Florence Guivel‐Benhassine
A5042248729- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Bacillus and Francisella bacterial research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Autophagy in Disease and Therapy
- Immunotherapy and Immune Responses
- Complement system in diseases
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Viral Infections and Outbreaks Research
- Immune responses and vaccinations
- Respiratory viral infections research
- Poxvirus research and outbreaks
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- COVID-19 diagnosis using AI
- Bacteriophages and microbial interactions
Institut Pasteur
2015-2025
Université Paris Cité
2021-2025
Institut de Recherche Vaccinale
2020-2025
Centre National de la Recherche Scientifique
2007-2024
Université Paris-Saclay
2023
Immunité et Cancer
2022-2023
Department of Virology
2007-2022
Génomes, biologie cellulaire et thérapeutiques
2010
Centre National pour la Recherche Scientifique et Technique (CNRST)
2009
Fondation de l'Avenir
2008
Chikungunya virus (CHIKV) is a re-emerging arbovirus responsible for massive outbreak currently afflicting the Indian Ocean region and India. Infection from CHIKV typically induces mild disease in humans, characterized by fever, myalgia, arthralgia, rash. Cases of severe infection involving central nervous system (CNS) have recently been described neonates as well adults with underlying conditions. The pathophysiology basis severity are unknown. To address these critical issues, we developed...
An unprecedented epidemic of chikungunya virus (CHIKV) infection recently started in countries the Indian Ocean area, causing an acute and painful syndrome with strong fever, asthenia, skin rash, polyarthritis, lethal cases encephalitis. The basis for disease tropism CHIKV remain unknown. Here, we describe replication characteristics recent clinical strains. Human epithelial endothelial cells, primary fibroblasts and, to a lesser extent, monocyte-derived macrophages, were susceptible allowed...
Dengue viruses (DVs) are responsible for the most medically relevant arboviral diseases. However, molecular interactions mediating DV entry poorly understood. We determined that TIM and TAM proteins, two receptor families mediate phosphatidylserine (PtdSer)-dependent phagocytic removal of apoptotic cells, serve as factors. Cells susceptible to robustly infected after ectopic expression or receptors. Conversely, infection cells is inhibited by anti-TIM anti-TAM antibodies knockdown...
Severe cases of COVID-19 are associated with extensive lung damage and the presence infected multinucleated syncytial pneumocytes. The viral cellular mechanisms regulating formation these syncytia not well understood. Here, we show that SARS-CoV-2-infected cells express Spike protein (S) at their surface fuse ACE2-positive neighboring cells. Expression S without any other proteins triggers formation. Interferon-induced transmembrane (IFITMs), a family restriction factors block entry many...
Chikungunya virus (CHIKV) is the causative agent of an outbreak that began in La Réunion 2005 and remains a major public health concern India, Southeast Asia, southern Europe. CHIKV transmitted to humans by mosquitoes associated disease characterized fever, myalgia, arthralgia, rash. As viral load infected patients declines before appearance neutralizing antibodies, we studied role type I interferon (IFN) pathogenesis. Based on human studies mouse experimentation, show does not directly...
Abstract The SARS-CoV-2 B.1.617 lineage emerged in October 2020 India 1–6 . It has since then become dominant some indian regions and further spread to many countries. includes three main subtypes (B1.617.1, B.1617.2 B.1.617.3), which harbour diverse Spike mutations the N-terminal domain (NTD) receptor binding (RBD) may increase their immune evasion potential. B.1.617.2 is believed faster than other versions. Here, we isolated infectious from a traveller returning India. We examined its...
Different serological assays measuring anti–SARS-CoV-2 antibodies and their neutralizing activity in samples from individuals with severe mild COVID-19 are compared.
