A5050124961- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- RNA modifications and cancer
- interferon and immune responses
- Fish Ecology and Management Studies
- Photoacoustic and Ultrasonic Imaging
- Fish Biology and Ecology Studies
- Marine and fisheries research
- RNA Research and Splicing
Laboratoire d'Informatique Gaspard-Monge
2018-2022
Mécanismes de Tumorigenèse et Thérapies Ciblées
2022
Transgene (France)
2022
UniLaSalle Amiens (ESIEE-Amiens)
2018-2020
Université Gustave Eiffel
2018-2020
Centre National de la Recherche Scientifique
2018-2020
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020
Université Paris-Saclay
2020
Abstract Tissue clearing methods have boosted the microscopic observations of thick samples such as whole-mount mouse or zebrafish. Even with best tissue methods, specimens are not completely transparent and light attenuation increases depth, reducing signal output signal-to-noise ratio. In addition, since acquisition techniques become faster, automated image analysis is now an issue. this context, mounting at large scale often leads to imperfectly aligned oriented samples, which makes...
SUMMARY In recent years, the zebrafish has become a well-established laboratory model. We describe here ZeBraInspector (ZBI) platform for high-content 3D imaging (HCI) of 5 dpf eleuthero-embryos (EEs). This includes mounting method based on 3D-printed stamps to create grid wells in an agarose cast, facilitating batch acquisitions with fast-confocal laser scanning microscope. reference labeling cleared fish fluorescent lipophilic dye. Based this labeling, ZBI software registers EE images,...