A5068013870- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Analytical Chemistry and Chromatography
- Gut microbiota and health
- Mass Spectrometry Techniques and Applications
- Advanced Chemical Sensor Technologies
- SARS-CoV-2 and COVID-19 Research
- Advanced Proteomics Techniques and Applications
- Traditional Chinese Medicine Studies
- Metabolism and Genetic Disorders
- Genetic diversity and population structure
- COVID-19 Clinical Research Studies
- Fish Ecology and Management Studies
- Long-Term Effects of COVID-19
- Drug Transport and Resistance Mechanisms
- Tryptophan and brain disorders
- Bariatric Surgery and Outcomes
- Nutritional Studies and Diet
- Bacteriophages and microbial interactions
- Neuropeptides and Animal Physiology
- Formal Methods in Verification
- Regulation of Appetite and Obesity
- Alzheimer's disease research and treatments
- Neurological Disease Mechanisms and Treatments
Imperial College London
2015-2024
Bruker (United Kingdom)
2023-2024
Auburn University
2010-2023
International Severe Acute Respiratory and Emerging Infection Consortium
2023
Hammersmith Hospital
2014-2023
National Computing Centre (United Kingdom)
2023
University of Copenhagen
2023
Imperial College Healthcare NHS Trust
2023
National Health Service
2023
St Mary's Hospital
2023
The 2,160,837–base pair genome sequence of an isolate Streptococcus pneumoniae , a Gram-positive pathogen that causes pneumonia, bacteremia, meningitis, and otitis media, contains 2236 predicted coding regions; these, 1440 (64%) were assigned biological role. Approximately 5% the is composed insertion sequences may contribute to rearrangements through uptake foreign DNA. Extracellular enzyme systems for metabolism polysaccharides hexosamines provide substantial source carbon nitrogen S. also...
Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It a minimal metabolism and little genomic redundancy. Consequently, its is expected to close approximation set genes needed sustain bacterial life. Using global transposon mutagenesis, we isolated characterized gene disruption mutants for 100 different nonessential protein-coding genes. None 43 RNA-coding were disrupted. Herein, identify 382 482 M. as essential, plus five sets disrupted encode...
There is mounting evidence for a connection between the gut and Parkinson's disease (PD). Dysbiosis of microbiota could explain several features PD.
Rapid evaporative ionization mass spectrometry (REIMS) is an emerging technique that allows near-real-time characterization of human tissue in vivo by analysis the aerosol ("smoke") released during electrosurgical dissection. The coupling REIMS technology with electrosurgery for diagnostics known as intelligent knife (iKnife). This study aimed to validate applying it fresh samples ex and demonstrate translation real-time use a surgical environment. A variety from 302 patients were analyzed...
The completion of the 5,373,180-bp genome sequence marine psychrophilic bacterium Colwellia psychrerythraea 34H, a model for study life in permanently cold environments, reveals capabilities important to carbon and nutrient cycling, bioremediation, production secondary metabolites, cold-adapted enzymes. From genomic perspective, adaptation is suggested several broad categories involving changes cell membrane fluidity, uptake synthesis compounds conferring cryotolerance, strategies overcome...
The 2,160,267 bp genome sequence of Streptococcus agalactiae , the leading cause bacterial sepsis, pneumonia, and meningitis in neonates U.S. Europe, is predicted to encode 2,175 genes. Genome comparisons among S. pneumoniae pyogenes other completely sequenced genomes identified genes specific streptococci . These silico analyses, combined with comparative hybridization experiments between serotype V strain 2603 V/R 19 strains from several serotypes using whole-genome microarrays, revealed...
Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other in Rickettsiales order. spp. display a unique large expansion immunodominant outer membrane proteins facilitating antigenic variation. All have diminished ability synthesize amino acids compared their...
Proton nuclear magnetic resonance (NMR)-based metabolic phenotyping of urine and blood plasma/serum samples provides important prognostic diagnostic information permits monitoring disease progression in an objective manner. Much effort has been made recent years to develop NMR instrumentation technology allow the acquisition data effective, reproducible, high-throughput approach that allows study general population from epidemiological collections for biomarkers risk. The challenge remains...
Viruses are abundant ubiquitous members of microbial communities and in the marine environment affect population structure nutrient cycling by infecting lysing primary producers. Antarctic lakes microbially dominated ecosystems supporting truncated food webs which viruses exert a major influence on loop. Here we report discovery virophage (relative recently described Sputnik virophage) that preys phycodnaviruses infect prasinophytes (phototrophic algae). By performing metaproteogenomic...
