A5003629568- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Gene expression and cancer classification
- Tissue Engineering and Regenerative Medicine
- Bioinformatics and Genomic Networks
- 3D Printing in Biomedical Research
- Pharmacological Effects of Natural Compounds
- RNA Interference and Gene Delivery
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Sarcoma Diagnosis and Treatment
- Skin Protection and Aging
- Virus-based gene therapy research
- Climate Change Communication and Perception
- Viral Infections and Immunology Research
- Single-cell and spatial transcriptomics
- Conferences and Exhibitions Management
- Telomeres, Telomerase, and Senescence
- Transcranial Magnetic Stimulation Studies
- Fibroblast Growth Factor Research
- Genetics, Aging, and Longevity in Model Organisms
- Computational Drug Discovery Methods
- Plant Virus Research Studies
Genopole (France)
2022
Institut des Cellules Souches pour le Traitement et l'Étude des Maladies Monogéniques
2017-2022
Inserm
2017-2022
Université Grenoble Alpes
2022
Centre Hospitalier Universitaire de Grenoble
2022
Centre National de la Recherche Scientifique
2016-2022
Genethon (France)
2018
Université d'Évry Val-d'Essonne
2015-2018
Université de Bordeaux
2016
Immunology from Concept and Experiments to Translation
2011-2016
Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells living organisms. However, their delivery into target can be technically challenging when working with primary or vivo. Here, we use engineered murine leukemia virus-like particles loaded Cas9-sgRNA ribonucleoproteins (Nanoblades) to induce efficient genome-editing cell lines including human induced pluripotent stem cells, hematopoietic mouse bone-marrow cells. Transgene-free Nanoblades are also...
Abstract Background Duchenne muscular dystrophy (DMD) causes severe disability of children and death young men, with an incidence approximately 1/5000 male births. Symptoms appear in early childhood, a diagnosis made mostly around 4 years old, time where the amount muscle damage is already significant, preventing therapeutic interventions that could be more efficient at halting disease progression. In meantime, precise moment which phenotypes arise—even asymptomatically—is still unknown....
Duchenne muscular dystrophy (DMD) affects myofibers and muscle stem cells, causing progressive degeneration repair defects. It was unknown whether dystrophic myoblasts—the effector cells of growth regeneration—are affected. Using transcriptomic, genome-scale metabolic modelling functional analyses, we demonstrate, for the first time, convergent abnormalities in primary mouse human myoblasts. In Dmd mdx myoblasts lacking full-length dystrophin, expression 170 genes significantly altered....
Duchenne muscular dystrophy (DMD) is a devastating X-linked recessive genetic myopathy. DMD physiopathology still not fully understood and prenatal onset suspected but difficult to address. The bone morphogenetic protein 4 (BMP4) critical signaling molecule involved in mesoderm commitment. Human induced pluripotent stem cells (hiPSCs) from healthy individuals human embryonic (hESCs) treated with BMP4 allowed us model the early steps of myogenesis normal contexts. Unexpectedly, 72h following...
Rhabdomyosarcoma (RMS) is the main form of pediatric soft-tissue sarcoma. Its cure rate has not notably improved in last 20 years following relapse, and lack reliable preclinical models hampered design new therapies. This particularly true for highly heterogeneous fusion-negative RMS (FNRMS). Although methods have been proposed to establish FNRMS organoids, their efficiency remains limited date, both terms derivation ability accurately mimic original tumor. Here, we present development a...
A growing portion of scientists realises the need to not only alert about climate change, but also change their professional practices. range tools have emerged promote more sustainable activities, yet many struggle go beyond simple awareness-raising create concrete transition actions. Here we propose a game-based support system MaTerre180’ , which has been designed build scenarios greenhouse gas (GHG) emission reductions in academic community. After providing common scientific background...
Faecalibacterium prausnitzii is abundant in the healthy human intestinal microbiota, and absence or scarcity of this bacterium has been linked with inflammatory diseases metabolic disorders. F . thus shows promise as a next-generation probiotic for use restoring balance gut microbial flora and, due to its strong anti-inflammatory properties, treatment certain pathological conditions. However, very little information available about gene function regulation species. Here, we utilized systems...
Tissue engineering strategies aim at characterizing and optimizing the cellular component that is combined with biomaterials, for improved tissue regeneration. Here, we present immunoMap of apical papilla, native from which SCAPs are derived. We characterized stem cell niches correspond to a minority population cells expressing Mesenchymal stromal/Stem Cell (CD90, CD105, CD146) stemness (SSEA4 CD49f) markers as well endothelial (VWF, CD31). Based on colocalization TKS5 cortactin markers,...
Mutations in the DMD gene lead to Duchenne muscular dystrophy (DMD), a severe neuromuscular disorder affecting young boys as they acquire motor functions. is typically diagnosed at 2-4 years of age, but absence dystrophin has negative impacts on skeletal muscles before overt symptoms appear patients, which poses serious challenge current standards care. Here, we investigated consequences deficiency during muscle development. We used single-cell transcriptome profiling characterize myogenic...
