A5029227537- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Burkholderia infections and melioidosis
- Animal Nutrition and Physiology
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Herpesvirus Infections and Treatments
- Listeria monocytogenes in Food Safety
- Bacterial biofilms and quorum sensing
- Animal Diversity and Health Studies
- Probiotics and Fermented Foods
- HIV Research and Treatment
- Virus-based gene therapy research
- Microbial infections and disease research
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- Bacterial Infections and Vaccines
- Antimicrobial Peptides and Activities
- Gut microbiota and health
Consejo Superior de Investigaciones Científicas
2005-2024
Gobierno de Navarra
2012-2024
Agrobiotechnology Institute
2013-2024
Universidad Pablo de Olavide
2023-2024
Universidad de Sevilla
2023-2024
Département Santé Animale
2024
Universidad Publica de Navarra
2009-2018
Universidad de Navarra
1995-2009
Gobierno de Aragón
2005-2007
Centro de Investigación y Tecnología Agroalimentaria de Aragón
1998-2006
Abstract Engineered live bacteria could provide a new modality for treating lung infections, major cause of mortality worldwide. In the present study, we engineered genome-reduced human bacterium, Mycoplasma pneumoniae , to treat ventilator-associated pneumonia, disease with high hospital when associated Pseudomonas aeruginosa biofilms. After validating biosafety an attenuated M. chassis in mice, introduced four transgenes into chromosome by transposition implement bactericidal and biofilm...
Two mutants showing increased sensitivity to polycations and surfactants were obtained by transposon mutagenesis of virulent Brucella abortus 2308 Nal r . These showed no obvious in vitro growth defects produced smooth‐type lipopolysaccharides. However, they hardly multiplied or persisted mouse spleens, displayed reduced invasiveness macrophages HeLa cells, lost the ability inhibit lysosome fusion unable replicate intracellularly. Subsequent DNA analyses identified a two‐component regulatory...
BackgroundThe brucellae are facultative intracellular bacteria that cause brucellosis, one of the major neglected zoonoses. In endemic areas, vaccination is only effective way to control this disease. Brucella melitensis Rev 1 a vaccine against brucellosis sheep and goat caused by B. melitensis, commonest source human infection. However, carries smooth lipopolysaccharide with an O-polysaccharide elicits antibodies interfering in serodiagnosis, problem eradication campaigns. Because this,...
Innate immunity recognizes bacterial molecules bearing pathogen-associated molecular patterns to launch inflammatory responses leading the activation of adaptive immunity. However, lipopolysaccharide (LPS) gram-negative bacterium Brucella lacks a marked pattern, and it has been postulated that this delays development immunity, creating gap is critical for reach intracellular replicative niche. We found B. abortus mutant in wadC gene displayed disrupted LPS core while keeping both...
ABSTRACT Bacteriological diagnosis of brucellosis is performed by culturing animal samples directly on both Farrell medium (FM) and modified Thayer-Martin (mTM). However, despite inhibiting most contaminating microorganisms, FM also inhibits the growth Brucella ovis some B. melitensis abortus strains. In contrast, mTM adequate for all species but only partially inhibitory contaminants. Moreover, performance culture media isolating suis has never been established properly. We first determined...
A study was performed to evaluate the previously described PCR (C. Romero, C. Gamazo, M. Pardo, and I. López-Goñi, J. Clin. Microbiol. 33:615-617, 1995) for diagnosis of brucellosis in dairy cattle. Milk samples from 56 Brucella milk culture-positive cattle 37 Brucella-free herds were examined DNA by specific antibodies an indirect enzyme-linked immunosorbent assay (ELISA). The specificities both tests 100% when testing 49 infected positive (87.5% sensitivity), 55 ELISA (98.2% sensitivity)....
Brucella abortus rough lipopolysaccharide (LPS) mutants were obtained by transposon insertion into two wbk genes (wbkA [putative glycosyltransferase; formerly rfbU] and per [perosamine synthetase]), manB (pmm [phosphomannomutase; rfbK]), an unassigned gene. Consistent with gene-predicted roles, electrophoretic analysis, 2-keto-3-manno-D-octulosonate measurements, immunoblots monoclonal antibodies to O-polysaccharide, outer inner core epitopes showed no O-polysaccharide expression LPS defects...
