Abstract Engineered live bacteria could provide a new modality for treating lung infections, major cause of mortality worldwide. In the present study, we engineered genome-reduced human bacterium, Mycoplasma pneumoniae , to treat ventilator-associated pneumonia, disease with high hospital when associated Pseudomonas aeruginosa biofilms. After validating biosafety an attenuated M. chassis in mice, introduced four transgenes into chromosome by transposition implement bactericidal and biofilm...

Lactococci have been associated with fetal and neonatal infections in humans cattle. Here we describe a case of reproductive loss attributed to Lactococcus petauri lemur. A full-term black-and-white ruffed lemur (Varecia variegata) was found dead the indoor area zoologic exhibit. Classification as late-term abortion or stillbirth unclear precise gestational time unknown. medical checkup dam revealed fever neutrophilic leukocytosis; recovery followed treatment enrofloxacin. The main...

African swine fever (ASF) is a notifiable disease that in recent years has spread remarkably Europe and Asia. Eurasian wild boar (Sus scrofa) plays key role the maintenance of pathogen. Here we examined gross pathology infection with highly virulent, hemadsorbing genotype II ASF virus (ASFV) strain. To this end, six boars were intramuscularly inoculated 10 HAD50 Arm07 ASFV strain, 11 allowed to come into direct contact animals. No animals survived infection. Clinical course, pathological...

Human–wildlife conflicts may have devastating consequences for fauna due to targeting by humans of wildlife populations suspected predated livestock. Suspicion depredation extensively raised livestock vultures in Europe has triggered public administration-led forensic investigations intended distinguish between predation and scavenging order compensate farmers attacks on their In this study, gross histological analyses were carried out cases domestic animal griffon (Gyps fulvus) over a...

Neurobrucellosis is a shared condition of cetaceans and humans. However, the pathogenesis immune response in cetacean neurobrucellosis has not been extensively studied. In this multicentric investigation, 21 striped dolphin ( Stenella coeruleoalba) Brucella ceti) cases diagnosed over 10-year period (2012-2022) were retrospectively evaluated. For each case, morphological changes assessed by evaluating histological parameters. Furthermore, immunohistochemical expression antigen, glial...

Virginia opossums ( Didelphis virginiana) are the only native marsupials in United States of America and Canada. Females have a reproductive tract consisting complex duplex (bifurcated) system, with 2 ovaries, uteri uterine tubes, vaginal composed lateral vaginae, sinuses, urogenital sinus. Few conditions been described these marsupials. A 2-y-old, female opossum was presented because hematuria found dead shortly after admission. The autopsy revealed left torsion pyovagina. Streptococcus...

Clostridium colinum causes ulcerative enteritis in several avian species. The disease is particularly prevalent quail, and it therefore colloquially known as quail disease. pathogenesis of the infection poorly understood. A retrospective study C. submitted for necropsy diagnostic work up to California Animal Health Food Safety Laboratory System between 1992 2022 was performed. reports were reviewed, polymerase chain reaction (PCR) ( 16S rRNA ) performed on formalin-fixed, paraffin-embedded...

A 7-y-old male neutered crossbreed dog was presented to a veterinary referral hospital with 9-mo history of progressive non-ambulatory tetraparesis. magnetic resonance imaging (MRI) study revealed multiple T2-weighted hyperintense intradural nodular lesions within the cervical spinal cord and nerves that were contrast-enhancing in T1-weighted post-contrast images. Neurologic signs progressed despite steroid treatment second MRI slight enlargement previously seen lesions. The euthanized due...

The olive oil sector is currently adapting its traditional function to also become a supplier of high-value by-products that possess antioxidant, anti-inflammatory and antimicrobial properties. In this study, we evaluated the effect fermented defatted alperujo (FDA) on intestinal health laying hens. morphology duodenal cecal mucosa, composition microbiota productivity batch hens were after FDA supplementation. At early life stages, significant differences (p < 0.001) observed in villi height...

Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, wildlife. Ticks have been suggested as vectors, long‐term carriers, amplifiers of Brucella . In this study, ticks from wildlife ungulate hosts living in hunting reserves central region Spain were collected during 6‐year period, pooled, screened for spp. by PCR. Aiming to correlate DNA presence with infections hosts, liver samples deceased ungulates coming the showing positive result tested using...

Feline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) has been described as an inflammatory disorder with component etiopathogenesis that is still unknown. Sixteen intestinal samples from two veterinary diagnostic services (2014–2017) were included in the study. A histopathological criterion classified cases into three grades (mild, moderate, and severe) according to distribution of lesions course. An immunohistochemical study collagen I, III, fibronectin, transforming growth...

