A5100428638- Mosquito-borne diseases and control
- Bone fractures and treatments
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Viral Infections and Vectors
- Antibiotic Resistance in Bacteria
- HIV Research and Treatment
- Respiratory viral infections research
- Influenza Virus Research Studies
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Hip and Femur Fractures
- interferon and immune responses
- Immunotherapy and Immune Responses
- Vector-borne infectious diseases
- Monoclonal and Polyclonal Antibodies Research
- Malaria Research and Control
- Phenothiazines and Benzothiazines Synthesis and Activities
- RNA modifications and cancer
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- Toxin Mechanisms and Immunotoxins
- Identification and Quantification in Food
Affiliated Hospital of Southwest Medical University
2021-2025
Shandong Center for Disease Control and Prevention
2008-2025
Zhuhai Institute of Advanced Technology
2023-2025
Chongqing Medical University
2022-2025
Second Affiliated Hospital of Chongqing Medical University
2022-2025
Affiliated Hospital of North Sichuan Medical College
2025
Dalian Medical University
2022-2025
Soochow University
2008-2024
University of Arizona
2021-2024
East China University of Science and Technology
2009-2024
The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied mice lacking these key enzymes. PI3Kγ was required for chemoattractant-induced production phosphatidylinositol 3,4,5-trisphosphate [PtdIns (3,4,5)P 3 ] has an important role superoxide chemotaxis mouse neutrophils T cell–independent antigen-specific antibodies composed the immunoglobulin λ light chain (TI-Igλ L ). study PLC-β2 -β3 revealed that PLC pathways have...
Patients with human severe combined immunodeficiency (SCID) can be divided into those B lymphocytes (B + SCID) and without − SCID). Although several genetic causes are known for SCID, the etiology of SCID has not been defined. Six 14 patients tested were found to carry a mutation recombinase activating gene 1 ( RAG-1 ), RAG-2 , or both. This resulted in functional inability form antigen receptors through recombination links defect one site-specific systems disease.
Knowledge about the world phylogeny of human mitochondrial DNA (mtDNA) is essential not only for evaluating pathogenic role specific mtDNA mutations but also performing reliable association studies between haplogroups and complex disorders. In past few years, main features East Asian portion have been determined on basis complete sequencing efforts, representatives several basal lineages were still lacking. Moreover, some recently published sequences did apparently fit into known...
ABSTRACT The plus-strand RNA genome of flavivirus contains a 5′ terminal cap 1 structure (m 7 GpppAmG). flaviviruses encode one methyltransferase, located at the N-terminal portion NS5 protein, to catalyze both guanine N-7 and ribose 2′-OH methylations during viral formation. Representative methyltransferases from dengue, yellow fever, West Nile virus (WNV) sequentially generate GpppA → m GpppAm. 2′-O methylation can be uncoupled methylation, since GpppA-RNA readily methylated GpppAm-RNA....
Cancer stem cells (CSCs) or tumor initiating (TICs) contribute to tumorigenesis, metastasis, recurrence and chemoresistance. CD133, a pentaspan membrane glycoprotein, has been used as cell biomarker for isolation of stem-like from variety normal pathological tissues well lines since its discovery in 1999. Recent studies are focusing on the functionality CD133. In this review, we summarize new insights into CD133 regulation involvement self-renewal, resistance, metabolism, differentiation,...
Confocal imaging and time-lapsed videomicroscopy were used to study the directionality, motility, rate of cell movement, morphologies phosphoinositide 3-kinase γ ( PI3K )γ −/− neutrophils undergoing chemotaxis in Zigmond chambers containing N -formyl-Met-Leu-Phe gradients. Most failed translocate up cheomotactic gradient. A partial reduction motility abnormal also observed. In wild-type neutrophils, pleckstrin homology domain-containing protein kinase B (AKT) F-actin colocalize leading edge...
Otitis media, a common and often recurrent bacterial infection of childhood, is major reason for physician visits the prescription antimicrobials. Haemophilus influenzae cause approximately 20% episodes otitis but most strains lack capsule, factor known to play critical role in virulence causing invasive H. disease. Here we show that capsule-deficient (nontypeable) strains, sialic acid, terminal residue core sugars lipopolysaccharide (LPS), pathogenesis experimental media chinchillas. We...
