A5073259394- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Galectins and Cancer Biology
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Chromosomal and Genetic Variations
- NF-κB Signaling Pathways
- CRISPR and Genetic Engineering
- Signaling Pathways in Disease
- RNA Research and Splicing
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Prenatal Screening and Diagnostics
- Renal and related cancers
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
Université du Québec à Montréal
2014-2024
Université de Montréal
2022
Toxicologie, Pharmacologie et Signalisation Cellulaire
2010
Université Laval
1997-2006
Centre hospitalier universitaire de Québec
1997-2006
Centre National de la Recherche Scientifique
2006
Wilfrid Laurier University
2004
Centre hospitalier de l'Université Laval
1996-2003
Armand Frappier Museum
1991
Institut National de la Recherche Scientifique
1991
Exosomes are extracellular vesicles that mediate intercellular communication and involved in several biological processes. The objective of our study was to determine whether endogenous retrovirus group WE, member l (ERVWE1)/syncytin-1 FRD, 1 (ERVFRDE1)/syncytin-2, encoded by human (HERV) envelope (env) genes, present at the surface exosomes produced placenta-derived villous cytotrophoblasts they play a role cellular uptake exosomes. In addition, we sought these proteins various abundances...
ABSTRACT The complex human T-cell leukemia virus type 1 (HTLV-1) retrovirus encodes several proteins that are unique to the within its 3′-end region. Among them, viral transactivator Tax and posttranscriptional regulator Rex well characterized, both positively regulate HTLV-1 expression. Less is known about other regulatory encoded in this region of provirus, including recently discovered HBZ protein. has been shown negatively basal Tax-dependent transcription through ability interact with...
Abstract Background Antisense transcription in retroviruses has been suggested for both HIV-1 and HTLV-I, although the existence coding potential of these transcripts remain controversial. Thorough characterization is required to demonstrate gain insight into their role retrovirus biology. Results This report provides first complete an antisense retroviral transcript that encodes previously described HTLV-I HBZ protein. In this study, we show HBZ-encoding initiate 3' long terminal repeat...
Abstract Background We and others have recently demonstrated that the human retrovirus HTLV-I was producing a spliced antisense transcript, which led to synthesis of HBZ protein. The objective present study demonstrate existence transcription in HIV-1 provide better characterization transcript its regulation. Results Initial experiments conducted by standard RT-PCR analysis latently infected J1.1 cell line pNL4.3-transfected 293T cells confirmed HIV-1. A more adapted protocol with limited...
Abstract Modulation of the activation status immune cell populations during pregnancy depends on placental villous cytotrophoblast (VCT) cells and syncytiotrophoblast (STB). Failure in establishment this immunoregulatory function leads to complications. Our laboratory has been studying Syncytin-2 (Syn-2), an endogenous retroviral protein expressed placenta surface exosomes. This plays important role not only STB formation through its fusogenic properties, but also immunosuppressive domain...
Preeclampsia (PE) is characterized by maternal hypertension, proteinuria, oedema and, in 30% of cases, intrauterine growth retardation. Causes are still unknown; however, epidemiological and clinical studies have suggested alterations calcium metabolism. We that PE, transport the syncytiotrophoblast (ST) disturbed. From total placental tissues, we studied expression of: channels (TRPV5, TRPV6 [transient receptor potential vanilloid]), binding proteins (CaBP-9K, CaBP-28K), plasma membrane...
Infection with the human T-cell leukemia virus type 1 (HTLV-1) results in a variety of diseases including adult (ATL), fatal malignancy characterized by uncontrolled proliferation virally infected CD4(+) T cells. The HTLV-1 basic leucine zipper factor (HBZ) is believed to contribute development and maintenance ATL. Unlike other genes, hbz gene encoded on complementary strand provirus therefore not under direct control promoter within 5' long terminal repeat (LTR) provirus. This can undergo...
Human endogenous retroviruses (HERVs) represent approximately 8% of our genome. HERVs influence cellular gene expression and contribute to normal physiological processes such as differentiation morphogenesis. have also been associated with certain pathological conditions, including cancer neurodegenerative diseases. As HTLV-1 causes adult T-cell leukemia HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) has shown modulate host mainly through the powerful Tax transactivator,...
Replication of human immunodeficiency virus type 1 (HIV-1) is increased by different cytokines and T cell activators, also known to modulate tyrosine phosphorylation levels. A novel class protein phosphatase (PTP) inhibitors, peroxovanadium (pV) compounds, were tested for a putative effect on HIV-1 long terminal repeat (LTR) activity. We found that these PTP inhibitors markedly enhanced LTR activity in 1G5 cells, stably transfected line harbors an LTR-driven luciferase construct. direct...
The human T-cell leukemia virus type I (HTLV-I) basic leucine-zipper factor (HBZ) has previously been shown to modulate transcriptional activity of Jun family members. presence a novel isoform HBZ, termed HBZ-SP1, recently characterized in adult (ATL) cells and found be associated with intense nuclear spots. In this study, we investigated the role these bodies regulation JunB.Using fluorescence microscopy, that HBZ-SP1 protein localizes dots corresponding HBZ-NBs nucleoli. We analyzed...
HIV-1 proteins are synthesized from a single transcript in an unspliced form or following splicing, but the existence of antisense protein (ASP) expressed polyadenylated has been suggested. Difficulties linked to detection this mammalian cells led us codon optimize its cDNA. Codon-optimized ASP was indeed efficiently detected various transfected cell lines flow cytometry and confocal microscopy analyses. Western blot analyses also optimized provided evidence instability high multimerization...
Abstract Background Cell-free Human T-cell Leukemia Virus type I (HTLV-I) virions are poorly infectious and cell-to-cell contact is often required to achieve infection. Other factors might thus importantly contribute in increasing infection by HTLV-I. Galectin-1 a galactoside-binding lectin which secreted activated T lymphocytes. Several functions have been attributed this protein including its capacity increase adhesion. Based on previous studies, we postulated that could also accentuate...
Several studies have recently demonstrated the existence of human T-cell leukemia virus type 1 (HTLV-1) antisense transcripts, which allow synthesis newly described HBZ protein. Although previous reports been aimed at understanding potential role protein in HTLV-1 pathogenesis, little is known as to how this viral gene regulated. Here, using our K30-3'asLuc reporter construct, we show that Tax upregulates transcription through its action on TRE sequences located 3' long terminal repeat....