A5084495244- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Virus-based gene therapy research
- Crystallography and molecular interactions
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- Brucella: diagnosis, epidemiology, treatment
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- Virology and Viral Diseases
- Bacterial Genetics and Biotechnology
- Animal Virus Infections Studies
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- HIV/AIDS drug development and treatment
- Viral Infections and Vectors
- RNA regulation and disease
- Parvovirus B19 Infection Studies
- Complement system in diseases
- RNA and protein synthesis mechanisms
Institut Pasteur
2018-2025
Université Paris Cité
2021-2025
Centre National de la Recherche Scientifique
2021-2024
National Influenza Center
2023
Department of Virology
2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2021
Fundación Instituto Leloir
2012-2021
Memory B-cell and antibody responses to the SARS-CoV-2 spike protein contribute long-term immune protection against severe COVID-19, which can also be prevented by antibody-based interventions. Here, wide immunoprofiling in Wuhan COVID-19 convalescents combining serological, cellular, monoclonal explorations revealed humoral immunity coordination. Detailed characterization of a hundred memory antibodies uncovered diversity their repertoire antiviral functions. The latter were influenced...
Coronaviruses of the subgenus Embecovirus include several relevant pathogens such as human seasonal coronaviruses HKU1 and OC43, bovine coronavirus, porcine hemagglutinating encephalomyelitis virus (PHEV), among others. While sialic acid is thought to be required for embecovirus entry, protein receptors are unknown in most cases. Here we show that PHEV does not require entry uses dipeptidase 1 (DPEP1) a receptor. Cryo-electron microscopy revealed PHEV, unlike other embecoviruses, samples...
Inborn and acquired deficits of type I interferon (IFN) immunity predispose to life-threatening COVID-19 pneumonia. We longitudinally profiled the B cell response mRNA vaccination in SARS-CoV-2 naive patients with inherited TLR7, IRF7, or IFNAR1 deficiency, as well young autoantibodies neutralizing IFNs due autoimmune polyendocrine syndrome type-1 (APS-1) older individuals age-associated IFNs. The receptor-binding domain spike protein (RBD)–specific memory all was quantitatively...
Summary Brucella spp. are facultative intracellular bacteria pathogenic for many mammalian species including humans, causing a disease called brucellosis. Learning how adapts to its niche is crucial understanding pathogenesis mechanism, allowing the development of new and more effective vaccines treatments against resides mostly in ability adapt harsh environmental conditions encountered during host infection such as oxygen depletion. The mechanism by which senses tension triggers adaptation...
Decrypting the B cell ontogeny of HIV-1 broadly neutralizing antibodies (bNAbs) is paramount for vaccine design. Here, we characterized IgA and IgG bNAbs three distinct lineages in a viremic controller, two which comprised only IgG+ or IgA+ blood memory cells; third combined both clonal variants. 7-269 bNAb IgA-only lineage displayed highest capacity despite limited somatic mutation, delayed viral rebound humanized mice. all targeted N332 glycan supersite. The 2.8-Å resolution cryo-EM...
Birnaviruses are a group of double-stranded RNA (dsRNA) viruses infecting birds, fish and insects. Early endosomes (EE) constitute the platform for viral replication. Here, we study mechanism birnaviral targeting EE membranes. Using Infectious Bursal Disease Virus (IBDV) as model, validate that protein 3 (VP3) binds to phosphatidylinositol-3-phosphate (PI3P) present in We identify domain VP3 involved PI3P-binding, named P2 localized core VP3, establish critical role arginine at position 200...
Birnaviruses are a group of double-stranded RNA (dsRNA) viruses infecting birds, fish, and insects. Early endosomes (EE) constitute the platform for viral replication. Here, we study mechanism birnaviral targeting EE membranes. Using Infectious Bursal Disease Virus (IBDV) as model, validate that protein 3 (VP3) binds to phosphatidylinositol-3-phosphate (PI3P) present in We identify domain VP3 involved PI3P-binding, named P2 localized core VP3, establish critical role arginine at position 200...
Summary B rucella is the causative agent of zoonotic disease brucellosis, which endemic in many parts world. The success as pathogen relies its ability to adapt harsh environmental conditions found mammalian hosts. One main adaptations induction expression different genes involved respiration at low oxygen tension. In this report we describe a regulatory network adaptation. We show that abortus PrrBA functional two‐component signal transduction system responds redox status and acts global...
Abstract Recognition and fusion between gametes during fertilization is an ancient process. Protein HAP2, recognized as the primordial eukaryotic gamete fusogen, a structural homolog of viral class II proteins. The mechanisms that regulate HAP2 function, whether virus-fusion-like conformational changes are involved, however, have not been investigated. We report here plus minus green alga Chlamydomonas indeed requires obligate rearrangement on from labile, prefusion form into stable...
After two doses of mRNA vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), patients on dialysis show a defective humoral response, but third dose could increase anti-SARS-CoV-2 spike IgG titers. Responses be different in virus-naive and SARS-CoV-2-recovered dialysis. However, characterization memory B cell response after three is lacking.
Abstract Memory B-cell and antibody responses to the SARS-CoV-2 spike protein contribute long-term immune protection against severe COVID-19, which can also be prevented by antibody-based interventions. Here, wide immunoprofiling in COVID-19 convalescents combining serological, cellular monoclonal explorations, revealed humoral immunity coordination. Detailed characterization of a hundred memory antibodies uncovered diversity their repertoire antiviral functions. The latter were influenced...
Abstract The surface envelope glycoprotein (Env) of all retroviruses mediates virus binding to cells and fusion the viral cellular membranes. A structure-function relationship for HIV Env that belongs Orthoretrovirus subfamily has been well established. Structural information is however largely missing Foamy viruses (FVs), second retroviral subfamily. In this work we present X-ray structure receptor domain (RBD) a simian FV at 2.57 Å resolution, revealing two subdomains an unprecedented...
ABSTRACT The human seasonal coronavirus HKU1-CoV, which causes common colds worldwide, relies on the sequential binding to a cell-surface glycan and TMPRSS2 for entry into target cells. is cell surface protease synthesized as zymogen that undergoes autolytic activation process its substrates. Several respiratory viruses - in particular coronaviruses use proteolytic priming of their spike protein drive membrane fusion upon receptor binding. We describe crystal structure HKU1-CoV domain...