A5062990750- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Viral-associated cancers and disorders
- Evolution and Genetic Dynamics
- Cellular transport and secretion
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- Viral Infections and Vectors
- RNA regulation and disease
- Cutaneous lymphoproliferative disorders research
- Erythrocyte Function and Pathophysiology
- Health Systems, Economic Evaluations, Quality of Life
- Ubiquitin and proteasome pathways
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Phagocytosis and Immune Regulation
- Cancer Research and Treatments
- Cytokine Signaling Pathways and Interactions
- Zoonotic diseases and public health
Centre International de Recherche en Infectiologie
2014-2024
École Normale Supérieure de Lyon
2014-2024
Université Claude Bernard Lyon 1
2014-2024
Inserm
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Fondation pour la Recherche Médicale
2019
La Ligue Contre le Cancer
2013-2018
Virologie et Pathologies Humaines
2009-2012
École Normale Supérieure - PSL
2012
Institut Pasteur
2008-2009
ISG20 is a broad spectrum antiviral protein thought to directly degrade viral RNA. However, this mechanism of inhibition remains controversial. Using the Vesicular Stomatitis Virus (VSV) as model RNA virus, we show here that interferes with replication by decreasing synthesis in absence degradation. Importantly, demonstrate exerts translational control over large panel non-self substrates including those originating from transfected DNA, while sparing endogenous transcripts. This activity...
Nuclear factor (NF)-κB is a major survival pathway engaged by the Human T-Lymphotropic Virus type 1 (HTLV-1) Tax protein. Tax1 activation of NF-κB occurs predominantly in cytoplasm, where binds Essential Modulator (NEMO/IKKγ) and triggers IκB kinases. Several independent studies have shown that Tax1-mediated dependent on ubiquitination. Here, we identify co-immunoprecipitation assays NEMO-Related Protein (NRP/Optineurin) as binding partner for HTLV-1 infected Tax1/NRP co-expressing cells....
Human T lymphotropic Virus type 1 (HTLV-1) is the etiological agent of Adult cell Leukemia/Lymphoma (ATLL) and HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP). Both CD4+ T-cells dendritic cells (DCs) infected with HTLV-1 are found in peripheral blood from carriers. We previously demonstrated that monocyte-derived IL-4 DCs more susceptible to infection than autologous primary T-cells, suggesting DC precedes T-cell infection. However, during transmission, breast-feeding or...
Five to ten million individuals are infected by Human T-cell Leukemia Virus type 1 (HTLV-1). HTLV-1 is transmitted through prolonged breast-feeding, sexual contacts and transmission of T lymphocytes blood transfusion. One percent carriers will develop a severe HTLV-1-associated disease: Adult-T-cell leukemia/lymphoma (ATLL), or neurological disorder named Tropical Spastic Paraparesis/HTLV-1 Associated Myelopathy (TSP/HAM). In vivo, mostly detected in CD4+ T-cells, lesser extent CD8+ cells...
Permanent activation of the NF-κB pathway by human T cell leukemia virus type 1 (HTLV-1) Tax (Tax1) viral transactivator is a key event in process HTLV-1-induced lymphocyte immortalization and leukemogenesis. Although encoding (Tax2) that activates canonical pathway, HTLV-2 does not cause leukemia. These distinct pathological outcomes might be related, at least part, to mechanisms. Tax1 has been shown both ubiquitinated SUMOylated, these two modifications were originally proposed required...
The role of innate immunity in general and type I interferon (IFN-I) particular HTLV-1 pathogenesis is still a matter debate. ADAR1-p150 an Interferon Stimulated Gene (ISG) induced by IFN-I that can edit viral RNAs. We therefore investigated whether it could play the anti-HTLV factor. demonstrate here ADAR1 also expressed absence IFN stimulation activated primary T-lymphocytes are natural target this virus or HTLV-2 chronically infected T-cells. expression increased lymphocytes obtained from...
