, 10, and 50 μ M . Results inhibited L‐type was more effective at 10 than , suggesting that there may be...
1. Anaerobic conditions are normally necessary for incorporation of iron into haems and only ferrous is used. After addition succinate to an incubation mixture containing intact or ultrasonically treated mitochondria, Fe(3+) used, but if no inhibitors prevent the transfer electrons from mitochondrial respiratory chain oxygen. 2. A dual-wavelength spectrophotometric assay ferrochelatase described that has been used continuous metal ions porphyrins. Constants given determination rates...
Peritoneal macrophages were elicited in rats by using casein as a stimulus; when stimulated with phorbol 12-myristate 13-acetate (PMA) they produced O2.-. Nearly 60% of the total cytochrome b had low Em,7.0 -247 mV, typical component found NADPH-dependent O2(.-)-generating oxidase neutrophils. The rate O2.- generation was 1.23 mol O2.-/s per b. Treatment intact diphenyleniodonium (DPI) at 0.9 microM caused 50% inhibition PMA-induced generation, little effect on mitochondrial respiratory...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnnular and nonannular binding sites for cholesterol associated with the nicotinic acetylcholine receptorOwen T. Jones Mark G. McNameeCite this: Biochemistry 1988, 27, 7, 2364–2374Publication Date (Print):April 5, 1988Publication History Published online1 May 2002Published inissue 5 April 1988https://pubs.acs.org/doi/10.1021/bi00407a018https://doi.org/10.1021/bi00407a018research-articleACS PublicationsRequest reuse permissionsArticle...
The internal pH (pHi) of cytoplasts, derived from human neutrophils, falls 0.05 units upon activation the superoxide-generating NADPH oxidase. decrease in pHi is absent diphenyleneiodonium-treated cytoplasts and therefore it likely to arise directly activity addition amiloride, diminish Na+/H+ exchanger, enhanced extent acidification but not initial rate. However electroneutral exchanger cannot be a contributor H+ efflux compensate for charge translocated by In presence Cd ions or...
Previous work has shown that human mesangial cells (HMC) are capable of low rates generation reactive oxygen species for considerable periods time. In this communication, the presence components an NADPH oxidase-like system, more commonly associated with phagocytic leukocytes, is shown. The ability HMC to generate levels superoxide may have important implications in cellular signaling general and contribute glomerular injury. Spectroscopic analysis membranes revealed a low-potential...
Voltage-dependent Ca 2+ channels (VDCCs) are modulators of synaptic plasticity, oscillatory behavior, and rhythmic firing in brain regions such as the hippocampus. The distribution lateral mobility VDCCs on CA1 hippocampal neurons have been determined with biologically active fluorescent biotinylated derivatives selective probe ω-conotoxin conjunction circular dityndallism, digital fluorescence imaging, photobleach recovery microscopy. On noninnervated cell bodies, were found to be organized...
1. In rat heart, three K+ channel genes that encode inactivating transient outward (ITO)-like currents are expressed. During development the predominant mRNA species switches from Kv1.4 to Kv4.2 and Kv4.3. However, no functional correlate of this isoform switch has been reported. We investigated action potential characteristics ITO in cultured neonatal ventricular myocytes adult hearts. further examined whether changes gene expression associated electrophysiology occurs during could be...
Recently, we have shown that human glomerular mesangial cells (HMCs) release oxygen radicals from the plasma membrane in response to cytokines. Now used diphenylene iodonium, a covalent binding inhibitor of activated 45-kDa flavoprotein, neutrophils radiolabeled with 125I and could identify protein band separated HMC fraction. Low temperature difference spectroscopy showed peak absorbance at 428 558 nm. Direct potentiometry membranes (-340 -160 mV) presence low potential cytochrome (76...
EBV-transformed B lymphocyte cell lines can generate superoxide, using an electron transport chain homologous, or even identical, to phagocytic NADPH-oxidase. We searched for normal, not virally transformed, lymphocytes with analogous properties, tonsils as the source of cells. Unseparated tonsillar leukocytes contained cells capable PMA-triggered superoxide dismutase-inhibitable reduction nitroblue tetrazolium (NBT+ cells) well in excess phagocytes (18.9 +/- 6.4% NBT+ 1.3 0.9% granulocytes...