Mouse embryonic stem cells (mESCs) are expanded and maintained pluripotent in vitro the presence of leukemia inhibitory factor (LIF), an IL6 cytokine family member which displays pleiotropic functions, depending on both cell maturity type. LIF withdrawal leads to heterogeneous differentiation mESCs with a proportion differentiated apoptosising. During withdrawal, sequentially enter reversible irreversible phase during addition induces different effects. However regulators effectors...
Background. Human embryonic stem cells (hESCs) are pluripotent derived from the inner cell mass of in vitro fertilised blastocysts, which can either be maintained an undifferentiated state or committed into lineages under determined culture conditions. These offer great potential for regenerative medicine, but at present, little is known about mechanisms that regulate hESC stemness; particular, role cell-cell and cell-extracellular matrix interactions remain relatively unexplored. Methods...
Summary Mutations in the DMD gene lead to Duchenne muscular dystrophy, a severe X-linked neuromuscular disorder that manifests itself as young boys acquire motor functions. is typically diagnosed at 2 4 years of age, but absence dystrophin negatively impacts muscle structure and function before overt symptoms appear patients, which poses serious challenge optimization standards care. In this report, we investigated early consequences deficiency during skeletal development. We used...
ABSTRACT Duchenne muscular dystrophy (DMD) causes severe disability of children and death young men, with an incidence approximately 1/5,000 male births. Symptoms appear in early childhood, a diagnosis made around 4 years old, time where the amount muscle damage is already significant, preventing therapeutic interventions that could be more efficient at halting disease progression. In meantime, precise moment which phenotypes arise – even asymptomatically still unknown. Thus, there critical...
Gene silencing techniques, including RNA interference methodologies, are widely used in reverse genetics to study the role of specific genes biological processes. has become easier implement thanks RNAi Consortium (TRC), which developed libraries short hairpin (shRNA) sequences pseudotyped lentiviral particles capable targeting most human and mouse genomes. However, a problem is lack simple method titrate homemade particle product, making it difficult optimize standardize shRNA experiments....
Abstract Duchenne muscular dystrophy (DMD) affects myofibers and muscle stem cells (SC), causing progressive degeneration repair defects. It was not known whether dystrophic myoblasts—the effector of growth regeneration—are affected. Using a combination transcriptomic, molecular, functional analyses, genome-scale metabolic modelling, we demonstrate, for the first time, convergent cell-autonomous abnormalities in primary mouse human myoblasts. In Dmd mdx myoblasts lacking full-length...
Abstract Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells living organisms. However, their delivery into target can be technically challenging when working with primary or vivo . Using engineered murine leukemia virus-like particles loaded Cas9/sgRNA ribonucleoproteins (“Nanoblades”), we were able to induce efficient genome-editing cell lines including human induced pluripotent stem cells, hematopoietic mouse bone-marrow cells. Transgene-free...
Abstract Faecalibacterium prausnitzii is abundant in the healthy human intestinal microbiota, and absence or scarcity of this bacterium has been linked with inflammatory diseases metabolic disorders. F. thus shows promise as a next-generation probiotic for use restoring balance gut microbial flora and, due to its strong anti-inflammatory properties, treatment certain pathological conditions. However, very little information available about gene function regulation species. Here, we utilized...
Abstract Rhabdomyosarcoma (RMS) is the main form of soft-tissue sarcoma in children and adolescents. For 20 years, despite international clinical trials, its cure rate has not really improved, remains stuck at 20% case relapse. The definition new effective therapeutic combinations hampered by lack reliable models, which complicate transposition promising results obtained pre-clinical studies into efficient solutions for young patients. Inter-patient heterogeneity, particularly so-called...
Background. Human embryonic stem cells (hESCs) are pluripotent derived from the inner cell mass of in vitro fertilised blastocysts, which can either be maintained an undifferentiated state or committed into lineages under determined culture conditions. These offer great potential for regenerative medicine, but at present, little is known about mechanisms that regulate hESC stemness; particular, role cell-cell and cell-extracellular matrix interactions remain relatively unexplored. Methods...
Background. Human embryonic stem cells (hESCs) are pluripotent derived from the inner cell mass of in vitro fertilised blastocysts, which can either be maintained an undifferentiated state or committed into lineages under determined culture conditions. These offer great potential for regenerative medicine, but at present, little is known about mechanisms that regulate hESC stemness; particular, role cell-cell and cell-extracellular matrix interactions remain relatively unexplored. Methods...
Background. Human embryonic stem cells (hESCs) are pluripotent derived from the inner cell mass of in vitro fertilised blastocysts, which can either be maintained an undifferentiated state or committed into lineages under determined culture conditions. These offer great potential for regenerative medicine, but at present, little is known about mechanisms that regulate hESC stemness; particular, role cell-cell and cell-extracellular matrix interactions remain relatively unexplored. Methods...