Sarah E. McDonald
A5088591556- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Reproductive System and Pregnancy
- Long-Term Effects of COVID-19
- COVID-19 and healthcare impacts
- Ectopic Pregnancy Diagnosis and Management
- Animal Virus Infections Studies
- Endometriosis Research and Treatment
- Hepatitis C virus research
- Estrogen and related hormone effects
- COVID-19 epidemiological studies
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Respiratory Support and Mechanisms
- COVID-19 and Mental Health
- Hepatitis Viruses Studies and Epidemiology
- Sepsis Diagnosis and Treatment
- Pregnancy and preeclampsia studies
- Reproductive tract infections research
- Hepatitis B Virus Studies
- Recreation, Leisure, Wilderness Management
- Nosocomial Infections in ICU
- Respiratory viral infections research
Johns Hopkins Hospital
2024
MRC University of Glasgow Centre for Virus Research
2017-2024
Medical Research Council
2022-2023
Great Ormond Street Hospital for Children NHS Foundation Trust
2023
International Severe Acute Respiratory and Emerging Infection Consortium
2023
St Mary's Hospital
2023
University of Edinburgh
2006-2022
University of Sheffield
2022
Roslin Institute
2022
University of Liverpool
2021
An outbreak of acute hepatitis unknown aetiology in children was reported Scotland1 April 2022 and has now been identified 35 countries2. Several recent studies have suggested an association with human adenovirus this outbreak, a virus not commonly associated hepatitis. Here we report detailed case–control investigation find between adeno-associated 2 (AAV2) infection host genetics disease susceptibility. Using next-generation sequencing, PCR reverse transcription, serology situ...
Summary Temperature-sensitive mutants of adenovirus type 5 have been isolated from virus stocks mutagenized by nitrous acid, hydroxylamine and 5-bromodeoxyuridine. The frequency such in the surviving fractions acid hydroxylamine-treated was 8.4 9.6%, respectively, while it around 0.55% grown Most tested so far appear to be relatively stable show little evidence excessive leakiness or back mutation, will suitable for genetic analysis. Preliminary experiments good complementation between four...
Summary paragraph An outbreak of acute hepatitis unknown aetiology in children was first reported Scotland April 2022. 1 Cases aged <16 years have since been identified 35 countries. 2 Here we report a detailed investigation 9 early cases and 58 control subjects. Using next-generation sequencing real-time PCR, adeno-associated virus (AAV2), detected the plasma 9/9 liver 4/4 patients but 0/13 sera/plasma age-matched healthy controls, 0/12 with adenovirus (HAdV) infection normal function...
Background Embryo retention in the Fallopian tube (FT) is thought to lead ectopic pregnancy (EP), a considerable cause of morbidity. In mice, genetic/pharmacological silencing cannabinoid receptor Cnr1, encoding CB1, causes embryos oviduct. The role endocannabinoids tubal implantation humans not known. Methods and Findings Timed FT biopsies (n = 18) were collected from women undergoing gynecological procedures for benign conditions. Endometrial whole blood surgery EP 11); management...
Sex steroid hormone receptor (SHR) dynamics are well-documented in human endometrium but have not been comprehensively studied Fallopian tube (FT).The aim of the study was to compare expression patterns and hormonal regulation SHR FT with that described determine whether is altered women ectopic pregnancy (EP).Tissue analyzed cultured.Women undergoing surgery for benign gynecological conditions (n = 14) EP 6) participated study.Quantitative RT-PCR immunohistochemistry were used mRNA protein...
Tubal ectopic pregnancy is common, but accurate diagnosis difficult and costly. There currently no serum test to differentiate tubal from intrauterine implantation, an effective biomarker of would be a major clinical advance.A key feature successful implantation the establishment supportive vascular network, this has been associated with activity placental growth factor (PIGF). We hypothesized that local decidual environment facilitates PIGF-dependent angiogenesis pathway not active in...
Abstract Background Predicting bed occupancy for hospitalised patients with COVID-19 requires understanding of length stay (LoS) in particular types. LoS can vary depending on the patient’s “bed pathway” - sequence transfers individual between types during a hospital stay. In this study, we characterise these pathways, and their impact predicted occupancy. Methods We obtained data from University College Hospital (UCH) ISARIC4C Clinical Information Network (CO-CIN) who required care general...
