A5103006331- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Pain Mechanisms and Treatments
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Developmental Biology and Gene Regulation
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Erythrocyte Function and Pathophysiology
- Retinal Development and Disorders
- Autism Spectrum Disorder Research
- Wnt/β-catenin signaling in development and cancer
- Hearing, Cochlea, Tinnitus, Genetics
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Classical Philosophy and Thought
- RNA Research and Splicing
- Cellular transport and secretion
- Neurotransmitter Receptor Influence on Behavior
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Signaling Pathways in Disease
- Neurogenetic and Muscular Disorders Research
- Neuroscience and Neural Engineering
Institute for Neurosciences of Montpellier
2012-2025
Inserm
2012-2024
Université de Montpellier
2003-2024
École Normale Supérieure de Lyon
2023-2024
Centre National de la Recherche Scientifique
1998-2024
Institut de Génomique Fonctionnelle de Lyon
2023-2024
Hôpital Saint Eloi
2004-2019
Pioneer (Japan)
2015
Institut National de Recherche en Santé Publique
2009
Laboratoire de Génétique Médicale
2002
Brain-derived neurotrophic factor (BDNF), a member of the nerve growth (NGF) gene family, has been shown to influence survival and differentiation specific classes neurons in vitro vivo. The possibility that neurotrophins are also involved processes neuronal plasticity only recently begun receive attention. To determine whether BDNF function such as long-term potentiation (LTP), we produced strain mice with deletion coding sequence gene. We then used hippocampal slices from these investigate...
Long-term potentiation (LTP) has been shown to be impaired in mice deficient the brain-derived neurotrophic factor (BDNF) gene, as well a number of other knockout animals. Despite its power gene-targeting approach is always fraught with danger looking at cumulative direct and indirect effects absence particular gene rather than immediate function. The re-expression specific selective time point site gene-defective mutants presents potent procedure overcome this limitation evaluate causal...
Telling Sandpaper from Satin Pacinian corpuscles are mechano-receptors tuned to detect high-frequency, low-amplitude, signals. Found in human palm and fingertips, they useful for discrimination of rough smooth textures, a sensitivity seemingly amplified by the ridges fingerprints. Wende et al. (p. 1373 , published online 16 February) identified mutation humans that disrupts this texture, but leaves other facets touch, such as tactile spatial acuity, intact.
Abstract In contrast to sympathetic and sensory neurons in the peripheral nervous system, neurotrophic requirements for central system (CNS) have not been clearly identified. The inactivation of specific factors their receptors by gene targeting has shown that there are no major changes neuron numbers CNS. This suggests an overlap between action different brain during development. Here we studied survival hippocampal prepared from embryonic rats, using culture conditions. Whereas survive...
Peripheral neuropathic pain (PNP) is a debilitating and intractable chronic disease, for which sensitization of somatosensory neurons present in dorsal root ganglia that project to the spinal cord key physiopathological process. Here, we show hematopoietic cells at nerve injury site express cytokine FL, ligand fms-like tyrosine kinase 3 receptor (FLT3). FLT3 activation by intra-sciatic injection FL sufficient produce hypersensitivity, activate PNP-associated gene expression generate...
Dopaminergic cells in the retina express receptor for brain-derived neurotrophic factor (BDNF) (Cellerino and Kohler, 1997). To investigate whether BDNF can influence development of retinal dopaminergic pathway, we performed intraocular injections during second or third postnatal week visualized system with tyrosine hydroxylase (TH) immunohistochemistry. Both regimens treatment caused an increase TH immunoreactivity stratum 1 3 inner plexiform layer (IPL). D2 dopamine immunoreactivity, a...
The reorganization of specific neuronal connections is a typical feature the developing nervous system. It assumed that refinement in sensory systems requires spontaneous activity before onset cochlear function and selective experience during ensuing period. mechanism through currently postulated as being based on reinforcement active projections by neurotrophins. We studied presumed role neurotrophins for rearrangement afferent efferent fibers precisely innervated auditory end organ,...
The Escherichia coli ada-alkB operon encodes a 39-kDa protein (Ada) that is DNA-repair methyltransferase and 27-kDa (AlkB) of unknown function. By DNA blot hybridization analysis we show the alkylation-sensitive E. mutant BS23 [Sedgwick, B. & Lindahl, T. (1982) J. Mol. Biol. 154, 169-175] deletion lacking entire operon. Despite absence ada gene its product, cells contain detectable levels activity. We conclude in product other than ada. A similar activity was detected extracts an...
Escherichia coli can ameliorate the toxic effects of alkylating agents either by preventing DNA alkylation or repairing damage. The alkylation-sensitive phenotype E. alkB mutants marks pathway as an extremely effective defense mechanism against cytotoxic SN2, but not SN1, agents. Although it is clear that AlkB helps cells to better handle alkylated DNA, no repair function could be assigned purified protein, suggesting acts part a complex regulate expression other genes whose products are...
Different subsets of dorsal root ganglion (DRG) mechanoreceptors transduce low- and high-intensity mechanical stimuli. It was shown recently that, in vivo , neurotrophin-4 (NT-4)-dependent D-hair specifically express a voltage-activated T-type calcium channel (Ca v 3.2) that may be required for their mechanoreceptive function. Here we show can identified vitro by rosette-like morphology the presence NT-4 these rosette neurons are almost all absent DRG cultures taken from knock-out mice. In...
We investigated the molecular determinants of Ca 2+ -activated chloride current (CaCC) expressed in adult sensory neurons after a nerve injury. Dorsal root ganglia express transcripts three gene families known to induce CaCCs heterologous systems: bestrophin, tweety, and TMEM16. found with quantitative transcriptional analysis situ hybridization that injury induced upregulation solely bestrophin-1 neurons. Gene screening RNA interference single demonstrated mouse Best1 is required for...
Distinct subfamilies of proneural bHLH transcription factors (TFs) control neurogenesis and influence neuronal fate specification throughout the developing nervous system. While members different generally display divergent expression functions, those within same subfamily are often co-expressed, raising questions about their respective roles. Here, we assess functional equivalence two paralogous TFs Neurog1 Neurog2, focusing on neurons dorsal root ganglia (DRG) which underlie our ability to...