A5017167843- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Hypothalamic control of reproductive hormones
- RNA Research and Splicing
- RNA modifications and cancer
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- RNA and protein synthesis mechanisms
- Developmental Biology and Gene Regulation
- Nerve injury and regeneration
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Protein Hydrolysis and Bioactive Peptides
- Axon Guidance and Neuronal Signaling
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fibroblast Growth Factor Research
- Congenital heart defects research
- Diabetes Treatment and Management
- Pluripotent Stem Cells Research
- Protease and Inhibitor Mechanisms
Centre National de la Recherche Scientifique
2000-2022
Université Paris-Saclay
2008-2022
Institut des Neurosciences Paris-Saclay
2015-2022
Complexité, Innovation et Activités Motrices et Sportives
2022
Université Paris-Sud
2013-2018
Institut National de la Recherche Agronomique
2013
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2007-2011
University College London
2003-2010
Wolfson Foundation
2003-2008
Institute for Neurosciences of Montpellier
2003
The adult mammalian brain and spinal cord contain glial precursors that express platelet-derived growth factor receptor α subunit (PDGFRA) the NG2 proteoglycan. These “NG2 cells” descend from oligodendrocyte in perinatal CNS continue to generate myelinating oligodendrocytes gray white matter of postnatal brain. It has been proposed cells can also reactive astrocytes at sites injury or demyelination. To test this we examined fates PDGFRA/NG2 mouse during experimental autoimmune...
Oligodendrocyte precursors (OLPs or 'NG2 cells') are abundant in the adult mouse brain, where they continue to proliferate and generate new myelinating oligodendrocytes. By cumulative BrdU labelling, we estimated cell cycle time TC proportion of NG2 cells that is actively cycling (the growth fraction) at approximately postnatal day 6 (P6), P60, P240 P540. In corpus callosum, increased from <2 days P6 9 P60 70 cortex, 2 >150 over same period. The fraction remained relatively invariant 50%...
Pituitary adenylate cyclase–activating polypeptide (PACAP) is a ubiquitous neuropeptide of the vasoactive intestinal peptide (VIP) family that potentiates glucose-stimulated insulin secretion. Pancreatic β cells express two PACAP receptor subtypes, PACAP-preferring (PAC1) and VIP-shared (VPAC2) receptor. We have applied gene targeting approach to create mouse lacking PAC1 (PAC1–/–). These mice were viable normoglycemic, but exhibited slight feeding hyperinsulinemia. In vitro, in isolated...
The circadian clock located in the suprachiasmatic nucleus (SCN) organizes autonomic and behavioral rhythms into a near 24 hr time that is adjusted daily to solar cycle via direct projection from retina, retinohypothalamic tract (RHT). This neuronal pathway costores neurotransmitters PACAP glutamate, which seem be important for light-induced resetting of clock. At molecular level genes mPer1 mPer2 are believed target light signaling In this study, we investigated possible role PACAP-type 1...
Sonic hedgehog (SHH) and fibroblast growth factor 2 (FGF2) can both induce neocortical precursors to express the transcription OLIG2 generate oligodendrocyte progenitors (OLPs) in culture. The activity of FGF2 is unaffected by cyclopamine, which blocks Hedgehog signalling, demonstrating that FGF pathway OLP production independent. Unexpectedly, SHH-mediated induction blocked PD173074, a selective inhibitor receptor (FGFR) tyrosine kinase. SHH also depends on mitogen-activated protein kinase...
Few adult neural stem cells have been characterized in vertebrates. Although teleosts continually generate new neurons many regions of the brain after embryogenesis, only two types (NSCs) reported zebrafish: glial forebrain resembling mammalian NSCs, and neuroepithelial cerebellum. Here, following our previous studies on dividing progenitors (Nguyen et al. [1999]: J Comp Neurol 413:385-404.), we further evidenced NSCs optic tectum (OT) juvenile medaka, Oryzias latipes. To detect very slowly...
Investigating neural stem cell (NSC) behaviour in vivo, which is a major area of research, requires NSC models to be developed. We carried out multilevel characterisation the zebrafish embryo peripheral midbrain layer (PML) and identified unique vertebrate progenitor population. Located dorsally transparent midbrain, these large slow-amplifying progenitors (SAPs) are accessible for long-term vivo imaging. They form neuroepithelial adjacent optic tectum, has transitory fast-amplifying (FAPs)...
Abstract Background A current critical need remains in the identification of prognostic and predictive markers early breast cancer. It appears that a distinctive trait cancer cells is their addiction to hyperactivation ribosome biogenesis. Thus, biogenesis might be an innovative source biomarkers evaluated. Methods Here, fibrillarin ( FBL ) was used as surrogate marker due its essential role steps association with poor prognosis when overexpressed. Using 3,275 non-metastatic primary tumors,...
We report here that PAC1 KO females display decreased fertility, whereas male fertility was normal. ICC on pituitary section showed FSH, LH, and prolactin synthesis were not affected in mice. Moreover, the pituitary-gonadal axis responded properly to an acute fasting test Hence, phenotype of null mice provides clear evidence for role receptor reproduction process.
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a potentiator of glucose-induced insulin secretion. PACAP binds to PACAP-specific receptor (PAC1) and VPAC receptors (VPAC1 VPAC2), which share high affinity for vasoactive intestinal (VIP). In the present study, molecular expression isoforms signaling pathways involved in secretory effect were investigated isolated rat mouse pancreatic islets. mRNA encoding PAC1-short, -hop, -very short variants, as well VPAC1 VPAC2, expressed...
Abstract The pituitary adenylate cyclase‐activating polypeptide type‐1 receptor (PAC1) has been involved in the survival and differentiation of neuroblasts during development. This study examined effects various neurotrophins on activity mouse PAC1 promoter/luciferase reporter constructs rat PC12 cells 8‐day‐old cerebellar granule cells. In cells, both differentiating factors such as nerve growth factor (NGF) mitogens epidermal (EGF) insulin factor‐1 (IGF‐1) up‐regulated promoter by 2–4‐fold...
Abstract The role of pituitary adenylate cyclase‐activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC1 receptor‐deficient male mice ( −/− ). effects PACAP on [Ca 2+ ] i were freshly dissociated and the somatodendritic release vasopressin oxytocin, examined intact nuclei. In from wild‐type +/+ ), 100 n m induced an increase whereas heterozygous +/− ) null‐mutant much less effective. had no effect these two...
Abstract The highly conserved POU genes encode homeodomain transcription factors involved in various developmental events, with some, the Brn genes, playing key roles neurogenesis. We investigated evolutionary relationships between these by studying gene complement of a model teleost, medaka ( Oryzias latipes ). identified 17 and carried out comprehensive situ hybridization analysis focusing on optic tectum, cortical structure mesencephalon, which cell positions their differentiation states...
We report that: (1) An increase in the transcription activity is a mechanism by which trophic peptides may regulate expression of PAC1. (2) activation PAC1 promoter does not necessarily correlate with neurotrophin-promoted neuritogenesis. (3) Activation probably an early event since EGF response rather weak and transient PC12 cells. (4) MAP kinase pathway necessary for NGF effect. The antagonism between PACAP observed on already described regulating chromaffin cell proliferation, remains to...