A5017667142- Brucella: diagnosis, epidemiology, treatment
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Burkholderia infections and melioidosis
- Distributed and Parallel Computing Systems
- Vibrio bacteria research studies
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Scientific Computing and Data Management
- Galectins and Cancer Biology
- Advanced Data Storage Technologies
- Cystic Fibrosis Research Advances
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Peer-to-Peer Network Technologies
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Animal Diversity and Health Studies
- Collagen: Extraction and Characterization
- Prion Diseases and Protein Misfolding
- Laser Applications in Dentistry and Medicine
- Sleep and Work-Related Fatigue
Université de Montpellier
2014-2024
Inserm
2013-2024
Bacterial Virulence and Chronic Infections
2016-2024
Université de Nîmes
2013-2023
Xperi (Ireland)
2023
Centre Hospitalier Universitaire de Nîmes
2009-2023
Guiana Space Centre
2023
Centre Hospitalier Andrée Rosemon
2023
University of French Guiana
2023
Imperial College Healthcare NHS Trust
2016-2022
Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep Malta fever humans. The genome of B. strain 16M was sequenced found to contain 3,294,935 bp distributed over two circular chromosomes 2,117,144 1,177,787 encoding 3,197 ORFs. By using the bioinformatics suite ERGO, 2,487 (78%) ORFs were assigned functions. origins replication are similar those other α-proteobacteria. Housekeeping genes, including involved DNA replication,...
Analysis of a Tn blaM mutant Brucella suis 1330, identified as being unable to multiply in Hela cells, allowed us identify 11 860 bp region the B. genome encoding type IV secretion system, homologous VirB system Agrobacterium tumefaciens and Ptl Bordetella pertussis . DNA sequence revealed 12 open reading frames (ORFs) homologues proteins present pTi plasmid with similar genetic organization, twelfth ORF putative lipoprotein, protein involved mating pair formation during bacterial...
Stable transposon-generated auxotrophic mutations in aroA, purA, and purE or aroA purA together were introduced into Salmonella typhimurium strains which virulent mice. Strains harboring any of these attenuated when tested BALB/c less than strains. Both mutants persisted for several weeks the livers spleens mice after intravenous infection, although numbers viable cells detected at various times infection differed. a higher level effective live vaccines given intravenously orally....
A type IV secretion system similar to the VirB of phytopathogen Agrobacterium tumefaciens is essential for intracellular survival and multiplication mammalian pathogen Brucella . Reverse transcriptase–PCR showed that 12 genes encoding suis form an operon. Semiquantitative measurements virB mRNA levels by slot blotting transcription operon, but not flanking genes, regulated environmental factors in vitro Flow cytometry used measure green fluorescent protein expression from promoter confirmed...
Brucella species include important zoonotic pathogens that have a substantial impact on both agriculture and human health throughout the world. Brucellae are thought of as "stealth pathogens" escape recognition by host innate immune response, modulate acquired evade intracellular destruction. We analyzed genome sequences members family Brucellaceae to assess its evolutionary history from likely free-living soil-based progenitors into highly successful pathogens. Phylogenetic analysis split...
Brucellosis is a neglected zoonosis. It causes acute febrile illness and potentially debilitating chronic infection in humans, livestock has substantial socioeconomic impact. Over the past two decades, improvements have been made to better understand various aspects of human animal brucellosis. Meanwhile, especially developing world, immense challenges that remain controlling eradicating brucellosis are novel diagnostics tools efficacious vaccines. Here, we will focus on remarkable issues...
Bacteria of the genus Brucella are facultative intracellular parasites that cause brucellosis, a severe animal and human disease. Recently, group taxonomists merged brucellae with primarily free-living, phylogenetically related
ABSTRACT Bacteria of the genus Brucella are facultative intracellular pathogens which have developed capacity to survive and multiply in professional nonprofessional phagocytes. The genetic basis this aspect virulence is still poorly understood. To identify new factors, we adapted signature-tagged transposon mutagenesis, has been used essentially animal models, an vitro human macrophage infection model. A library 1,152 suis 1330 tagged mini-Tn 5 Km2 mutants, 12 pools, was screened for...
Tumor necrosis factor α-converting enzyme (TACE) is responsible for the shedding of cell surface TNF. Studies suggest that reactive oxygen species (ROS) mediate up-regulation TACE activity by direct oxidization or modification protein. However, these investigations have been largely based upon nonphysiological stimulation promonocytic lines which may respond and process differently from primary cells. Furthermore, investigators relied substrate as a surrogate quantification. We addressed...
Bacteria belonging to the "Burkholderia cepacia complex" (Bcc) often cause fatal pulmonary infections in cystic fibrosis patients, yet little is know about underlying molecular mechanisms. These Gram-negative bacteria can adopt an intracellular lifestyle, although their ability replicate intracellularly has been difficult demonstrate. Here we show that Bcc survive and multiply macrophages of zebrafish embryos. Local dissemination by nonlytic release from infected cells was followed...
Recently, the worldwide propagation of clonal CTX-M-15-producing Escherichia coli isolates, namely ST131 and O25b:H4, has been reported. Like majority extra-intestinal pathogenic E. pandemic clone belongs to phylogenetic group B2, recently shown be highly virulent in a mouse model, even though it lacks several genes encoding key virulence factors (Pap, Cnf1 HlyA). Using two animal models, Caenorhabditis elegans zebrafish embryos, we assessed three strains (2 CTX-M-15- producing urine 1...
Brucella are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. The genus has recently expanded from 6 to 11 species, all of which were associated with mammals; natural host range amphibians after some reports atypical strains frogs. Here we describe the first in depth phenotypic and genetic characterization isolated frog. Strain B13-0095 was Pac-Man frog (Ceratophyrus ornate) at veterinary hospital Texas initially misidentified as Ochrobactrum...
We have studied the genomic structure and constructed Spe I, Pac I I‐ Ceu restriction maps of four biovars pathogenic bacterium Brucella suis . B biovar 1 has two chromosomes 2.1 Mb 1.15 Mb, similar to those other species: melitensis , abortus ovis neotomae Two were also observed in genome 2 4, but with sizes 1.85 1.35 whereas only one chromosome a size 3.1 was found 3. show that differences number can be explained by rearrangements at chromosomal regions containing three rrn genes. The...
PacI and SpeI restriction maps were obtained for the two chromosomes of each six species genus Brucella: B. melitensis, abortus, suis, canis, ovis, neotomae. Three complementary techniques used: hybridization with replicons as probes, cross-hybridization fragments, a new mapping method. For type strain, unique I-SceI site was introduced in replicons, location sites determined by linearization at site, partial digestion, end labeling fragments. The genetic highly conserved. However, numerous...
Bacteria of the Burkholderia cepacia complex (Bcc) can cause devastating pulmonary infections in cystic fibrosis (CF) patients, yet precise mechanisms underlying inflammation, recurrent exacerbations and transition from chronic stages to acute infection septicemia are not known. Bcc bacteria generally believed have a predominant extracellular biofilm life style infected CF lungs, similar Pseudomonas aeruginosa, but this has been challenged by clinical observations which show predominantly...