Abstract The Fc region of HIV-1 Env-specific broadly neutralizing antibodies (bNAbs) is required for suppressing viraemia, through mechanisms which remain poorly understood. Here, we identify bNAbs that exert antibody-dependent cellular cytotoxicity (ADCC) in cell culture and kill HIV-1-infected lymphocytes natural killer (NK) engagement. These target the CD4-binding site, glycans/V3 V1/V2 loops on gp120, or gp41 moiety. landscape Env epitope exposure at surface sensitivity infected cells to...
Abstract Convergent evolution of SARS-CoV-2 Omicron BA.2, BA.4, and BA.5 lineages has led to the emergence several new subvariants, including BA.2.75.2, BA.4.6. BQ.1.1. The subvariant BQ.1.1 became predominant in many countries December 2022. subvariants carry an additional often redundant set mutations spike, likely responsible for increased transmissibility immune evasion. Here, we established a viral amplification procedure easily isolate strains. We examined their sensitivity 6...
Abstract The unceasing circulation of SARS-CoV-2 leads to the continuous emergence novel viral sublineages. Here, we isolate and characterize XBB.1, XBB.1.5, XBB.1.9.1, XBB.1.16.1, EG.5.1.1, EG.5.1.3, XBF, BA.2.86.1 JN.1 variants, representing >80% circulating variants in January 2024. XBB subvariants carry few but recurrent mutations spike, whereas harbor >30 additional changes. These replicate IGROV-1 no longer Vero E6 are not markedly fusogenic. They potently infect nasal epithelial...
Autophagy is an important survival pathway and can participate in the host response to infection. Studying Chikungunya virus (CHIKV), causative agent of a major epidemic India, Southeast Asia, southern Europe, we reveal novel mechanism by which autophagy limits cell death mortality after We use biochemical studies single multispectral assays demonstrate that direct infection triggers both apoptosis autophagy. CHIKV-induced mediated independent induction endoplasmic reticulum oxidative stress...
Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe novel CHIKV infection model in zebrafish, where viral spread was live-imaged the whole body up to cellular resolution. Infected cells emerged various organs one principal wave with median appearance time of ∼14 hours post infection. Timing infected cell death organ dependent, leading shift localization towards brain. As mammals, triggered strong...
The IFN-inducible antiviral protein tetherin (or BST-2/CD317/HM1.24) impairs release of mature HIV-1 particles from infected cells. Vpu antagonizes the effect tetherin. fate virions trapped at cell surface remains poorly understood. Here, we asked whether HIV cell-to-cell transmission, a major means viral spread. Tetherin-positive or -negative cells, with wild-type ΔVpu HIV, were used as donor cells and cocultivated target lymphocytes. We show that inhibits productive transmission to targets...
Abstract SARS-CoV-2 B.1.1.7 and B.1.351 variants emerged respectively in United Kingdom South Africa spread many countries. Here, we isolated infectious strains examined their sensitivity to anti-SARS-CoV-2 antibodies present sera nasal swabs, comparison with a D614G reference virus. We established novel rapid neutralization assay, based on reporter cells that become GFP+ after overnight infection. was neutralized by 79/83 from convalescent patients collected up 9 months post symptoms,...
Abstract It is of paramount importance to evaluate the prevalence both asymptomatic and symptomatic cases SARS-CoV-2 infection their antibody response profile. Here, we performed a pilot study assess levels anti-SARS-CoV-2 antibodies in samples taken from 491 pre-epidemic individuals, 51 patients Hôpital Bichat (Paris), 209 pauci-symptomatic individuals French Oise region 200 contemporary blood donors. Two in-house ELISA assays, that recognize full-length nucleoprotein (N) or trimeric Spike...
Memory B-cell and antibody responses to the SARS-CoV-2 spike protein contribute long-term immune protection against severe COVID-19, which can also be prevented by antibody-based interventions. Here, wide immunoprofiling in Wuhan COVID-19 convalescents combining serological, cellular, monoclonal explorations revealed humoral immunity coordination. Detailed characterization of a hundred memory antibodies uncovered diversity their repertoire antiviral functions. The latter were influenced...