A5019965773- Reproductive System and Pregnancy
- Clinical Laboratory Practices and Quality Control
- Ectopic Pregnancy Diagnosis and Management
- Diabetes Management and Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- SARS-CoV-2 detection and testing
- Endometriosis Research and Treatment
- Gestational Trophoblastic Disease Studies
- Quality and Safety in Healthcare
- Blood Coagulation and Thrombosis Mechanisms
- Meta-analysis and systematic reviews
- Pharmaceutical studies and practices
- Hemodynamic Monitoring and Therapy
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Blood transfusion and management
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- SARS-CoV-2 and COVID-19 Research
- Non-Invasive Vital Sign Monitoring
- Reproductive Health and Contraception
- Body Contouring and Surgery
- Potassium and Related Disorders
- Blood donation and transfusion practices
- Peptidase Inhibition and Analysis
Canadian Electricity Association
2015-2025
University of Ottawa
2015-2025
Ottawa Hospital
2013-2024
University of Saskatchewan
2017-2018
University Health Network
2012-2017
University of Toronto
1979-2017
Carleton University
2015
SickKids Foundation
2013
Hospital for Sick Children
2012-2013
Mount Sinai Hospital
2006-2012
Kallikreins (KLKs) are a group of 15 secreted serine proteases. Some KLKs established or candidate cancer biomarkers, but for most the physiological function is unknown. We characterized protein and mRNA abundance patterns all in multiple panels human tissues biological fluids.We used sensitive specific sandwich-type ELISAs each KLK. Reverse transcription PCR was transcript amplification. Multiple tissue extracts (adult fetal) were tested, along with various fluids.Quantitative expression...
Human cervico-vaginal fluid (CVF) is a mixture of fluids originating from the vagina, cervix, endometrium, and oviduct. CVF has been shown to play an important role in protecting vagina infection. We used “bottom-up” proteomic approaches characterize protein repertoire human CVF. applied two different sample prefractionation methods, one-dimensional-SDS-PAGE (1D-SDS-PAGE) strong cation-exchange chromatography, followed by LC−MS/MS bioinformatic analysis. identified total 685 proteins. Strong...
Primary aldosteronism, characterized by overt renin-independent aldosterone production, is a common but underrecognized form of hypertension and cardiovascular disease. Growing evidence suggests that milder subclinical forms primary aldosteronism are highly prevalent, yet their contribution to disease not well characterized.
Abstract The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Committee on Point-of-Care Testing (C-POCT) supports the use point-of-care testing (POCT) outside hospital setting performed by healthcare professionals without formal laboratory education because its numerous benefits. However, these benefits are associated with risks that must be managed, to ensure provision reliable test results minimize harm patient. Healthcare professionals, local regulatory...
Human tissue kallikrein 14 (KLK14) is a novel extracellular serine protease. Clinical data link KLK14 expression to several diseases, primarily cancer; however, little known of its (patho)-physiological role. To functionally characterize KLK14, we expressed and purified recombinant in mature proenzyme forms determined pattern, specificity, regulation, vitro substrates. By using our immunoassay, the normal and/or diseased skin, breast, prostate, ovary contained highest concentration KLK14....
Chlamydia trachomatis and smoking are major risk factors for tubal ectopic pregnancy (EP), but the underlying mechanisms of these associations not completely understood. Fallopian tube (FT) from women with EP exhibit altered expression prokineticin receptors 1 2 (PROKR1 PROKR2); increases FT PROKR1, resulting in a microenvironment predisposed to EP. We hypothesize that C. also predisposes by altering PROKR have investigated this examining NFκB activation via ligation Toll-like receptor (TLR)...
Abstract Background Point-of-care tests (POCT) for haemoglobin are increasingly used to guide intraoperative transfusion. However, their accuracy compared central laboratory is unknown. The objective was perform a systematic review and meta-analysis of method comparison studies assessing the POCT versus in patients undergoing surgery. Methods Electronic databases were searched from inception April 2020 (updated August 2023). Any methodological approach comparing measurements between surgery...
Point-of-care testing devices to measure haemoglobin (Hgb) frequently inform transfusion decision-making in surgery. This study aimed examine their accuracy surgery, focusing on Hgb concentrations of 60-100 g L was a prospective diagnostic cohort focused method comparison, conducted at two academic hospitals. Consecutive patients undergoing noncardiac surgery and requiring point-of-care measurements were eligible. from arterial central venous blood samples measured concurrently using three...
The extent of intersubject variation in diazepam free fraction was measured fasting plasma 74 unrelated subjects. Free differences between subjects were significant and ranged from 0.97% to 1.99%. Diazepam 29 males normally distributed about a mean 1.25% (range, 1.05% 1.47%), but the distribution females skewed higher fractions 40% had values above highest males. Albumin concentration (r = −0.27, p < 0.002) age 0.44, 0.001) only accounted for small part variation. Within‐pair variances...
Tubal ectopic pregnancy is common, but accurate diagnosis difficult and costly. There currently no serum test to differentiate tubal from intrauterine implantation, an effective biomarker of would be a major clinical advance.A key feature successful implantation the establishment supportive vascular network, this has been associated with activity placental growth factor (PIGF). We hypothesized that local decidual environment facilitates PIGF-dependent angiogenesis pathway not active in...
Abstract Human tissue kallikrein-related peptidases (KLKs) are 15 hormonally regulated genes on chromosome 19q13.4 encoding secreted serine proteases. Many KLKs expressed throughout the female reproductive system and found in cervico-vaginal fluid (CVF). Immunohistochemistry was performed to determine KLK localization (fallopian tube, endometrium, cervix vagina tissues). levels were measured CVF saliva over menstrual cycle study whether by hormonal changes during cycle. In vitro cleavage...
The endometrium in early pregnancy undergoes decidualization and functional changes induced by local trophoblast, which are not fully understood. We hypothesized that from tubal ectopic (EP) could be interrogated to identify novel genes pathways involved these processes. Gestation-matched was collected women with EP (n = 11) intrauterine pregnancies (IUP) 13). RNA extracted the tissue. In addition, tissues were prepared for histological analysis degree of decidualization. compared a) samples...
Down syndrome (DS) is one of the most common chromosomal abnormalities affecting about 1 every 700 fetuses. Current screening strategies have detection rates 90-95% at a 5% false positive rate. The aim this study was to discover new biomarkers DS in amniotic fluid by using multiplex selected reaction monitoring assay. Nine proteins were analyzed: CEL, CPA1, MUC13, CLCA1, MUC5AC, PLUNC, and HAPLN1, CGB as control serotransferrin negative control. One proteotypic peptide for each protein...
Lymphoid and myeloid cell populations in human endometrium are well-documented known to play important roles providing immune tolerance, controlling trophoblast invasion, mediating vascular remodeling. Immune the Fallopian tube have not been comprehensively studied. The aim of this study was characterize lymphoid non-pregnant determine whether they altered from women with ectopic pregnancy. analyzed by flow cytometry immunohistochemistry. Populations CD3+ (CD4+ CD8+) lymphocytes, LIN1-HLADR+...