A5063453019- Eicosanoids and Hypertension Pharmacology
- Fatty Acid Research and Health
- Antioxidant Activity and Oxidative Stress
- Inflammatory mediators and NSAID effects
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Free Radicals and Antioxidants
- Alcohol Consumption and Health Effects
- Adipokines, Inflammation, and Metabolic Diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Lipid metabolism and biosynthesis
- Nitric Oxide and Endothelin Effects
- Tribology and Wear Analysis
- Orthopaedic implants and arthroplasty
- Analytical Chemistry and Chromatography
- Advanced Glycation End Products research
- Lubricants and Their Additives
- Vitamin C and Antioxidants Research
- Muscle metabolism and nutrition
- Pharmacology and Obesity Treatment
- Infant Nutrition and Health
- Synthesis and Reactions of Organic Compounds
- Tuberculosis Research and Epidemiology
Université Claude Bernard Lyon 1
2013-2022
Institut National des Sciences Appliquées de Lyon
2013-2022
Centre National de la Recherche Scientifique
1988-2022
Laboratoire CarMeN
2012-2021
Inserm
2012-2021
Hospices Civils de Lyon
2007-2021
Osaka University
2013
Bipar
2013
Institut National de Recherche en Santé Publique
2011
Biologie du Fruit et Pathologie
2011
We have previously reported that endothelial cells synthesize a cytosol-associated, lipoxygenase-derived metabolite, LOX, which acts as chemorepellant and, in so doing, maintains the vessel wall thromboresistance.In this study we demonstrate LOX is 13hydroxylinoleic acid (13-OH-18:2) derived from linoleic and identical to 13-hydroxy-S-cis,l l-transoctadecadienoic acid, measured by both reverse phase high pressure liquid chromatography gas chromatographyJmass spectrometry.In addition,...
Dietary intake of long-chain n-3 PUFA is now widely advised for public health and in medical practice. However, are highly prone to oxidation, producing potentially deleterious 4-hydroxy-2-alkenals. Even so, the impact consuming oxidized on metabolic oxidative stress inflammation poorly described. We therefore studied such effects hypothesized involvement intestinal absorption 4-hydroxy-2-hexenal (4-HHE), an end-product. In vivo, four groups mice were fed 8 weeks high-fat diets containing...
Neuroinflammatory processes are considered a double-edged sword, having both protective and detrimental effects in the brain. Microglia, brain's resident innate immune cells, key component of neuroinflammatory response. There is growing interest developing drugs to target microglia control processes. In this regard, docosahexaenoic acid (DHA), n-3 polyunsaturated fatty acid, promising molecule regulate pro-inflammatory cytokine production. Several works reported that bioavailability DHA...
Previous studies show that polyunsaturated fatty acids (PUFAs) increase the insulin secretory capacity of pancreatic β-cells. We aimed at identifying PUFA-derived mediators and their cellular targets are involved in amplification release from β-cells preexposed to high glucose levels.The content phospholipids INS-1E was determined by lipidomics analysis. High-performance liquid chromatography used identify peroxidation products β-cell cultures. Static dynamic glucose-stimulated secretion...
Our study aimed to establish the complete structure of main dihydroxy conjugated triene issued from lipoxygenation (soybean enzyme) docosahexaenoic acid, named PDX, an isomer protectin/neuroprotectin D1 (PD1/NPD1) described by Bazan and Serhan. NMR approaches other chemical characterization (e.g. GC-MS, HPLC LC-MS/MS) indicated that PDX is 10(S),17(S)-dihydroxy-docosahexa-4Z,7Z,11E,13Z,15E,19Z-enoic acid. The use (18)O(2) mass spectrometry showed a double product. Its differs PD1, with E,Z,E...
A growing body of evidence suggests that exposure to traffic-related air pollution is a risk factor for type 2 diabetes. Ozone, major photochemical pollutant in urban areas, negatively associated with fasting glucose and insulin levels, but most aspects this association remain be elucidated. Using an environmentally realistic concentration (0.8 parts per million), we demonstrated rats ozone induced whole-body resistance oxidative stress, endoplasmic reticulum (ER) c-Jun N-terminal kinase...
Dietary intake of n-3 polyunsaturated fatty acids (PUFA) is primarily recognized to protect against cardiovascular diseases, cognitive dysfunctions and the onset obesity associated metabolic disorders. However, some their properties such as bioavailability can depend on chemical carriers. The objective our study was test hypothesis that nature PUFA carrier results in different effects related adiposity, oxidative stress inflammation.4 groups C57BL/6 mice were fed for 8 weeks low fat (LF)...