Exploratory or untargeted ultra performance liquid chromatography-mass spectrometry (UPLC-MS) profiling offers an overview of the complex lipid species diversity present in blood plasma. Here, we evaluate and compare eight sample preparation protocols for optimized plasma extraction measurement by UPLC-MS profiling, including four protein precipitation methods (i.e., methanol, acetonitrile, isopropanol, isopropanol-acetonitrile) liquid-liquid extractions methanol combined with chloroform,...
We report an extensive 600 MHz NMR trial of quantitative lipoprotein and small-molecule measurements in human blood serum plasma. Five centers with eleven spectrometers were used to analyze 98 samples including 20 quality controls (QCs), 37 commercially sourced, paired plasma samples, two National Institute Science Technology (NIST) reference material 1951c replicates. Samples analyzed using rigorous protocols for sample preparation experimental acquisition. A commercial subclass analysis...
Bile acids are important end products of cholesterol metabolism. While they have been identified as key factors in lipid emulsification and absorption due to their detergent properties, bile also shown act signaling molecules intermediates between the host gut microbiota. To further investigation acid functions humans, an advanced platform for high throughput analysis is essential. Herein, we describe development application a 15 min UPLC procedure separation species from human biofluid...
Abstract Markers of biological aging have potential utility in primary care and public health. We developed a model age based on untargeted metabolic profiling across multiple platforms, including nuclear magnetic resonance spectroscopy liquid chromatography–mass spectrometry urine serum, within large sample ( N = 2,239) from the UK Airwave cohort. validated subset predictors Finnish cohort repeat measurements 2,144 individuals. investigated determinants accelerated aging, lifestyle...
BackgroundCOVID-19 is a multisystem disease and patients who survive might have in-hospital complications. These complications are likely to important short-term long-term consequences for patients, health-care utilisation, system preparedness, society amidst the ongoing COVID-19 pandemic. Our aim was characterise extent effect of complications, particularly in those survive, using International Severe Acute Respiratory Emerging Infections Consortium WHO Clinical Characterisation Protocol...
Alzheimer's disease (AD) is characterized by three primary pathologies in the brain: amyloid plaques, neurofibrillary tangles, and neuron loss. Mouse models have been useful for studying components of AD but are limited their ability to fully recapitulate all pathologies. We crossed APPSwDI transgenic mouse, which develops β (Aβ)-protein deposits only, with a nitric oxide synthase 2 (NOS2) knock-out no AD-like pathology. APPSwDI/NOS2 −/− mice displayed impaired spatial memory compared mice,...
ABSTRACT The complex human T-cell leukemia virus type 1 (HTLV-1) retrovirus encodes several proteins that are unique to the within its 3′-end region. Among them, viral transactivator Tax and posttranscriptional regulator Rex well characterized, both positively regulate HTLV-1 expression. Less is known about other regulatory encoded in this region of provirus, including recently discovered HBZ protein. has been shown negatively basal Tax-dependent transcription through ability interact with...
To better understand the molecular mechanisms underpinning physiological variation in human populations, metabolic phenotyping approaches are increasingly being applied to studies involving hundreds and thousands of biofluid samples. Hyphenated ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) has become a fundamental tool for this purpose. However, seemingly inevitable need analyze large multiple analytical batches UPLC-MS analysis poses challenge data quality which been...
Abstract Background Chemical and biological processes dictate an individual organism's ability to recognize respond other organisms. A small but growing body of evidence suggests that plants may be capable recognizing responding neighboring in a species specific fashion. Here we tested whether or not individuals the invasive exotic weed, Centaurea maculosa , would modulate their defensive strategy response different plant neighbors. Results In greenhouse, C. were paired with either...
Estimation of statistical power and sample size is a key aspect experimental design. However, in metabolic phenotyping, there currently no accepted approach for these tasks, large part due to the unknown nature expected effect. In such hypothesis free science, neither number or class important analytes nor effect are known priori. We introduce new approach, based on multivariate simulation, which deals effectively with highly correlated structure high-dimensionality phenotyping data. First,...
Matrix‐assisted laser desorption ionisation imaging mass spectrometry (MALDI‐MSI) is a rapidly advancing technique for intact tissue analysis that allows simultaneous localisation and quantification of biomolecules in different histological regions interest. This approach can potentially offer novel insights into tumour microenvironmental (TME) biochemistry. In this study we employed MALDI‐MSI to evaluate fresh frozen sections colorectal cancer (CRC) adjacent healthy mucosa obtained from 12...
Abstract Background Both serotonergic signalling disruption and systemic inflammation have been associated with the pathogenesis of Alzheimer’s disease (AD). The common denominator linking two is catabolism essential amino acid, tryptophan. Metabolism via tryptophan hydroxylase results in serotonin synthesis, whilst metabolism indoleamine 2,3-dioxygenase (IDO) kynurenine its downstream derivatives. IDO reported to be activated times host therefore thought influence both pathways. To...