A5081509779- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- RNA Research and Splicing
- HIV Research and Treatment
- RNA modifications and cancer
- SARS-CoV-2 detection and testing
- Vaccine Coverage and Hesitancy
- RNA regulation and disease
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Biosensors and Analytical Detection
- Virus-based gene therapy research
- COVID-19 and Mental Health
- Influenza Virus Research Studies
- Long-Term Effects of COVID-19
- HIV/AIDS Research and Interventions
- Viral gastroenteritis research and epidemiology
- COVID-19 diagnosis using AI
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- HIV/AIDS drug development and treatment
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
University of Chile
2015-2024
Millennium Institute on Immunology and Immunotherapy
2021-2024
Instituto Pasteur
2021
Weatherford College
2021
Institut Pasteur
2021
Universidad de Magallanes
2021
Institute of Biomedical Science
2021
Jewish General Hospital
2012-2017
McGill University
2012-2015
Pontificia Universidad Católica de Chile
2007-2013
The development of effective vaccines against coronavirus disease 2019 is a global priority. CoronaVac an inactivated severe acute respiratory syndrome 2 (SARS-CoV-2) vaccine with promising safety and immunogenicity profiles. This article reports results obtained for healthy Chilean adults aged ≥18 years in phase 3 clinical trial.Volunteers randomly received doses or placebo, separated by weeks. A total 434 volunteers were enrolled, 397 18-59 37 ≥60 years. Solicited unsolicited adverse...
Convalescent plasma (CP), despite limited evidence on its efficacy, is being widely used as a compassionate therapy for hospitalized patients with COVID-19. We aimed to evaluate the efficacy and safety of early CP in COVID-19 progression.The study was an open-label, single-center randomized clinical trial performed academic medical center Santiago, Chile, from May 10, 2020, July 18, final follow-up until August 17, 2020. The included within first 7 days symptom onset, presenting risk factors...
ABSTRACT Background The newly described SARS-CoV-2 lineage C.37 was recently classified as a variant of interest by the WHO (Lambda variant) based on its high circulation rates in South American countries and presence critical mutations spike protein. impact such infectivity immune escape from neutralizing antibodies are entirely unknown. Methods We performed pseudotyped virus neutralization assay determined Lambda using plasma samples healthcare workers (HCW) two centers Santiago, Chile who...
Screening, testing and contact tracing plays a pivotal role in control of the COVID-19 pandemic. To enable this it is necessary to increase capacity. This study compared SARS-CoV-2 rapid antigen test (RAT) RT-PCR 842 asymptomatic individuals from Tarapacá, Chile. A sensitivity 69.86%, specificity 99.61%, PPV 94.44% NPP 97.22% with Ct values (Ct > 27) that were significantly higher among false-negative RAT reported. These results support fact might have significant impact on identification...
The ongoing COVID-19 pandemic has had a significant impact worldwide, with an incommensurable social and economic burden. rapid development of safe protective vaccines against this disease is global priority. CoronaVac vaccine prototype based on inactivated SARS-CoV-2, which shown promising safety immunogenicity profiles in pre-clinical studies phase 1/2 trials China. To day, four 3 clinical are Brazil, Indonesia, Turkey, Chile. This article reports the results obtained subgroup participants...
CoronaVac is an inactivated SARS-CoV-2 vaccine approved by the World Health Organization (WHO). Previous studies reported increased levels of neutralizing antibodies and specific T cells 2 4 weeks after two doses CoronaVac; these were significantly reduced at 6 to 8 months doses. Here, we report effect a booster dose on anti-SARS-CoV-2 immune response generated against variants concern (VOCs), Delta Omicron, in adults participating phase III clinical trial Chile. Volunteers immunized with...
An HIV-1–based pseudotype allows the rapid quantification of neutralizing antibody titers against SARS-CoV-2 spike protein.
During retroviral replication, the full-length RNA serves both as mRNA and genomic RNA. However, mechanisms by which HIV-1 Gag protein selects two molecules that will be packaged into nascent virions remain poorly understood. Here, we demonstrate deposition of N6-methyladenosine (m6A) regulates packaging. While m6A METTL3/METTL14 onto was associated with increased synthesis reduced packaging, FTO-mediated demethylation promoted incorporation viral particles. Interestingly, associates...
The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. entry of virus into cell is one main targets inhibiting SARS-CoV-2 infection. Natural products are an important source therapeutic alternatives diseases. Pseudotyped viruses allow study viral inhibitors, and due their simplicity, they screening a large number candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 D614G spike glycoprotein test its ability infect...
The 5′ leader of the human immunodeficiency virus type 1 (HIV-1) genomic RNA harbors an internal ribosome entry site (IRES) that is functional during G2/M phase cell cycle. Here we show translation initiation mediated by HIV-1 IRES requires participation trans-acting cellular factors other than canonical translational machinery. We used 'standard' chemical and enzymatic probes 'RNA SHAPE' analysis to model structure show, means a footprinting assay, extracts provide protections regions...
Abstract The detection of viruses in sewage is a method environmental surveillance, which allows evaluating the circulation different community. This study presents first results surveillance to detect SARS-CoV-2 virus Santiago, Chile. Using ultracentrifugation associated with RT-qPCR, we detected untreated and treated wastewater samples obtained two treatment plants, together process around 85% from city. report Chile indicates that could be sensitive tool useful as predictive marker...
Abstract The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR were compatible with direct detection from nasopharyngeal swab samples. We show one tested is fully suggesting omission an step should be considered diagnosis.
Abstract We conducted a 16S rRNA nested PCR for the genus Ehrlichia and spp. with blood samples from 30 ill dogs in Chile. Phylogenetic analysis was performed by using groESL gene amplification. identified Anaplasma platys as 1 of etiologic agents canine ehrlichiosis.
The precursor group-specific antigen (pr55Gag) is central to HIV-1 assembly. Its expression alone sufficient assemble into virus-like particles. It also selects the genomic RNA for encapsidation and involved in several important virus-host interactions viral assembly restriction, making its synthesis essential aspects of replication. Here, we show that initiation translation mediated through both a cap-dependent an internal ribosome entry site (IRES)-mediated mechanisms. In support this...