Porcine brucellosis, caused by Brucella suis (B. suis), is a notifiable disease causing significant economic losses in production systems. Most infected pigs may act as carriers and shed B. even if asymptomatic. This can contribute to environmental persistence, thus hindering control efforts. Here, the environment offspring were investigated during after outbreak at sow breeding farm. The diagnosis of sows (n = 1,140) was performed culture polymerase chain reaction (PCR) from vaginal swabs,...

Aeromonas salmonicida is one of the major threats to world aquaculture, causing fish furunculosis and high mortality rates in cultured fish, particularly salmonids. Although spp. a thoroughly studied pathogen, little known regarding aeromoniasis sturgeons. After outbreak, four juvenile sturgeons (Acipenser baerii) were submitted for autopsy tissue samples collected histopathological microbiological studies. The external examination revealed size heterogenicity, skin hyperpigmentation reduced...

The expanding presence of red foxes ( Vulpes vulpes) in urban and suburban regions could potentially lead to increased instances human aggression towards this species. We studied 10 deceased that were submitted by law enforcement agencies the metropolitan area Madrid 2014–2022 because suspected abuse. Forensic autopsies performed establish cause manner death. In 4 cases, death was deemed unnatural, involving blunt-force trauma n = 2), asphyxia resulting from hanging 1), firearm injury 1)....

Porcine brucellosis, which is caused by Brucella suis biovar (bv) 2, a re-emerging disease that causes reproductive problems in pigs Europe. The pathogenesis and lesions of B. intrauterine infection are poorly characterized; characterization could facilitate the diagnosis investigation porcine brucellosis. We collected samples placentas fetuses for histologic microbiologic studies during an outbreak abortions on pig-breeding farm Spain. was cultured from vaginal swabs obtained sows had...

Nutraceuticals are not only nutritionally beneficial for animals but also their use as feed supplements may reduce environmental contamination. The effect of fermented defatted "alperujo," an olive oil by-product, supplementation on the intestinal health broiler chickens was assessed by analyzing mucosal morphology duodenum and cecum. microbiota cecum characterized V3-V4 region 16S rRNA gene days 7, 14, 21, 28, 35, 42. Supplemented broilers from 14 to 35 D age showed increase in villus...

Flavobacterium psychrophilum affects many cultured fish species and is considered one of the most important bacterial pathogens causing substantial economic losses in salmonid aquaculture worldwide. Here, F. was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) nested PCR as aetiological agent mortality diseased juvenile Siberian sturgeons (Acipenser baerii) reared on a freshwater farm. Diseased were lethargic displayed dark skin...

Tauopathies are a group of neurodegenerative diseases characterized by the pathological aggregation hyperphosphorylated tau in neurons and glia. Primary tauopathies not uncommon humans but exceptional other species. We evaluate clinical, neuropathological, genetic alterations related to pathology 16 cats aged from 1 21 years with different clinical backgrounds. Interestingly, 10-year-old female cat presented six-year progressive history mental status gait abnormalities. The imaging study...

Salmonella spp. contaminates egg and poultry meat leading to foodborne infections in humans. The emergence of antimicrobial-resistant strains has limited the use antimicrobials. We aimed determine effects food supplement, fermented defatted ‘alperujo’ (FDA), a modified olive oil by-product, on Typhimurium colonisation broilers. One hundred twenty 1-day-old broilers were divided into four experimental groups—two control groups two treated groups, challenged with S. at day 7 or 21. On days 7,...

Salmonella spp. contaminates egg and poultry meat leading to foodborne infections in humans. The emergence of antimicrobial-resistant strains limit the use antimicrobials. We aimed determine effects food supplement, fermented defatted &amp;lsquo;alperujo&amp;rsquo;, a modified olive-oil by-product, on Typhimurium colonisation broilers. One hundred twenty 1-day-old broilers were divided into four experimental groups: 2 control groups treated groups, challenged with S. at day 7 or 21. On days...

In this study, the effects of co-administration with biscuit flour and fermented defatted “alperujo” (FDA) on gut health were evaluated in a batch laying hens (Hy-Line 2015) commercial farm. Animals divided into two groups: control group treatment group; histological morphometric analyses all sections intestine (duodenum, jejunum, ileum, cecum rectum) performed at 10, 18, 25, 50 75 weeks age. During whole productive period, decrease mortality rate (p = 0.01) was observed treated hens, as...

Lymphoma is the most common malignant hematopoietic neoplasm in domestic felines. Twenty-two cases of feline epitheliotropic duodenal T-cell lymphoma were characterized morphologically and immunohistochemically (CD3, Pax5, Ki-67), Bcl-2 immunoexpression was established. Most shorthair cats (88.2%), with a mean age 11.2 years. All lymphomas CD3+, low-to-moderate expression Ki-67 (<30%). A correlation between tumoral pattern infiltration lamina propria intraepithelial distribution neoplastic...