Phosphatidylcholine (PC) is a typical eukaryotic phospholipid absent from most prokaryotes. Thus, its presence in some intracellular bacteria intriguing as it may constitute host mimicry. The role of PC Brucella abortus was examined by generating mutants pcs (BApcs) and pmtA (BApmtA), which encode key enzymes the two bacterial biosynthetic routes, choline methyl-transferase pathways. In rich medium, BApcs double mutant BApcspmtA but not BApmtA displayed reduced growth, increased...
Phage lytic proteins are promising antimicrobials that could complement conventional antibiotics and help to combat multi-drug resistant bacteria cause important human animal infections. Here, we report the characterization of endolysin LysRODI (encoded by staphylophage phiIPLA-RODI) its application as a prophylactic mastitis treatment. The main properties were compared with those LysA72 phiIPLA35) chimeric protein CHAPSH3b (derived from virion-associated peptidoglycan hydrolase HydH5...
ABSTRACT The genes coding for the five outer membrane proteins (OMPs) of Omp25/Omp31 family expected to be located in (OM) rough virulent Brucella ovis PA were inactivated evaluate their role virulence and OM properties. properties mutant strains mutants complemented with corresponding wild-type analyzed, comparison parental strain B. abortus RB51, several tests: (i) binding anti-Omp25 anti-Omp31 monoclonal antibodies, (ii) autoagglutination bacterial suspensions, (iii) assessment...
ABSTRACT Nontypeable Haemophilus influenzae (NTHi) is a frequent commensal of the human nasopharynx that causes opportunistic infection in immunocompromised individuals. Existing evidence associates lipooligosaccharide (LOS) with disease, but specific and relative contributions NTHi LOS modifications to virulence properties bacterium have not been comprehensively addressed. Using strain 375, an isolate for which detailed structure has determined, we compared systematically set isogenic...
The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria facultative intracellular parasites and thus able to adjust their metabolism extra- environments encountered during an infectious cycle. However, this aspect Brucella biology is imperfectly understood, nutrients available in niche unknown. Here, we investigated central pathways C used by abortus deleting putative fructose-1,6-bisphosphatase (fbp glpX), phosphoenolpyruvate carboxykinase...
Summary The epidemiology of subclinical salmonellosis in wild birds a region high Salmonella prevalence pigs was studied. Three hundred and seventy‐nine faecal samples from 921 trapped 31 locations nearby pig premises, 431 581 10 natural settings far farms were analysed for the presence spp. Positive serotyped antimicrobial resistance (AR). Phage typing pulsed‐field gel electrophoresis (PFGE) on Typhimurium isolates also carried out. overall proportion ‐positive 1.85% (95% CI = 0.93, 2.77)....
ABSTRACT The ISO 6579:2002/Amd 1:2007 (ISO) standard has been the bacteriological method used in European Union for detection of Salmonella spp. pig mesenteric lymph nodes (MLN), but there are no published estimates diagnostic sensitivity (Se) this matrix. Here, Se (Se ) was estimated on 675 samples selected from two populations with different prevalences (14 farms a ≥20% prevalence and 13 <20% prevalence) through use latent-class models concert Bayesian inference, assuming 100%...
Brucella spp. are Gram-negative bacteria that behave as facultative intracellular parasites of a variety mammals. This genus includes smooth (S) and rough (R) species carry S R lipopolysaccharides (LPS), respectively. S-LPS is virulence factor, mutants affected in the O-polysaccharide (R mutants), core oligosaccharide or both show attenuation. However, B. ovis naturally virulent sheep. We studied role LPS by mutating orthologues wadA, wadB wadC, three genes known to encode...
A longitudinal study was conducted to investigate the presence of multidrug antimicrobial resistance (multi-AR) in Salmonella enterica pigs reared under conventional preventative medicine programmes Spain and possible association multi-AR with ceftiofur or tulathromycin treatment during pre-weaning period. Groups 7-day-old piglets were treated by intramuscular injection on four farms (n = 40 per farm) another farm). control group untreated 30 present each farm. Faecal swabs collected for S....
ABSTRACT The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption bvrS or bvrR generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, complement. Consequently, role in virulence was examined. Similar mutants, omp3a omp3b displayed increased attachment cells, indicating surface alterations. However, they showed unaltered permeability;...
Forty‐one pregnant sheep showing positive immune responses to Brucella melitensis in serological or allergic tests were selected from naturally infected flocks and kept an isolated pen for lambing. The resulting 62 lambs maintained the same with their dams during lactation. When weaned, slaughtered bacteriological study reared a clean pen. Fourteen ewes excreted B lactation 17 found be postmortem. was not seven (three born dams) which died after birth eight seronegative (four between two...