The effects of diet type (corn- vs. wheat-based) and multicarbohydrase addition on growth performance, digesta pH viscosity, intestinal populations Clostridium perfringens lactic acid bacteria, gut lesion score (from 0 to 4, where = no gross lesions, 4 severe extensive necrosis) broiler chickens during oral challenge with C. (none or 108 cfu/bird d 13) were studied in a 39-d experiment. A total 1,216 male Ross-308 was assigned 8 dietary treatments randomized complete block design providing...
The stimulation of O2.- generation by phorbol 12-myristate 13-acetate (PMA) in human neutrophil-derived cytoplasts was inhibited a variety phospholipase A2 inhibitors concentration-dependent manner. Inhibition found to be independent the order addition inhibitor and PMA. most potent inhibitor, RO 31-4639, with an IC50 value (concentration causing 50% inhibition) 1.5 microM. either arachidonic acid or SDS, presence inhibitors, able restore generation. results suggest that acid, released A2,...
An in vitro incubation study was carried out to determine whether various carbohydrase preparations contained appropriate activities target nonstarch polysaccharides (NSP) of full-fat flaxseed. Enzyme C (cellulase, 340 U/g), XG (xylanase, 63,600 U/g and glucanase, 48,300 P (pectinase, 10,000 MC (mannanase, 10,900 cellulase, 600 U/ g), alone combination (C + P, XG, MC, MC), were evaluated. Triplicate samples defatted flaxseed meal (0.1 g) incubated with 1% single enzymes or combinations at 45...
An experiment was conducted to investigate the effects of a multicarbohydrase enzyme on egg production parameters, nutrient digestibility, and fatty acid composition in Hy-Line CV-20 laying hens (39 63 wk age) fed diets containing 150 g/kg diet canola seed, flaxseed, or Linpro (flaxseed:peas, 1:1 wt/wt). The effect each parameter also evaluated. Hens consuming seed had greater production, lower feed consumption, therefore better conversion than those flaxseed diets. Enzyme supplementation...
The contribution of voltage-dependent ion channels to nerve function depends upon their cell-surface distributions. Nevertheless, the mechanisms underlying channel localization are poorly understood. Two phenomena appear particularly important: clustering by membrane-associated guanylate kinases (MAGUKs), such as PSD-95, and regional stabilization proteins differential suppression endocytosis. Could these be related? To test this possibility we examined effect PSD-95 on internalization rate...
The response of nerve cells to synaptic inputs and the propagation this activation is critically dependent on cell-surface distribution ion channels. In hippocampus, Ca2+ influx through N-methyl-D-aspartate receptors (NMDAR) and/or voltage-dependent calcium channels dendrites thought be involved in long-term potentiation, neurite outgrowth, epileptogenesis, synaptogenesis, cell death. We report that conantokin-G (CntxG), a peptide from Conus geographus venom, competitively blocked with high...
The (Ca2+-Mg2+)-ATPase from sarcoplasmic reticulum has been reconstituted into phospholipid bilayers of different fatty acyl chain length and degree unsaturation, ATPase activity was found to vary about 10-fold. Order parameters for these lipids measured using the ESR probe 5-doxylstearic acid varied 0.5 0.6 at 37 degrees C. There no correlation between order parameter activity, we conclude that structure is a more important determinant protein than membrane fluidity.
Abstract Dysfunction of surviving axons which traverse the site spinal cord injury (SCI) has been linked to altered sensitivity K + channel blocker 4‐aminopyridine (4‐AP) and appears contribute post‐traumatic neurological deficits although underlying mechanisms remain unclear. In this study, sucrose gap electrophysiology in isolated dorsal column strips, Western blotting confocal immunofluorescence microscopy were used identify channels associated with axonal dysfunction after chronic (6–8...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA minimum number of lipids are required to support the functional properties nicotinic acetylcholine receptorOwen T. Jones, James H. Eubanks, Julie P. Earnest, and Mark G. McNameeCite this: Biochemistry 1988, 27, 10, 3733–3742Publication Date (Print):May 1, 1988Publication History Published online1 May 2002Published inissue 1 1988https://pubs.acs.org/doi/10.1021/bi00410a032https://doi.org/10.1021/bi00410a032research-articleACS PublicationsRequest...