A5054589726- Brucella: diagnosis, epidemiology, treatment
- Escherichia coli research studies
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- Atherosclerosis and Cardiovascular Diseases
- Burkholderia infections and melioidosis
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Prion Diseases and Protein Misfolding
- Developmental Biology and Gene Regulation
- Neurogenetic and Muscular Disorders Research
- Neurogenesis and neuroplasticity mechanisms
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Research and Splicing
- T-cell and B-cell Immunology
- Animal Diversity and Health Studies
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Venomous Animal Envenomation and Studies
- Leptospirosis research and findings
- Peripheral Neuropathies and Disorders
- Rabies epidemiology and control
- Cellular transport and secretion
- Bacterial Infections and Vaccines
Centre d’Immunologie de Marseille-Luminy
2012-2025
Inserm
2014-2025
Aix-Marseille Université
2012-2025
Centre National de la Recherche Scientifique
2014-2025
Délégation Provence et Corse
2013
Institut National de Recherche en Santé Publique
2013
Institut de Biologie du Développement Marseille
1997-2011
Universidad de Costa Rica
1990-2008
BackgroundThe brucellae are facultative intracellular bacteria that cause brucellosis, one of the major neglected zoonoses. In endemic areas, vaccination is only effective way to control this disease. Brucella melitensis Rev 1 a vaccine against brucellosis sheep and goat caused by B. melitensis, commonest source human infection. However, carries smooth lipopolysaccharide with an O-polysaccharide elicits antibodies interfering in serodiagnosis, problem eradication campaigns. Because this,...
Innate immunity recognizes bacterial molecules bearing pathogen-associated molecular patterns to launch inflammatory responses leading the activation of adaptive immunity. However, lipopolysaccharide (LPS) gram-negative bacterium Brucella lacks a marked pattern, and it has been postulated that this delays development immunity, creating gap is critical for reach intracellular replicative niche. We found B. abortus mutant in wadC gene displayed disrupted LPS core while keeping both...
Bacteria of the genus Brucella are facultative intracellular parasites that cause brucellosis, a severe animal and human disease. Recently, group taxonomists merged brucellae with primarily free-living, phylogenetically related
ABSTRACT Muscle-derived factors are known to be important for the survival of developing spinal motoneurons, but molecules involved have not been characterized. Hepatocyte growth factor/scatter factor (HGF/SF) plays an role in muscle development and motoneuron axon outgrowth. We show that HGF/SF has potent neurotrophic activity (EC50=2 pM) a subpopulation (40%) purified embryonic rat motoneurons. Moreover, is essential component muscle-derived support since blocking antibodies specifically...
The cytokines ciliary neurotrophic factor (CNTF) and leukemia inhibitory (LIF) signal through a receptor complex formed between two transmembrane proteins, gp130 LIFRbeta. In addition, CNTF also uses ligand-binding component which is anchored to the cell membrane. case of cardiotrophin-1 (CT-1), LIFRbeta required in cardiomyocytes, but this has not been proven neurons, published data suggest that motoneurons may use different complex. We used Lifrbeta knockout mice assess requirement for...
The survival of central neurons depends on multiple neurotrophic factors produced by different cell types. We demonstrate that media conditioned muscle and Schwann lines show strong synergistic effects purified embryonic day 14.5 rat motoneurons in culture. Different evidence implicate glial line-derived factor (GDNF) cardiotrophin-1 (CT-1) this synergy. Their expression the environment motoneuron is compartmentalized: gdnf transcripts are expressed principally lines, whereas ct-1 mRNA...
The intracellular pathogen Brucella abortus survives and replicates inside host cells within an endoplasmic reticulum (ER)-derived replicative organelle named the "Brucella-containing vacuole" (BCV). Here, we developed a subcellular fractionation method to isolate BCVs characterize for first time protein composition of its niche. After identification BCV membrane proteins by 2 dimensional (2D) gel electrophoresis mass spectrometry, focused on two eukaryotic proteins:...
Brucellae replicate in a vacuole derived from the endoplasmic reticulum (ER) epithelial cells, macrophages, and dendritic cells. In animals, trophoblasts are also key cellular targets where brucellae efficiently association with ER. Therefore, we investigated ability of Brucella spp. to infect human using both immortalized primary trophoblasts. extensively proliferated within different subpopulations trophoblasts, suggesting that they constitute an important niche cases fetal-maternal...
