A5046517523- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Aquaculture disease management and microbiota
- interferon and immune responses
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Erythrocyte Function and Pathophysiology
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Congenital heart defects research
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- RNA Research and Splicing
- Polyomavirus and related diseases
- Neonatal Respiratory Health Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Physiological and biochemical adaptations
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Phagocytosis and Immune Regulation
Institut Pasteur
2014-2024
Biologie du Développement et Cellules Souches
2013-2024
Centre National de la Recherche Scientifique
2013-2024
Laboratoire de Biologie du Développement
2019
Centre de Gestion Scientifique
2009-2019
Sorbonne Université
2016
Laboratoire de Recherche Scientifique
2010-2013
Centre de Référence Déficits Immunitaires Héréditaires
1999
Stabilité génétique et oncogenèse
1987
Abstract In the zebrafish embryo, only known site of hemopoieisis is an intra-embryonic blood island at junction between trunk and tail that gives rise to erythroid cells. Using video-enhanced differential interference contrast microscopy, as well in-situ hybridization for expression two new hemopoietic marker genes, draculin leucocyte-specific plastin, we show macrophages appear in embryo least early cells, but originate from ventro-lateral mesoderm situated other end just anterior cardiac...
Because the availability of fish genomic data, number reported sequences for type II helical cytokines is rapidly growing, featuring different IFNs including virus-induced (IFNphi) and IFN-gamma, IL-10 with its related (IL-20, IL-22, IL-26). Many candidate receptors exist these various authors have postulated which receptor chain would be involved in functional fish. To date, only zebrafish IFNphi1 has been identified functionally. Three genes encoding IFNphis zebrafish. In addition to...
Abstract The recent description of virus-induced fish IFNs has raised questions about the evolution this complex antiviral system. Identification receptor zebrafish IFN (zIFN) was sought to help resolve these questions. We set up an experimental system study zIFN in course a viral infection embryos. In setting, induced by infection, and we identified zIFN-dependent transcripts. Embryos quickly died from but overexpression increased their survival. took advantage perform vivo loss gain...
Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe novel CHIKV infection model in zebrafish, where viral spread was live-imaged the whole body up to cellular resolution. Infected cells emerged various organs one principal wave with median appearance time of ∼14 hours post infection. Timing infected cell death organ dependent, leading shift localization towards brain. As mammals, triggered strong...
Autophagy, an ancient and highly conserved intracellular degradation process, is viewed as a critical component of innate immunity because its ability to deliver cytosolic bacteria the lysosome. However, role bacterial autophagy in vivo remains poorly understood. The zebrafish (Danio rerio) has emerged vertebrate model for study infections it optically accessible at larval stages when immune system already functional. Here, we have characterized susceptibility larvae Shigella flexneri,...
The 150-base-pairs region located upstream of the transcriptional start site rat albumin gene contains all critical sequences necessary for this gene's tissue-specific expression in hepatoma cells. In transient assays using an improved CAT system or direct mRNA analysis we were able to detect a faithful transcription from promoter albumin-negative dedifferentiated H5 cells which was 250-fold weaker than differentiated H4II producing albumin. This strong tissue specificity could be completely...
Abstract Background In mammals, the members of tripartite motif (TRIM) protein family are involved in various cellular processes including innate immunity against viral infection. Viruses exert strong selective pressures on defense system. Accordingly, antiviral TRIMs have diversified highly through gene expansion, positive selection and alternative splicing. Characterizing immune other vertebrates may enlighten their complex evolution. Results We describe here a large new subfamily...
The beneficial contribution of commensal bacteria to host health and homeostasis led the concept that exogenous non-pathogenic called probiotics could be used limit disease caused by pathogens. However, despite recent progress using gnotobiotic mammal invertebrate models, mechanisms underlying protection afforded probiotic against pathogens remain poorly understood. Here we developed a zebrafish model controlled co-infection in which germ-free raised on axenic living protozoa enabled study...
The early control of potentially invading microbes by our immune system primarily depends on its main professional phagocytes – macrophages and neutrophils. Although the different functions these two cell types have been extensively studied, little is known about their respective contributions to initial microorganisms before onset adaptive responses. naturally translucent zebrafish larva has recently emerged as a powerful model vertebrate in which visualise dynamic interactions between...
Abstract Direct visualization and characterization of Cxcr2 signaling as a mediator systemic neutrophil reaction to local infection. Neutrophils are the first line defense against tissue damage rapidly mobilized sites bacterial However, signals that regulate recruitment not well defined. Here, using photolabel-enabled fate mapping in zebrafish larvae, we show localized otic infection with Pseudomonas aeruginosa induces activation mobilization neutrophils from CHT through signaling. We have...
The zebrafish genome contains a large number of genes encoding potential cytokine receptor as judged by homology to mammalian receptors. sequences are too divergent allow unambiguous assignments all receptors specific cytokines, and only few have been assigned functions functional studies. Among for class II helical cytokines-i.e., IFNs that include virus-induced Ifns (Ifn-) type (Ifn-γ), together with Il-10 its related cytokines (Il-20, Il-22, Il-26)-only the Ifn--specific complexes...
ABSTRACT The zebrafish, Danio rerio , has become a popular vertebrate model for the study of infections, mainly because its excellent optical accessibility at embryonic and larval stages, when innate immune system is already effective. We have thus tested susceptibility zebrafish larvae to human pathogen Listeria monocytogenes gram-positive, facultative, intracellular bacterium that known survive multiply in professional phagocytes causes fatal meningitis abortions. Intravenous injection...