ABSTRACT Type I interferon (IFN-I) inhibits the replication of different viruses. However, effect IFN-I on human T-lymphotropic virus type 1 (HTLV-1) viral cycle is controversial. Here, we investigated consequences IFN-α addition for steps HTLV-1 and HTLV-2 infection. We first show that alpha (IFN-α) efficiently impairs de novo infection in a T cell line primary lymphocytes. Using pseudotyped viruses expressing envelope, then cell-free insensitive to IFN-α, demonstrating cytokine does not...
Human T-cell lymphotropic virus type 1 (HTLV-1)-induced adult leukemia/lymphoma is an aggressive malignancy. HTLV-2 genetically related to HTLV-1 but does not cause any malignant disease. Tax transactivator (Tax-1) contributes leukemogenesis via NF-κB. We describe transgenic Drosophila models expressing in the compound eye and plasmatocytes. demonstrate that Tax-1 Tax-2 induces ommatidial perturbation increased plasmatocyte proliferation phenotype dependent on Kenny (IKKγ/NEMO), thus...
Abstract The NF-κB pathway is constitutively activated in adult T cell leukemia, an aggressive malignancy caused by Human Leukemia Virus type 1 (HTLV-1). viral oncoprotein Tax triggers this constitutive activation interacting with the ubiquitin-rich IKK complex. We previously demonstrated that Optineurin and TAX1BP1, two members of ubiquitin-binding, Sequestosome-1 (SQSTM-1/p62)-like selective autophagy receptor family, are involved Tax-mediated signaling. Here, using a proximity-dependent...
Human T-cell leukemia viruses type 1 (HTLV-1) and 2 (HTLV-2) share a common genome organization expression strategy but have distinct pathological properties. HTLV-1 is the etiological agent of Adult Leukemia (ATL) HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP), whereas HTLV-2 does not cause hematological disorders only sporadically associated with cases subacute myelopathy. Both HTLV genomes encode two regulatory proteins that play pivotal role in pathogenesis:...
Nipah virus (NiV) is a highly pathogenic emerging bat-borne Henipavirus that has caused numerous outbreaks with public health concerns. It able to inhibit the host innate immune response. Since NF-κB pathway plays crucial role in antiviral response as major transcriptional regulator of inflammation, we postulated its implication still poorly understood NiV immunopathogenesis. We report here inhibits canonical via nonstructural W protein. Translocation protein into nucleus causes nuclear...
Simian T-Leukemia Virus type 1 and Foamy infect non-human primates. While STLV-1, as HTLV-1, causes Adult T-cell Leukemia/lymphoma, SFV infection is asymptomatic. Both retroviruses can be transmitted from NHPs to humans through bites that allow contact between infected saliva recipient blood. Because both viruses CD4+ T-cells, they might interfere with each other replication, this impact viral transmission. Impact of STLV-1 co-infection on replication was analyzed in 18...
The InterFeron-Induced TransMembrane proteins (IFITMs) are members of the dispanin/CD225 family that act as broad viral inhibitors by preventing viral-to-cellular membrane fusion. In this study, we uncover egress from Golgi an important step in biology IFITM3 identifying domain regulates process and similarly controls dispanins IFITM1 PRRT2, protein linked to paroxysmal kinesigenic dyskinesia. case IFITM3, high levels expression wild-type , or mutations domain, lead accumulation drive...
BackgroundKaposi sarcoma-associated herpesvirus (KSHV) is the etiologic agent of primary effusion lymphomas (PEL). PEL cell lines infected with KSHV, but negative for Epstein-Barr virus have a tumorigenic potential in non-obese diabetic/severe combined immunodeficient mice and result efficient engraftment formation malignant ascites notable abdominal distension, consistent clinical manifestations humans. Methodology/Principal FindingsUsing this preclinical mouse model, we demonstrate that...
A transgenic Drosophila melanogaster model to study HTLV-I oncoprotein Tax-driven leukemogenesis in vivo