A5029615414- Retinal Development and Disorders
- Neuropeptides and Animal Physiology
- Retinal Diseases and Treatments
- Receptor Mechanisms and Signaling
- Retinoids in leukemia and cellular processes
- Ubiquitin and proteasome pathways
- Hypothalamic control of reproductive hormones
- Mitochondrial Function and Pathology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Photoreceptor and optogenetics research
- Monoclonal and Polyclonal Antibodies Research
- Neuroendocrine regulation and behavior
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Ion channel regulation and function
- Antioxidant Activity and Oxidative Stress
- Photochromic and Fluorescence Chemistry
- RNA regulation and disease
- Glaucoma and retinal disorders
- Peptidase Inhibition and Analysis
- Circadian rhythm and melatonin
- interferon and immune responses
- T-cell and B-cell Immunology
- Retinopathy of Prematurity Studies
- Neuroscience and Neuropharmacology Research
Inserm
2015-2024
Université de Montpellier
2004-2024
Institute for Neurosciences of Montpellier
2014-2024
Hôpital Saint Eloi
2005-2019
Institute of Cell Biology
2015
Centre de Référence Déficits Immunitaires Héréditaires
2004-2005
University of Puerto Rico at Carolina
2003
Université Toulouse III - Paul Sabatier
2003
Centre National de la Recherche Scientifique
1988-2002
Universidad de Alcalá
2002
Pituitary adenylate cyclase-activating polypeptide (PACAP)-27 and PACAP-38 are neuropeptides of the vasoactive intestinal peptide/secretin/glucagon family. We previously described alternative splicing region encoding third intracellular loop PACAP receptor generating six isoforms with differential signal transduction properties (Spengler, D., Waeber, C., Pantaloni, Holsboer, F., Bockaert, J., Seeburg, P. H., Journot, L. (1993) Nature 365, 170-175). In addition, we demonstrated that potencies...
The G protein o is highly expressed in neurons and mediates effects of a group rhodopsin-like receptors that includes the opioid, α 2 -adrenergic, M2 muscarinic, somatostatin receptors. In vitro , also activated by growth cone-associated M r 43,000 (GAP43) Alzheimer amyloid precursor protein, but it not known whether this occurs intact cells. To learn about roles may play cells whole body homeostasis, we disrupted gene encoding subunits embryonic stem derived -deficient mice. Mice with (α...
Cisplatin (CDDP) is a highly effective chemotherapeutic agent but with significant ototoxic side effects. Apoptosis an important mechanism of cochlear hair cell loss following exposure to level CDDP. This study examines intracellular pathways involved in death induced by CDDP vivo develop therapeutic strategies protect the auditory receptor from CDDP-initiated hearing loss. Guinea pigs were treated systemic administration Cochlear cells CDDP-treated animals exhibited classic apoptotic...
Pituitary adenylate cyclase–activating polypeptide (PACAP) is a ubiquitous neuropeptide of the vasoactive intestinal peptide (VIP) family that potentiates glucose-stimulated insulin secretion. Pancreatic β cells express two PACAP receptor subtypes, PACAP-preferring (PAC1) and VIP-shared (VPAC2) receptor. We have applied gene targeting approach to create mouse lacking PAC1 (PAC1–/–). These mice were viable normoglycemic, but exhibited slight feeding hyperinsulinemia. In vitro, in isolated...
The circadian clock located in the suprachiasmatic nucleus (SCN) organizes autonomic and behavioral rhythms into a near 24 hr time that is adjusted daily to solar cycle via direct projection from retina, retinohypothalamic tract (RHT). This neuronal pathway costores neurotransmitters PACAP glutamate, which seem be important for light-induced resetting of clock. At molecular level genes mPer1 mPer2 are believed target light signaling In this study, we investigated possible role PACAP-type 1...
Dominant optic atrophy is a rare inherited nerve degeneration caused by mutations in the mitochondrial fusion gene OPA1. Recently, clinical spectrum of dominant has been extended to frequent syndromic forms, exhibiting various degrees neurological and muscle impairments frequently found diseases. Although characterized specific loss retinal ganglion cells, pathophysiology still poorly understood. We generated an Opa1 mouse model carrying recurrent Opa1(delTTAG) mutation, which 30% all...
Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are two mediators synthesized by immune cells, specially under inflammatory antigen stimulation conditions. Reports have shown that neuropeptides attenuate the deleterious consequences of septic shock both down-regulating production proinflammatory stimulating anti-inflammatory cytokines activated macrophages. In this study, we used a knockout for PACAP receptor (PAC1 −/− ) to demonstrate an...
Increasing evidence suggests that dysregulation of lipid metabolism is associated with neurodegeneration in retinal diseases such as age-related macular degeneration and brain disorders Alzheimer’s Parkinson’s diseases. Lipid storage organelles (lipid droplets, LDs), accumulate many cell types response to stress, it now clear LDs function not only stores but also dynamic regulators the stress response. However, whether these are always protective or can be deleterious unknown. Here, we...
The olfactory bulb plays a critical role in odor discrimination and processing cues controlling social behavior mammals. Given that the pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1) is highly expressed bulb, we examined its regulating olfaction investigation. We found detection of nonsocial stimuli was similar PAC1-deficient mice wild-type (WT) littermates. In contrast, displayed markedly abnormal behaviors. exhibited faster decrease investigation after...
Bovine brain contains two GTP-binding proteins, Gi and Go, which are substrates for ADP ribosylation by pertussis toxin. The protein mediates hormone GTP inhibition of adenylate cyclase, but the function precise tissue distribution Go unknown. To immunologically probe localization we have purified alpha G beta, gamma subunits raised antibodies against them. polyclonal anti-Go obtained were very selective compared to or Gs alpha. positive immunoreactivities investigated in different tissues...
Mutations in the mitochondrial dynamin-related GTPase OPA1 cause autosomal dominant optic atrophy (ADOA), but pathophysiology of this disease is unknown. As a first step functional studies, study was conducted to evaluate expression Opa1 whole retina and isolated retinal ganglion cells (RGCs) test effects downregulation cultured RGCs.Opa1 mRNA isoforms from total RGCs freshly by immunopanning were determined RT-PCR. Protein examined immunohistochemistry Western blot with antibodies against...
AbstractIn the cochlea, mammalian auditory organ, fibrocytes of mesenchymal nonsensory regions play important roles in cochlear physiology, including maintenance ionic and hydric components endolymph. Occurrence human deafness fibrocyte alterations underlines their critical function. We recently described a novel gene, Otos, which encodes otospiralin, small protein unknown function that is produced by cochlea vestibule. now have generated mice with deletion Otos found they show moderate...
We have localized the guanine nucleotide-binding protein, Go, in primary cultures of pure neuronal and glial cells prepared from different mouse brain areas. Immunoblotting experiments with selective affinity-purified polyclonal rabbit antibodies to 39 kDa alpha subunit Go (Go alpha) indicated that is distributed both neurons cells. accounts for 0.3% total membrane proteins striatal neurons. High specific immunoreactivity was also detected cortical cerebellar granule Similarly, contain large...
The retinohypothalamic tract (RHT) is a retinofugal neuronal pathway which, in mammals, mediates nonimage-forming vision to various areas the brain involved circadian timing, masking behavior, and regulation of pupillary light reflex. RHT costores two neurotransmitters glutamate pituitary adenylate cyclase activating peptide (PACAP), which rather complex interplay are mediators photic adjustment system. To further characterize role PACAP/PACAP receptor type 1 (PAC1) signaling entrainment...
Age-related macular degeneration (AMD) is a multifactorial pathology and its progression exacerbated by oxidative stress. Oxidation photo-oxidation reactions modify lipids in retinal cells, contribute to tissue injury, lead the formation of toxic adducts. In particular, autofluorescent pigments such as N-retinylidene-N-retinylethanolamine (A2E) accumulate lipofuscin pigment epithelial production additional reactive oxygen species (ROS), cell degeneration. an effort develop efficient...
We report here that PAC1 KO females display decreased fertility, whereas male fertility was normal. ICC on pituitary section showed FSH, LH, and prolactin synthesis were not affected in mice. Moreover, the pituitary-gonadal axis responded properly to an acute fasting test Hence, phenotype of null mice provides clear evidence for role receptor reproduction process.