A5091246104- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Spinal Cord Injury Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Osteoarthritis Treatment and Mechanisms
- Pain Mechanisms and Treatments
- Photoreceptor and optogenetics research
- Cancer-related molecular mechanisms research
- Nerve injury and regeneration
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Spectroscopy Techniques in Biomedical and Chemical Research
- Hearing, Cochlea, Tinnitus, Genetics
- Ocular Disorders and Treatments
- Corneal surgery and disorders
- Advanced Fluorescence Microscopy Techniques
- Memory and Neural Mechanisms
- Advanced biosensing and bioanalysis techniques
- Neonatal Respiratory Health Research
- Photoacoustic and Ultrasonic Imaging
- Neuroscience and Neural Engineering
- Pluripotent Stem Cells Research
- Lipid Membrane Structure and Behavior
Institute for Neurosciences of Montpellier
2008-2024
Inserm
2011-2024
Université de Montpellier
2007-2024
Hôpital Saint Eloi
2004-2023
Institut des Cellules Souches pour le Traitement et l'Étude des Maladies Monogéniques
2022
Servier (France)
2020
Institut des Biomolécules Max Mousseron
2018
Abstract Mitochondrial activity is central to tissue homeostasis. Mitochondria dysfunction constitutes a hallmark of many genetic diseases and plays key role in tumor progression. The essential mitochondria, added their recently documented capacity transfer from cell cell, obviously contributes current interest. However, determining the proper mitochondria defined biological contexts was hampered by lack suitable experimental tools. We designed protocol (MitoCeption) directly quantitatively...
Eukaryotic cells harbor a small multiploid mitochondrial genome, organized in nucleoids spread within the network. Maintenance and distribution of DNA (mtDNA) are essential for energy metabolism, lineage primordial germ cells, to prevent mtDNA instability, which leads many debilitating human diseases. Mounting evidence suggests that actors network dynamics, among is intramitochondrial dynamin OPA1, might be involved these processes. Here, using siRNAs specific OPA1 alternate spliced exons,...
The etiology of benign paroxysmal positional vertigo (BPPV) remains obscure in many cases and women are affected more often than men. A recent prospective study, performed >50 years age suffering from recurrent BPPV, showed associated osteopenia or osteoporosis a large percentage these patients. These results suggested the possible relationship between BPPV decreased fixation calcium bone years. To test this hypothesis, an experimental study was adult female rats. Utricular otoconia rats...
Cell penetrating peptides constitute a potent approach to overcome the limitations of in vivo siRNA delivery. We recently proposed peptide-based nanoparticle system, CADY, for efficient delivery into numerous cell lines. CADY is secondary amphipathic peptide that forms stable complexes with thereby improving both their cellular uptake and biological response. With aim understanding mechanism CADY:siRNA complexes, we have combined biochemical, confocal electron microscopy approaches. In...
No curative treatment is available for any deficits induced by spinal cord injury (SCI).Following injury, microglia undergo highly diverse activation processes, including proliferation, and play a critical role on functional recovery.In translational objective, we investigated whether transient pharmacological reduction of proliferation after beneficial recovery SCI in mice nonhuman primates.Methods: The colony stimulating factor-1 receptor (CSF1R) regulates differentiation, survival...
Whole cell patch-clamp recordings of calcium-activated chloride current [ I Cl(Ca) ] were made from adult sensory neurons naive and axotomized mouse L 4 –L 6 lumbar dorsal root ganglia after 1 day culture in vitro. A basal was specifically expressed a subset medium-diameter (30–40 μm). Prior nerve injury, induced by sciatic transection 5 days before experiments, increased both amplitude its expression neurons. Moreover, injury also new subpopulation neurons, the large-diameter (40–50...
CCR6 is a chemokine receptor that expressed at the cell surface of Th17 cells, an IL-17- and IL-22-secreting population CD4(+) T cells with antipathogenic, as well inflammatory, properties. In current study, we have determined involvement in human lymphocyte migration toward inflamed tissue by analyzing capacity its ligands to induce arrest these onto endothelium vitro under flow conditions. We show polarized, situ-differentiated, skin-derived clones activated via TCR-CD3 complex produce...
Neurons have intrinsic capability to regenerate after lesion, though not spontaneously. Spinal cord injury (SCI) causes permanent neurological impairments partly due formation of a glial scar that is composed astrocytes and microglia. Astrocytes play both beneficial detrimental roles on axonal re-growth, however, their precise role SCI currently under debate.We analyzed molecular changes in at multiple stages two severities using cell-specific transcriptomic analyses.We demonstrate astrocyte...
Human-induced pluripotent stem cell-derived retinal organoids are a valuable tool for disease modelling and therapeutic development. Many efforts have been made over the last decade to optimise protocols generation of that correctly mimic human retina. Most use common media supplements; however, protocol-dependent variability impacts data interpretation. To date, lack systematic comparison given protocol with or without supplements makes it difficult determine how they influence...