Lipid oxidation is implicated in a wide range of pathophysiogical disorders, and leads to reactive compounds such as fatty aldehydes, which the most well known 4-hydroxy-2E-nonenal (4-HNE) issued from 15-hydroperoxyeicosatetraenoic acid (15-HpETE), an arachidonic (AA) product. In addition 15-HpETE, 12(S)-HpETE synthesized by 12-lipoxygenation platelet AA. We first show that 12-HpETE can be degraded vitro into 4-hydroxydodeca-(2E,6Z)-dienal (4-HDDE), specific aldehyde homologous 4-HNE....
Information about lipid oxidation in fresh and stored human milk compared with infant formulas is scarce. We aimed to assess n-6 n-3 PUFA these milks by measuring the 4-hydroxynonenal (4-HNE) 4-hydroxyhexenal (4-HHE) content. Human samples (n = 4), obtained from volunteer mothers, were analyzed after 1 wk at 4 degrees C or 24 h 18 C. Vitamin E malondialdehyde (MDA) measured HPLC fatty acid profile GC. The 4-HHE 4-HNE contents GC-MS. Infant 10) tested; their fat droplet size was laser light...
Abstract Neutrophils play a major role in inflammation by releasing large amounts of reactive oxygen species (ROS) produced NADPH oxidase (NOX) and myeloperoxidase (MPO). This ROS overproduction is mediated phosphorylation the NOX subunits an uncontrolled manner. Therefore, targeting neutrophil would represent promising strategy to moderate activity, lower ROS, other inflammatory agents, such as cytokines leukotrienes, neutrophils. For this purpose, we investigated effects protectin DX...
Increasing evidence suggests that dysregulation of lipid metabolism is associated with neurodegeneration in retinal diseases such as age-related macular degeneration and brain disorders Alzheimer’s Parkinson’s diseases. Lipid storage organelles (lipid droplets, LDs), accumulate many cell types response to stress, it now clear LDs function not only stores but also dynamic regulators the stress response. However, whether these are always protective or can be deleterious unknown. Here, we...
Human platelets were labeled with tracer doses of [14C]arachidonic acid, then fractionated into mixed membranes which are separated intracellular and two different domains surface by high voltage free flow electrophoresis. Each subfraction was analyzed for its phospholipid content. Glycerophospholipids performance liquid chromatography their fatty acids glass capillary gas chromatography. Intracellular appeared substantially depleted in sphingomyelin, while enrichment this seen membranes. PC...
Our results show that oxidative stress induces COX‐2 expression through the production of 4‐HNE which activates p38MAPKinase, suggesting links and chronic inflammation activation cyclooxygenase.
Hydroxy-alkenals, such as 4-hydroxy-2(E)-nonenal (4-HNE; from n-6 fatty acids), are degradation products of acid hydroperoxides, including those generated by free radical attack membrane polyunsaturated acyl moieties. The cytotoxic effects hydroxy-alkenals well known and mainly attributable to their interaction with different molecules form covalent adducts. Indeed, ethanolamine phospholipids (PEs) can be covalently modified in a cellular system hydroxy-alkenals, 4-HNE,...
OBJECTIVE Vascular endothelial cells (VECs) downregulate their rate of glucose uptake in response to hyperglycemia by decreasing the expression typical transporter GLUT-1. Hitherto, we discovered critical roles for protein calreticulin and arachidonic acid–metabolizing enzyme 12-lipoxygenase this autoregulatory process. The hypothesis that 4-hydroxydodeca-(2E,6Z)-dienal (4-HDDE), peroxidation product 12-lipoxygenase, mediates downregulatory mechanism activating peroxisome...
Docosahexaenoic acid (DHA), an important component of marine lipids, exhibits antiinflammatory activity related to some its oxygenated metabolites, such as neuroprotectin/protectin D1 [NPD1/PD1;10(R),17(S)-dihydroxy-docosa-4Z,7Z, 11E,13E,15Z,19Zhexaenoic acid] produced through the 15-lipoxygenase pathway. However, other metabolites from DHA can be this pathway, and polyunsaturated fatty acids (PUFAs) nutritional value may well. Their biological activities remain unknown. Isomers protectin...