Most bacterial infections induce the activation of polymorphonuclear neutrophils (PMNs), enhance their microbicidal function, and promote survival these leukocytes for protracted periods time. Brucella abortus is a stealthy pathogen that evades innate immunity, barely activates PMNs, resists killing mechanisms phagocytes. Intriguing clinical signs observed during brucellosis are low numbers infected PMNs in target organs neutropenia proportion patients; features deserve further attention....
The structures of the lipooligosaccharides from Brucella melitensis mutants affected in WbkD and ManBcore proteins have been fully characterized using NMR spectroscopy. results revealed that disruption wbkD gives rise to a rough lipopolysaccharide (R-LPS) with complete core structure (β-d-Glcp-(1→4)-α-Kdop-(2→4)[β-d-GlcpN-(1→6)-β-d-GlcpN-(1→4)[β-d-GlcpN-(1→6)]-β-d-GlcpN-(1→3)-α-d-Manp-(1→5)]-α-Kdop-(2→6)-β-d-GlcpN3N4P-(1→6)-α-d-GlcpN3N1P), addition components lacking one terminal β-d-GlcpN...
Cervical lymph nodes (CLN) are the first encountered by material taking oral route. To study their role in orally acquired infections, we analyzed 307 patients of up to 14 years treated university clinic Skopje, Macedonia, for brucellosis, a zoonotic bacterial disease frequently ingestion contaminated dairy products. From these children, 36% had lymphadenopathy. Among infected lymphadenopathy with CLN being only affected was significantly more frequent as compared those contact animals (83%...
Polymorphonuclear neutrophils (PMNs) are the first line of defense against microbial pathogens. In addition to their role in innate immunity, PMNs may also regulate events related adaptive immunity. To investigate influence immune response during chronic bacterial infections, we explored course brucellosis antibody PMN-depleted C57BL/6 mice and neutropenic mutant Genista mouse model. We demonstrate that at later times infection, Brucella abortus is killed more efficiently absence than...
Immune evasion strategies of Brucella, the etiologic agent brucellosis, a global zoonosis, remain partially understood. The omentum, tertiary lymphoid organ part visceral adipose tissue, has never been explored as Brucella reservoir. We report that B. abortus infects and replicates within murine omental macrophages. Throughout chronic phase infection, omentum accumulates macrophages, monocytes neutrophils. maintenance PD-L1+Sca-1+ neutrophils in depends on wadC-encoded determinant LPS....
Plasmacytoid dendritic cells (pDCs), professional type I IFN-producing cells, have been implicated in host responses against bacterial infections. However, their role defense is debated, and the operating molecular mechanisms are unknown. Certain signaling lymphocyte activation molecule family (SLAMF) members act as microbial sensors modulate immune functions response to infection. Here, human blood transcriptomic analyses reveal involvement of SLAMF7 SLAMF8 many infectious diseases, with...
The precise control of motor neuron (MN) death and survival following initial innervation skeletal muscle targets is a key step in sculpting functional system, but how this regulated at the level individual pools remains unclear. Hepatocyte growth factor (HGF) its receptor Met play developmental roles both MNs. We generated mice (termed “ Nes-Met ”) which met inactivated from midembryonic stages onward CNS only. Adult animals showed behavioral defects suggestive impaired pectoral muscles....
The brucellae are α-Proteobacteria facultative intracellular parasites that cause an important zoonosis. These bacteria escape early detection by innate immunity, ability associated to the absence of marked pathogen-associated molecular patterns in cell envelope lipopolysaccharide, lipoproteins and flagellin. We show here that, contrast outer membrane ornithine lipids (OL) other Gram negative bacteria, Brucella abortus OL lack a pattern activity. identified two genes (olsB olsA) generating...
So far, hematopoietic stem cells (HSC) are considered the source of mature immune cells, latter being only ones capable mounting an response. Recent evidence shows HSC can also directly sense cytokines released upon infection/inflammation and pathogen-associated molecular pattern interaction while keeping a long-term memory previously encountered signals. Direct sensing danger signals by induces early myeloid commitment, increases effector cell numbers, contributes to efficient Here, using...