A5005061862- Dermatology and Skin Diseases
- Acne and Rosacea Treatments and Effects
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Nail Diseases and Treatments
- Pneumonia and Respiratory Infections
- Enzyme Production and Characterization
- Photosynthetic Processes and Mechanisms
- Bacterial biofilms and quorum sensing
- Pharmacological Effects of Natural Compounds
- Complementary and Alternative Medicine Studies
- Redox biology and oxidative stress
- Dermatologic Treatments and Research
- Glycosylation and Glycoproteins Research
- Bacterial Identification and Susceptibility Testing
- Polysaccharides and Plant Cell Walls
- Dermatological diseases and infestations
- Biochemical and Structural Characterization
- Genomics and Phylogenetic Studies
- Advancements in Transdermal Drug Delivery
- Infections and bacterial resistance
- Protein Structure and Dynamics
- Mycobacterium research and diagnosis
- Psoriasis: Treatment and Pathogenesis
Centre for Genomic Regulation
2013-2021
S-Biomedic (Germany)
2018
Barcelona Institute for Science and Technology
2018
Universitat Pompeu Fabra
2010-2013
European Bioinformatics Institute
2013
European Synchrotron Radiation Facility
2009
The University of Tokyo
2008
European Science Foundation
2007
University of Tübingen
2007
The skin is colonized by a large number of microorganisms, most which are beneficial or harmless. However, disease states have specific microbiome compositions that different from those healthy skin. Gut modulation through fecal transplant has been proven as valid therapeutic strategy in diseases such Clostridium difficile infections. Therefore, techniques to modulate the composition may become an interesting option affecting psoriasis acne vulgaris.Here, we used mixtures components alter...
Abstract Aggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as biofilms, are associated with several infectious diseases. One main components these biofilms is alginate, homo- hetero-polysaccharide that consists β-D-mannuronate (M) α-L-guluronate (G) units. Alginate lyases degrade this sugar have been proposed biotherapeutic agents to dissolve P. biofilms. However, there contradictory reports in literature regarding...
Enhanced cyan fluorescent protein (ECFP) and its variant Cerulean are genetically encoded fluorophores widely used as donors in FRET-based cell imaging experiments. First, we have confirmed through denaturation experiments that the double-peak spectroscopic signature of these proteins originates from indole ring chromophore. Then, to explain improvement fluorescence properties compared those ECFP, determined high-resolution crystal structures two at physiological pH performed molecular...
Human skin microbiome dysbiosis can have clinical consequences. Characterizing taxonomic composition of bacterial communities associated with disorders is important for dermatological advancement in both diagnosis and novel treatments. This study aims to analyze improve the accuracy classification bacteria MinION™ nanopore sequencing using a defined mock community sample. We compared Oxford Nanopore Technologies recommended procedures concluded that their protocols highly bias relative...
Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains the modulate microbiome and thereby lead a reduction This pilot study evaluates safety efficacy modulation on acne-prone skin. The had 2 phases: active induction (5% benzoyl peroxide gel, 7 days) interventional C. treatment (5 weeks). Patients were randomized either topical formulations PT1 (2 Single Locus Sequence Typing [SLST] type C3 K8, 50%...
Synchrotrons are now producing thousands of macromolecular structures each year. The need for complementary techniques available on site has progressively emerged, either to assess the relevance structure a protein or monitor changes that may occur during X-ray diffraction data collection. Microspectrophotometers in UV–visible absorbance fluorescence mode have evolved over past few decades become instruments choice perform such tests. Described here recent improvements microspectrophotometer...
Systems metabolomics, the identification and quantification of cellular metabolites their integration with genomics proteomics data, promises valuable functional insights into biology. However, technical constraints, sample complexity issues lack suitable complementary quantitative data sets prevented accomplishing such studies in past. Here, we present an integrative metabolomics study genome-reduced bacterium Mycoplasma pneumoniae. We experimentally analysed its metabolome using a...
Reovirus attachment protein sigma1 mediates engagement of receptors on the surface target cells and undergoes dramatic conformational rearrangements during viral disassembly in endocytic pathway. The is a filamentous, trimeric molecule with globular beta-barrel head domain. An unusual cluster aspartic acid residues sandwiched between hydrophobic tyrosines located at subunit interface. A 1.75-A structure domain now reveals two water molecules interface that are held strictly position interact...
Modern cloning methods are independent from restriction enzyme recognition sites. However, nearly all current still require the introduction of overlaps by PCR, which can introduce undesired mutations. Here, we investigated whether needed for DNA assembly be synthesized in situ and tested if de novo synthesis sequences simultaneously combined with larger double-stranded fragments. We showed a set 44 experiments that 20 bp single-stranded oligonucleotides. Short 30-255 oligonucleotides...
Cutibacterium acnes is a predominant bacterium on human skin and generally regarded as commensal. Recently, the abundantly secreted protein produced by C. , RoxP, was shown to alleviate radical-induced cell damage, presumably via antioxidant activity, which could potentially be harnessed fortify barrier function. The aim of this study determine structure RoxP elucidate mechanisms behind its antioxidative effect. Here, we present solution revealing compact immunoglobulin-like domain...
Bacteria of the genus Mycoplasma have recently attracted considerable interest as model organisms in synthetic and systems biology. In particular, pneumoniae is one most intensively studied field However, genetic manipulation these bacteria often difficult due to lack efficient some intrinsic peculiarities such an aberrant code. One major disadvantage working with M. replicating plasmids that can be used for complementation mutants expression proteins. this study, we analysed genomic region...
Cutibacterium acnes (C. acnes) is a gram-positive bacterium and member of the human skin microbiome. Despite being most abundant commensal, certain members have been associated with common inflammatory disorders such as acne vulgaris. The availability complete genome sequences from various C. clades enabled identification putative methyltransferases, some them potentially belonging to restriction-modification (R-M) systems which protect host invading DNA. However, little known on whether...
Ferredoxin-NAD(P) + reductase (FNR) is a key enzyme that catalyzes the photoreduction of NAD(P) to generate NAD(P)H during final step photosynthetic electron-transport chain.FNR from green sulfur bacterium Chlorobium tepidum homodimeric with molecular weight 90 kDa; it shares high level amino-acid sequence identity thioredoxin rather than conventional plant-type FNRs.In order understand structural basis ferredoxin-dependency this unique FNR, C. FNR has been heterologously expressed, purified...
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Background The skin is colonized by a large number of microorganisms, which most are beneficial or harmless. However, disease states have specific microbiome compositions that different from those healthy skin. Gut modulation through fecal transplant has proven as valid therapeutic strategy in diseases such Clostridium difficile infections. Therefore, techniques to modulate the composition may become an interesting option affecting psoriasis acnes vulgaris. Here we used mixtures components...