Expression of secretory leukocyte protease inhibitor
The endometrium in early pregnancy undergoes decidualization and functional changes induced by local trophoblast, which are not fully understood. We hypothesized that from tubal ectopic (EP) could be interrogated to identify novel genes pathways involved these processes. Gestation-matched was collected women with EP (n = 11) intrauterine pregnancies (IUP) 13). RNA extracted the tissue. In addition, tissues were prepared for histological analysis degree of decidualization. compared a) samples...
Abstract SARS-CoV-2, the causative agent of COVID-19, emerged in Wuhan, China December 2019 and spread rapidly throughout world. Understanding introductions this new coronavirus different settings may assist control efforts establishment frameworks to support rapid response future infectious disease outbreaks. We investigated first four weeks emergence SARS-CoV-2 virus Scotland after case reported on 1st March 2020. obtained full genome sequences from 452 individuals with a...
Abstract The second SARS virus, SARS-CoV-2, emerged in December 2019, and within a month was globally distributed. It first introduced into Scotland February 2020 associated with returning travellers visitors. By March it circulating communities across the UK, to control COVID-19 cases, prevent overwhelming of National Health Service (NHS), ‘lockdown’ on 23rd restriction people’s movements. To augment public health efforts large-scale genome epidemiology effort (as part Genomics UK (COG-UK)...
COVID-19 is clinically characterised by fever, cough, and dyspnoea. Symptoms affecting other organ systems have been reported. However, it the clinical associations of different patterns symptoms which influence diagnostic therapeutic decision-making. In this study, we applied clustering techniques to a large prospective cohort hospitalised patients with identify meaningful sub-phenotypes. We obtained structured data on 59,011 in UK (the ISARIC Coronavirus Clinical Characterisation...
The SARS-CoV-2 pandemic enables the analysis of immune responses induced against a novel coronavirus infecting immunologically naïve individuals. This provides an opportunity for and associations with age, sex disease severity. Here we measured array solid-phase binding antibody viral neutralising Ab (nAb) in participants (n=337) ISARIC4C cohort characterised their correlation peak severity during acute infection early convalescence. Overall, Double Antigen Binding Assay (DABA) to receptor...
Abstract The ongoing SARS-CoV-2 pandemic was initially managed by non-pharmaceutical interventions such as diagnostic testing, isolation of positive cases, physical distancing and lockdowns. advent vaccines has provided crucial protection against SARS-CoV-2. Neutralising antibody (nAb) responses are a key correlate protection, therefore measuring nAb is essential for monitoring vaccine efficacy. Fingerstick dried blood spots (DBS) ideal use in large-scale sero-surveillance because they...
We investigated whether the repulsive SLIT/ROBO pathway is expressed in endometrium and negatively regulated during implantation. also examined deficient expression Fallopian tube (FT) may predispose to ectopic pregnancy (EP). Endometrium (n = 21) FT 17) were collected across menstrual cycle from fertile women with regular cycles. Decidualized 6) was obtained undergoing termination, EP. quantified by reverse transcription–PCR protein localized immunohistochemistry. The regulation of vitro,...
Hepatitis C virus (HCV) NS3 is a multifunctional protein with both protease and helicase activities has been shown to interact host cell proteins. It that present in the hepatocytes from patients chronic HCV infection by using anti-NS3 antisera. detectable approximately 4% of these patients. In most infected cells, cytoplasm; however, minority HCV-infected cytoplasm nucleus or on its own are positive for NS3. The presence nuclei chronically indicates may play role other than replication,...
Patients requiring haemodialysis are at increased risk of serious illness with SARS-CoV-2 infection. To improve the understanding transmission risks in six Scottish renal dialysis units, we utilised rapid whole-genome sequencing data generated by COG-UK consortium.
There are concerns of reduced pregnancy rates with the use gonadotrophin-releasing hormone antagonists (GnRH antagonists) in IVF/ICSI cycles. Sex steroids and their metabolizing enzymes endometrium may play a vital role embryo implantation. This study has evaluated levels localization sex-steroid receptors enzymes, 3beta-hydroxysteroid dehydrogenases (3betaHSD) selected 17beta-HSD (17betaHSD), mid-luteal women treated GnRH antagonist (Cetrorelix) recombinant FSH (rFSH; Gonal-F) luteal phase...