A5013053965- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Visual perception and processing mechanisms
- HIV Research and Treatment
- RNA modifications and cancer
- Spatial Neglect and Hemispheric Dysfunction
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Spatial Cognition and Navigation
- School Health and Nursing Education
- Selenium in Biological Systems
- Trace Elements in Health
- Motor Control and Adaptation
- Tactile and Sensory Interactions
- RNA Interference and Gene Delivery
- Hemispheric Asymmetry in Neuroscience
- Musculoskeletal pain and rehabilitation
- Virus-based gene therapy research
- Balance, Gait, and Falls Prevention
- interferon and immune responses
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Immune Cell Function and Interaction
- Ophthalmology and Visual Impairment Studies
Centre National de la Recherche Scientifique
2016-2025
Centre International de Recherche en Infectiologie
2016-2025
Université Claude Bernard Lyon 1
2016-2025
Inserm
2016-2025
École Normale Supérieure de Lyon
2016-2025
Laboratoire de Psychologie et NeuroCognition
1998-2019
Université Grenoble Alpes
2011-2019
Université de Strasbourg
2018
Aix-Marseille Université
2018
Ministère du travail, de l'emploi et de l'insertion
2018
Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells living organisms. However, their delivery into target can be technically challenging when working with primary or vivo. Here, we use engineered murine leukemia virus-like particles loaded Cas9-sgRNA ribonucleoproteins (Nanoblades) to induce efficient genome-editing cell lines including human induced pluripotent stem cells, hematopoietic mouse bone-marrow cells. Transgene-free Nanoblades are also...
Significance Translational control is a cornerstone of gene-expression regulation in physiological and pathological contexts. The contribution nonribosomal factors, including messenger RNAs (mRNAs) mRNA-bound to translational have been extensively studied. Recently, the hypothesis ribosome-mediated emerged, which proposes that cells produce ribosomes different composition displaying properties. This work reveals ribosomal RNA 2′-O-methylation can be modulated human ribosomes, at key...
ABSTRACT The 5′ leader of the human immunodeficiency virus type 1 (HIV-1) genomic RNA contains highly structured domains involved in key steps viral life cycle. These inhibit cap-dependent protein synthesis. Here we report that HIV-1 harbors an internal ribosome entry site (IRES) capable driving synthesis during G 2 /M cell cycle phase which initiation is inhibited. IRES was delineated with bicistronic mRNAs vitro and ex vivo assays. spans nucleotides 104 to 336 partially overlaps major...
Mechanisms of drug-tolerance remain poorly understood and have been linked to genomic but also non-genomic processes. 5-fluorouracil (5-FU), the most widely used chemotherapy in oncology is associated with resistance. While prescribed as an inhibitor DNA replication, 5-FU alters all RNA pathways. Here, we show that treatment leads production fluorinated ribosomes exhibiting altered translational activities. incorporated into ribosomal RNAs mature cancer cell lines, colorectal xenografts,...
Prime Editing can rewrite genes in living cells by allowing point mutations, deletions, or insertion of small DNA sequences with high precision. However, its safe and efficient delivery into human stem remains a technical challenge. In this report, we engineer Nanoscribes, virus-like particles that encapsidate ribonucleoprotein complexes the system allow their recipient cells. We identify key features unlock potential including use multiple fusogens, improvement pegRNAs structures, encoding...
Human immunodeficiency virus type-1 (HIV-1) is a complex retrovirus that relies on alternative splicing, translational, and post-translational mechanisms to produce over 15 functional proteins from its single ~10 kb transcriptional unit. Using ribosome profiling, nascent protein labeling, RNA sequencing, whole-proteomics of infected CD4 + T lymphocytes, we characterized the transcriptional, landscape during infection. While viral infection exerts significant impact host transcript abundance,...
The cap-binding eukaryotic initiation factor, eIF4E, is a key target for the regulation of translation in mammalian cells and widely thought to be present at very low molar concentrations. Here we observations with reticulocyte lysate that challenge this view. When ribosomes are harvested by centrifugation, most (75%) eIF4E remains postribosomal supernatant (PRS). In reconstituted system find ribosome-associated alone can sustain much overall activity, suggesting factor PRS functionally...
Here, we show a novel molecular mechanism promoted by the DEAD-box RNA helicase DDX3 for translation of HIV-1 genomic RNA. This occurs through adenosine triphosphate-dependent formation initiation complex that is assembled at 5′ m7GTP cap mRNA. due to property substitute factor eIF4E in binding structure where it nucleates core DDX3/PABP/eIF4G trimeric on By using fluorescence situ hybridization coupled indirect immunofluorescence, further this viral ribonucleoprotein addressed...
ISG20 is a broad spectrum antiviral protein thought to directly degrade viral RNA. However, this mechanism of inhibition remains controversial. Using the Vesicular Stomatitis Virus (VSV) as model RNA virus, we show here that interferes with replication by decreasing synthesis in absence degradation. Importantly, demonstrate exerts translational control over large panel non-self substrates including those originating from transfected DNA, while sparing endogenous transcripts. This activity...
Selenium is an essential trace element in our diet, crucial for the composition of human selenoproteins, which include 25 genes such as glutathione peroxidases and thioredoxin reductases. The regulation selenoproteome primarily hinges on bioavailability selenium, either from dietary sources or cell culture media. This selenium-dependent control follows a specific hierarchy, with “housekeeping” selenoproteins maintaining constant expression while “stress-regulated” counterparts respond to...
microRNAs (miRNAs) regulate gene expression at multiple levels by repressing translation, stimulating deadenylation and inducing the premature decay of target messenger RNAs (mRNAs). Although mechanism which miRNAs repress translation has been widely studied, precise step targeted molecular insights such repression are still evasive. Here, we have used our newly designed in vitro system, allows to study miRNA effect on independently deadenylation. By using specific inhibitors various stages...