Significance We provide a de novo genome of the Tibetan frog, Nanorana parkeri , and conduct series comparisons with other vertebrates. Approximately one-half consists transposable elements (TEs). The frequencies distributional patterns TEs differ considerably between Xenopus only amphibian for which has been sequenced. genomes these two frogs exhibit substantial homologous synteny blocks rare interchromosomal intrachromosomal rearrangements. also identify 11 Mb amphibian-specific conserved...
The flavivirus genome encodes a single polyprotein precursor requiring multiple cleavages by host and viral proteases in order to produce the individual proteins that constitute an infectious virion. Previous studies have revealed NS2B cofactor of NS2B-NS3 heterocomplex protease displays conformational dynamic between active inactive states. Here, we developed switch assay based on split luciferase complementation (SLC) monitor change characterize candidate allosteric inhibitors. Binding...
Hepatitis B virus X (HBx) protein is implicated in hepatitis (HBV)–associated liver carcinogenesis. However, it remains unclear whether HBx–expressing hepatic progenitor cells (HPCs) are attributed to tumor formation. In this study, by using HBx transgenic mice and a 3,5–diethoxycarbonyl–1,4–dihydrocollidine (DDC)–induced injury model, the relationship between expression tumorigenicity of HPCs was analyzed. Compared with control mice, an elevated number EpCAM+ characteristics observed after...
The snub-nosed monkey genus Rhinopithecus includes five closely related species distributed across altitudinal gradients from 800 to 4,500 m. bieti, roxellana, and strykeri inhabit high-altitude habitats, whereas brelichi avunculus lowland regions. We report the de novo whole-genome sequence of R. bieti genomic sequences for four other species. Eight shared substitutions were found in six genes lung function, DNA repair, angiogenesis monkeys. Functional assays showed that variant CDT1...
Abstract Background Cyclosporine A (CsA) is routinely used to treat patients with steroid‐refractory acute severe ulcerative colitis (ASUC). Here, we studied the underlying mechanisms of CsA‐mediated alleviation in ASUC patients. Methods Neutrophil functions including expression cytokines, apoptosis, and migration were measured by qRT‐PCR, flow cytometry, Transwell assay. Dynamic changes glycolysis tricarboxylic acid (TCA) cycle a Seahorse extracellular flux analyzer. Gene differences...
The mechanism by which the high-bone-mass (HBM) mutation (G171V) of Wnt coreceptor LRP5 regulates canonical signaling was investigated. previously shown to reduce DKK1-mediated antagonism, suggesting that first YWTD repeat domain where G171 is located may be responsible for DKK-mediated antagonism. However, we found third repeat, but not domain, required Instead, G171V disrupted interaction with Mesd, a chaperone protein LRP5/6 transport coreceptors cell surfaces, resulting in fewer...
Toxoplasma gondii tachyzoites were loaded with the fluorescent indicator fura 2 to investigate transport mechanisms involved in maintaining their intracellular Ca2+ homoeostasis. The mitochondrial ATPase inhibitor oligomycin and endoplasmic-reticulum Ca2+-ATPase thapsigargin increased concentration ([Ca2+]i), thus indicating requirement for ATP involvement of endoplasmic reticulum effect was more accentuated presence extracellular Ca2+, clearly showing that, as occurs other eukaryotic cells,...
The vacuolar-type proton-translocating pyrophosphatase (V-H+-PPase) is an enzyme previously described in detail only plants. This paper demonstrates its presence the trypanosomatid Trypanosoma cruzi. Pyrophosphate promoted organellar acidification permeabilized amastigotes, epimastigotes, and trypomastigotes of T. activity was stimulated by K+ ions inhibited Na+ pyrophosphate analogs, as plant activity. Separation epimastigote extracts on Percoll gradients yielded a dense fraction that...
The binding of tumor necrosis factor alpha (TNF-alpha) to the type-1 TNF receptor (TNFRc1) plays an important role in inflammation. Despite clinical success biologics (antibodies, soluble receptors) for treating TNF-based autoimmune conditions, no potent small molecule antagonists have been developed. Our screening chemical libraries revealed that N-alkyl 5-arylidene-2-thioxo-1,3-thiazolidin-4-ones were this protein-protein interaction. After optimization, we discovered IW927, which potently...
In order to achieve a thorough coverage of the basal lineages in Chinese matrilineal pool, we have sequenced mitochondrial DNA (mtDNA) control region and partial coding segments 6,093 mtDNAs sampled from 84 populations across China. By comparing with available complete mtDNA sequences, 194 those could not be firmly assigned into haplogroups. Completely sequencing 51 representatives selected these unclassified identified number novel lineages, including five haplogroups that directly emanate...