Retinitis pigmentosa (RP) is the most common inherited retinal disease (IRD) and characterized by photoreceptor degeneration progressive vision loss. We report 4 patients presenting with RP from 3 unrelated families variants in TBC1D32, which to date has never been associated an IRD. To validate TBC1D32 as a putative causative gene, we combined Xenopus vivo approaches human induced pluripotent stem cell-derived (iPSC-derived) models. Our data showed that was expressed during development it...
Different subsets of dorsal root ganglion (DRG) mechanoreceptors transduce low- and high-intensity mechanical stimuli. It was shown recently that, in vivo , neurotrophin-4 (NT-4)-dependent D-hair specifically express a voltage-activated T-type calcium channel (Ca v 3.2) that may be required for their mechanoreceptive function. Here we show can identified vitro by rosette-like morphology the presence NT-4 these rosette neurons are almost all absent DRG cultures taken from knock-out mice. In...
Glioblastoma Multiforme (GBM) invasiveness renders complete surgical resection impossible and highly invasive Initiating Cells (GICs) are responsible for tumour recurrence. Their dissemination occurs along pre-existing fibrillary brain structures comprising the aligned myelinated fibres of corpus callosum (CC) laminin (LN)-rich basal lamina blood vessels. The extracellular matrix (ECM) these environments regulates GIC migration, but underlying mechanisms remain largely unknown. In order to...
Abstract CDK5 plays a major role in neuronal functions, and is hyperactivated neurodegenerative pathologies as well glioblastoma neuroblastoma. Although this kinase constitutes an established biomarker pharmacological target, there are few means of probing its activity cell extracts or living cells. To aim fluorescent peptide reporter activity, derived from library CDK5‐specific substrates, engineered ability to respond recombinant CDK5/p25 reported on through sensitive changes fluorescence...
Background: Chronic Obstructive Pulmonary Disease (COPD), a major cause of mortality and disability, is complex disease with heterogeneous ill-understood biological mechanisms. Human induced pluripotent stem cells (hiPSCs) are promising tool to model human disease, including the impact genetic susceptibility. Methods: We developed simple reliable method for reprogramming peripheral blood mononuclear into hiPSCs differentiate them air–liquid interface bronchial epithelium within 45 days....
Myelin sheath produced by Schwann cells covers and nurtures axons to speed up nerve conduction in peripheral nerves. Demyelinating neuropathies result from the loss of this myelin so far, no treatment exists prevent cell demyelination. One major hurdle design a therapy for demyelination is lack reliable measures evaluate outcome on patients but also living animal models. Non‐linear microscopy techniques which include second harmonic generation (SHG), third (THG) coherent anti‐stokes Raman...
Neurotrophin-3 (NT-3), its cognate receptor trkC, and voltage-gated calcium channels are coexpressed by embryonic pyramidal neurons before target contact, but their functions at this stage of development still unclear. We show here that, in vitro , anti-NT-3 anti-trkC antibodies blocked the increase, NT-3 reversed decrease number calbindin-D 28k -positive induced by, respectively, channel activations blockades. Similar results were obtained with single-neuron microcultures. In addition,...
The effect of a 645 nm Light Emitting Diode (LED) light irradiation on the neurite growth velocity adult Dorsal Root Ganglion (DRG) neurons with peripheral axon injury 4–10 days before plating and without previous was investigated. real amount reaching calculated by taking into account optical characteristics source media in path. knowledge these parameters is essential to be able compare results literature way reduce inconsistencies. We found that 4 min mean irradiance 11.3 mW/cm2...
Traumatic spinal cord injury (SCI) induces irreversible autonomic and sensory-motor impairments. A large number of patients exhibit chronic SCI no curative treatment is currently available. Microglia are predominant immune players after SCI, they undergo highly dynamic processes, including proliferation morphological modification. In a translational aim, we investigated whether microglia persists at stage hemisection brief pharmacological could modulate microglial responses. We first carried...
Central nervous system (CNS) injury has been observed to lead microglia activation and monocytes infiltration at the lesion site. Ex vivo diffusion magnetic resonance imaging (diffusion MRI or DWI) allows detailed examination of CNS tissues, recent advances in clearing procedures allow fluorescent-labeled cells high resolution. No study yet combined ex establish a possible link between microglia/monocytes response coefficient context spinal cord (SCI). We carried out different time-points...
Laser scanning microscopes combining a femtosecond Ti:sapphire laser and an optical parametric oscillator (OPO) to duplicate the line have become available for biologists. These systems are primarily designed multi-channel two-photon fluorescence microscopy. However, without any modification, complementary non-linear microscopy such as second-harmonic generation (SHG) or third harmonic (THG) can also be performed with this set-up, allowing label-free imaging of structured molecules aqueous...
Abstract Cultured immature hippocampal neurons from embryonic 17‐day‐old rats were used to explore activity‐dependent regulation of neuronal phenotype differentiation in the developing hippocampus. The calbindin‐D 28k pyramidal appeared during first 6 days culture, and was expressed by 12% cells on day 6. Daily stimulation with 50 m KCl 5 vitro increased number ‐positive without affecting survival. This effect prevented buffering extracellular Ca 2+ . Omega‐agatoxin‐IVA‐sensitive Q‐type...