A5070386713- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Galectins and Cancer Biology
- Atherosclerosis and Cardiovascular Diseases
- Burkholderia infections and melioidosis
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Zoonotic diseases and public health
- Animal Disease Management and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Infections and bacterial resistance
- Animal Diversity and Health Studies
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Microbial infections and disease research
- Pharmaceutical and Antibiotic Environmental Impacts
- Identification and Quantification in Food
- Antibiotic Resistance in Bacteria
- Nosocomial Infections in ICU
- Gastric Cancer Management and Outcomes
- Rabies epidemiology and control
- Plant Pathogenic Bacteria Studies
- Livestock and Poultry Management
Universidad Nacional
2016-2025
Universidad de Costa Rica
2008-2020
Wellcome Sanger Institute
2017
University of Liverpool
2017
Costa Rican Department of Social Security
2017
Universidad Hispanoamerica
2014
Mote Marine Laboratory
2009
Gobierno de Aragón
2008
To unravel the strategy by which Brucella abortus establishes chronic infections, we explored its early interaction with innate immunity.Brucella did not induce proinflammatory responses as demonstrated absence of leukocyte recruitment, humoral or cellular blood changes in mice. hampered neutrophil (PMN) function and PMN depletion influence course infection. barely induced cytokines consumed complement, was strongly resistant to bactericidal peptides, extracts serum. LPS (BrLPS),...
Innate immunity recognizes bacterial molecules bearing pathogen-associated molecular patterns to launch inflammatory responses leading the activation of adaptive immunity. However, lipopolysaccharide (LPS) gram-negative bacterium Brucella lacks a marked pattern, and it has been postulated that this delays development immunity, creating gap is critical for reach intracellular replicative niche. We found B. abortus mutant in wadC gene displayed disrupted LPS core while keeping both...
Bacteria of the genus Brucella are facultative intracellular parasites that cause brucellosis, a severe animal and human disease. Recently, group taxonomists merged brucellae with primarily free-living, phylogenetically related
Abstract Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. ceti was isolated from cerebrospinal fluid of 6 dolphins 1 fetus. S. coeruleoalba constitutes a highly susceptible host potential reservoir for B. transmission.
Abstract Several species of Brucella are known to be zoonotic, but B. neotomae infection has been thought limited wood rats. In 2008 and 2011, however, was isolated from cerebrospinal fluid 2 men with neurobrucellosis. The nonzoonotic status should reassessed.
Background During evolution, innate immunity has been tuned to recognize pathogen-associated molecular patterns. However, some α-Proteobacteria are stealthy intracellular pathogens not readily detected by this system. Brucella members follow strategy and highly virulent, but other Brucellaceae like Ochrobactrum rhizosphere inhabitants only opportunistic pathogens. To gain insight into the emergence of strategy, we compared these two phylogenetically close biologically divergent bacteria....
Most bacterial infections induce the activation of polymorphonuclear neutrophils (PMNs), enhance their microbicidal function, and promote survival these leukocytes for protracted periods time. Brucella abortus is a stealthy pathogen that evades innate immunity, barely activates PMNs, resists killing mechanisms phagocytes. Intriguing clinical signs observed during brucellosis are low numbers infected PMNs in target organs neutropenia proportion patients; features deserve further attention....
Polymorphonuclear neutrophils (PMNs) are the first line of defense against microbial pathogens. In addition to their role in innate immunity, PMNs may also regulate events related adaptive immunity. To investigate influence immune response during chronic bacterial infections, we explored course brucellosis antibody PMN-depleted C57BL/6 mice and neutropenic mutant Genista mouse model. We demonstrate that at later times infection, Brucella abortus is killed more efficiently absence than...
Canine brucellosis caused by Brucella canis is a disease of dogs and zoonotic risk. B. harbors most the virulence determinants defined for genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium an intracellular pathogen. Studies outbreaks in kennel facilities indicated infected displaying clinical signs did present hematological alterations. A virulent strain isolated from those readily replicated different organs mice...
The global poultry trend toward the more responsible use of antibiotics is becoming recurrent and has demanded need to generate new natural alternatives. Probiotics have gained importance as an option growth promoters. This study aimed evaluate Bacillus subtillis QST713 a substitute for antibiotic promoter (BMD). A total 150 male broilers were assigned three dietary treatments: 1) control diet (CO), 2) + 500 g/t BMD (AGP), 3) 100 B. subtilis (PB), respectively. Each treatment was monitored 5...
Desmodus rotundus, vampire bats, transmit dangerous infections, and brucellosis is a hazardous zoonotic disease, two adversities that coexist in the subtropical tropical areas of American continent. Here, we report 47.89% Brucella infection prevalence colony bats inhabiting rainforest Costa Rica. The bacterium induced placentitis fetal death bats. Wide-range phenotypic genotypic characterization placed organisms as new pathogenic species named nosferati sp. nov., isolated from bat tissues,...
ABSTRACT The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption bvrS or bvrR generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, complement. Consequently, role in virulence was examined. Similar mutants, omp3a omp3b displayed increased attachment cells, indicating surface alterations. However, they showed unaltered permeability;...
Intracellular bacterial pathogens probably arose when their ancestor adapted from a free-living environment to an intracellular one, leading clonal bacteria with smaller genomes and less sources of genetic plasticity. Still, this plasticity is needed respond the challenges posed by host. Members Brucella genus are facultative-extracellular responsible for causing brucellosis in variety mammals. The various species keep different host preferences, virulence, zoonotic potential despite having...
Brucella abortus is a stealthy intracellular bacterial pathogen of animals and humans. This bacterium promotes the premature cell death neutrophils (PMN) resists killing action these leukocytes. B. abortus-infected PMNs presented phosphatidylserine (PS) as "eat me" signal on surface. promoted direct contacts between macrophages (Mϕs) favored phagocytosis infected dying PMNs. Once inside Mϕs, replicated within Mϕs at significantly higher numbers than when were with bacteria alone. The high...
Brucellosis is a bacterial infectious disease affecting wide range of mammals and neglected zoonosis caused by species the genetically homogenous genus Brucella. As in most studies on diseases, research brucellosis carried out using reference strains as canonical models to understand mechanisms underlying host pathogen interactions. We performed whole genome sequencing (WGS) analysis strain Brucella abortus 2308 routinely used our laboratory, including manual curated annotation accessible an...
Immune evasion strategies of Brucella, the etiologic agent brucellosis, a global zoonosis, remain partially understood. The omentum, tertiary lymphoid organ part visceral adipose tissue, has never been explored as Brucella reservoir. We report that B. abortus infects and replicates within murine omental macrophages. Throughout chronic phase infection, omentum accumulates macrophages, monocytes neutrophils. maintenance PD-L1+Sca-1+ neutrophils in depends on wadC-encoded determinant LPS....
The brucellae are α-Proteobacteria facultative intracellular parasites that cause an important zoonosis. These bacteria escape early detection by innate immunity, ability associated to the absence of marked pathogen-associated molecular patterns in cell envelope lipopolysaccharide, lipoproteins and flagellin. We show here that, contrast outer membrane ornithine lipids (OL) other Gram negative bacteria, Brucella abortus OL lack a pattern activity. identified two genes (olsB olsA) generating...
Brucellosis, caused by Brucella abortus is a major disease of cattle and zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), total 765 herds (13078 bovines) from six regions CR were randomly sampled during 2012–2013. A non-random sample 7907 (532199 regions, arriving for diagnoses 2014–2016 Rican Animal Health Service was also studied. The estimated Rose Bengal test (RBT) ranged 10.5%-11.4%; alternatively, testing RBT positives iELISA, 4.1%-6.0%,...