A5034237013- HIV Research and Treatment
- interferon and immune responses
- Neurobiology and Insect Physiology Research
- Erythrocyte Function and Pathophysiology
- HIV/AIDS drug development and treatment
- Classical Philosophy and Thought
- Hepatitis C virus research
- Hearing, Cochlea, Tinnitus, Genetics
- Historical, Literary, and Cultural Studies
- Circadian rhythm and melatonin
- Hemispheric Asymmetry in Neuroscience
- Parvovirus B19 Infection Studies
- Epigenetics and DNA Methylation
- Blood groups and transfusion
- Virology and Viral Diseases
- Light effects on plants
- Virus-based gene therapy research
- Medical Imaging and Pathology Studies
- Ion channel regulation and function
- Bacteriophages and microbial interactions
- bioluminescence and chemiluminescence research
- Eosinophilic Esophagitis
- Animal Disease Management and Epidemiology
- Advanced biosensing and bioanalysis techniques
- HIV-related health complications and treatments
École Normale Supérieure de Lyon
2014-2024
Centre National de la Recherche Scientifique
2015-2024
Institut de Génomique Fonctionnelle de Lyon
2022-2024
Inserm
2012-2024
Institute for Neurosciences of Montpellier
2023-2024
Université de Montpellier
2023-2024
Max Delbrück Center
2024
Université Claude Bernard Lyon 1
2012-2023
Centre International de Recherche en Infectiologie
2014-2021
Interferon induced transmembrane proteins 1, 2 and 3 (IFITMs) belong to a family of highly related antiviral factors that have been shown interfere with large spectrum viruses including Filoviruses, Coronaviruses, Influenza virus, Dengue virus HIV-1. In all these cases, the reported mechanism inhibition indicates pool IFITM present in target cells blocks incoming viral particles endosomal vesicles where they are subsequently degraded.In this study, we describe an additional through which...
IFITMs are broad antiviral factors that block incoming virions in endosomal vesicles, protecting target cells from infection. In the case of HIV-1, we and others reported existence an additional mechanism through which lead to production reduced infectivity. However, whether this second inhibition is unique HIV or extends other viruses currently unknown. To address question, have analyzed susceptibility a spectrum negative imprinting virion particles infectivity by IFITMs. The results...
ISG20 is a broad spectrum antiviral protein thought to directly degrade viral RNA. However, this mechanism of inhibition remains controversial. Using the Vesicular Stomatitis Virus (VSV) as model RNA virus, we show here that interferes with replication by decreasing synthesis in absence degradation. Importantly, demonstrate exerts translational control over large panel non-self substrates including those originating from transfected DNA, while sparing endogenous transcripts. This activity...
Oral presentations Session 1: Entry & uncoating O1 Host cell polo-like kinases (PLKs) promote early prototype foamy virus (PFV) replication Irena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, Juliane Reh, Tobias Kern, Fabian Lindel, Gesche Gerresheim, Martin Hamann, Erik Müllers, Paul Lesbats, Peter Cherepanov, Serrao, Alan Engelman, Dirk Lindemann O2 A novel entry/uncoating assay reveals the presence of at least two species viral capsids during synchronized HIV-1 infection Claire Da...
To better characterize the behavior of HIV-1 capsids we developed EURT, for Entry/Uncoating assay based on core-packaged RNA availability and Translation. EURT is an alternative to Blam-Vpr, but as reporter translation relies core opening, it can be used study viral behavior. Our reveals existence two major capsid species, a dead end one in which genome readily exposed cytoplasm functional such exposure requires artificial destabilization. Although reverse transcription drives faster loss...
IFITM proteins have been associated with the sequestration of incoming virions in endosomes (target cell protection) and production virion particles that incorporate IFITMs exhibit decreased infectivity (negative imprinting infectivity). How latter is regulated whether these two antiviral properties are related remain unknown. By examining behavior a large panel IFITM3 mutants against HIV-1, we determined essentially packaged into proportionally to their intracellular levels expression....
HIV-1, an enveloped RNA virus, produces viral particles that are known to be much more heterogeneous in size than is typical of non-enveloped viruses. We present here a novel strategy study HIV-1 Viral Like Particles (VLP) assembly by measuring the distribution these purified VLPs and subsequent cores thanks Atomic Force Microscopy imaging statistical analysis. This allowed us identify whether presence acts as modulator for heterogeneity large population particles. These results analyzed...
Touch sensation is primarily encoded by mechanoreceptors, called low-threshold mechanoreceptors (LTMRs), with their cell bodies in the dorsal root ganglia. Because of great diversity terms molecular signature, terminal endings morphology, and electrophysiological properties, mirroring complexity tactile experience, LTMRs are a model choice to study cues differentially controlling neuronal diversification. While transcriptional codes that define different LTMR subtypes have been extensively...
IFITMs are broad viral inhibitors capable of interfering with both early and late phases the replicative cycle many different viruses. By comparing 21 IFITM proteins issued from animal species for their ability to inhibit HIV-1, we have identified several that exhibit either enhanced or impaired antiviral behavior.
Abstract Touch sensation is primarily encoded by mechanoreceptors, called Low-Threshold Mechanoreceptors (LTMRs), with their cell bodies in the Dorsal Root Ganglia (DRG). Because of great diversity terms molecular signature, terminal endings morphology and electrophysiological properties, mirroring complexity tactile experience, LTMRs are a model choice to study cues differentially controlling neuronal diversification. While transcriptional codes that define different LTMR subtypes have been...
Touch sensation is primarily encoded by mechanoreceptors, called low-threshold mechanoreceptors (LTMRs), with their cell bodies in the dorsal root ganglia. Because of great diversity terms molecular signature, terminal endings morphology, and electrophysiological properties, mirroring complexity tactile experience, LTMRs are a model choice to study cues differentially controlling neuronal diversification. While transcriptional codes that define different LTMR subtypes have been extensively...
Touch sensation is primarily encoded by mechanoreceptors, called Low-Threshold Mechanoreceptors (LTMRs), with their cell bodies in the Dorsal Root Ganglia (DRG). Because of great diversity terms molecular signature, terminal endings morphology and electrophysiological properties, mirroring complexity tactile experience, LTMRs are a model choice to study cues differentially controlling neuronal diversification. While transcriptional codes that define different LTMR subtypes have been...