A5081051634- Monoclonal and Polyclonal Antibodies Research
- Essential Oils and Antimicrobial Activity
- SARS-CoV-2 and COVID-19 Research
- Sesquiterpenes and Asteraceae Studies
- Virus-based gene therapy research
- Cellular transport and secretion
- Natural product bioactivities and synthesis
- Biochemical and Structural Characterization
- Computational Drug Discovery Methods
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Phytochemical compounds biological activities
- Research on Leishmaniasis Studies
- CAR-T cell therapy research
- Genetically Modified Organisms Research
- Seaweed-derived Bioactive Compounds
- Phytochemicals and Antioxidant Activities
- HER2/EGFR in Cancer Research
- Biotechnology and Related Fields
- Psidium guajava Extracts and Applications
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 detection and testing
- Bioactive Compounds and Antitumor Agents
- Psoriasis: Treatment and Pathogenesis
- T-cell and B-cell Immunology
Universidad Nacional de Asunción
2016-2024
University of Chile
2009-2015
Pontificia Universidad Católica de Chile
2013-2014
Millennium Institute on Immunology and Immunotherapy
2012
Summary A new paradigm has emerged relating the pathogenesis of rheumatoid arthritis (RA), focused on balance between T helper type 17 cells and regulatory (Tregs). In humans, both subpopulations depend transforming growth factor (TGF)-β for their induction, but in presence inflammatory cytokines, such as interleukin (IL)-6, generation Th17 is favoured. Tocilizumab a therapeutic antibody targeting IL-6 receptor (IL-6R), which demonstrated encouraging results RA. The aim this study was to...
The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. entry of virus into cell is one main targets inhibiting SARS-CoV-2 infection. Natural products are an important source therapeutic alternatives diseases. Pseudotyped viruses allow study viral inhibitors, and due their simplicity, they screening a large number candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 D614G spike glycoprotein test its ability infect...
Medicinal herbs have long been utilized to treat various diseases or relieve the symptoms of some ailments for extended periods. The present investigation demonstrates phytochemical profile, molecular docking, anti-Candida activity, and anti-viral activity Saussurea costus acetic acid extract. GC-MS analysis extract revealed presence 69 chemical compounds. compounds were alkaloids (4%), terpenoids (79%), phenolic hydrocarbons (7%), sterols (6%). Molecular docking was used study inhibitory...
Sorting nexin 17 ( SNX17 ) is an adaptor protein present in early endosomal antigen 1 EEA1 )‐positive sorting endosomes that promotes the efficient recycling of low‐density lipoprotein receptor‐related LRP1 to plasma membrane through recognition first NPxY motif cytoplasmic tail this receptor. The interaction with also regulates basolateral receptor from endosome BSE ). In contrast, megalin, which apically distributed polarized epithelial cells and localizes poorly ‐positive endosomes, does...
ApoER2 is a member of the low density-lipoprotein receptor (LDL-R) family. As for reelin, participates in neuronal migration during development as well synaptic plasticity and survival adult brain. A previous yeast two-hybrid screen showed that binding partner sorting nexin 17 (SNX17) - cytosolic adaptor protein regulates trafficking several membrane proteins endosomal pathway, including LRP1, P-selectin integrins. However, no further studies have been performed to investigate role SNX17...
The chemical composition of extracts (CEs) and essential oils (EOs) from Tetradenia riparia leaves, flower buds, stems was analyzed. Antiproliferative activity against tumor cell lines, NO production inhibition, antioxidant antiviral activities were assessed. CEs contained flavonoids, phenolic acids, coumarins, saturated fatty acids. EOs included monoterpenes, oxygenated sesquiterpenes, diterpenes. inhibition ranged 76 to 247 µg mL−1, antiproliferative exhibited GI50 between 20 >204 with...
Siddha medicine is one of the oldest medical systems in world and believed to have originated more than 10,000 years ago prevalent across ancient Tamil land. It undeniable that inhibitor preferences rise with increasing solubility water due considerations pertaining bioavailability ease which unabsorbed residues can be disposed of. In this study, we showed phytochemical discrimination Saussurea costus extracted at room temperature as a green extraction procedure. A total 48 compounds were...
Gastric cancer (GC) is the third most common cause of death worldwide. Natural killer cells play an important role in immune defense against transformed cells. They express activating receptor NKG2D, whose ligands belong to MIC and ULBP/RAET family. Although it well established that these are generally expressed tumors, association between their expression tumor gastric mucosa clinical parameters prognosis GC remains be addressed. In present study, MICA MICB was analyzed, by flow cytometry,...
The production of recombinant proteins in mammalian cell lines is one the most important areas biopharmaceutical industry. Viral transcriptional promoters are widely used to express lines. However, these susceptible silencing, thus limiting protein productivity. Some CpG islands can avoid silencing housekeeping genes; for that reason, they have been increase genes cells animal origin. In this study, we evaluated island promoter region β-actin gene Cricetulus griseous (Chinese hamster),...
Herpes simplex virus one is of the most prevalent pathogens worldwide. Strains resistant to current treatment have been reported, so it necessary search for new antiviral molecules. The common method quantify activity from natural products plaque reduction assay, a technically demanding method. In order provide simple alternative this method, we established procedure viral quantification by qPCR, and coupled with cytotoxicity evaluation system using resazurin. way, possible obtain both...
This study analyzes Paraguay’s biotechnology regulatory framework and its alignment with international standards amid biotechnological advancements. It also identifies areas of improvement for enhancing effectiveness. Through this work, we aim to provide a resource policymakers, stakeholders, researchers navigating regulation.
Phage display library technology is a common method to produce human antibodies. In this technique, the immunoglobulin variable regions are displayed in bacteriophage way that each filamentous virus displays product of single antibody gene on its surface. From collection different phages, it possible isolate recognizes specific targets. The most form which phage chain fragment format (scFv), requires assembly heavy and light gene. work, we describe simple efficient for scFv format. This...
Los anticuerpos constituyen un componente fundamental del sistema inmune, permitiendo el reconocimiento con alta especificidad y posterior destrucción de moléculas extrañas.Los monoclonales, producidos por la tecnología hibridoma, presentan desventajas para su uso en terapia humana debido a origen una especie diferente.La ingeniería genética posibilitó utilización los monoclonales terapias humanas, generando recombinantes terapéuticos.Así, se han transformado importante grupo fármacos;...
Severe Acute Respiratory Syndrome-Coronavirus-2 Virus (SARS-CoV-2) is responsible for Coronavirus Disease 2019 (COVID-19). A substantial number of SARS-CoV-2 infection cases have been reported during the pandemic, and vaccination coverage in some regions, particularly developing countries, remains very low. variants concern (VOCs) also emerged as most pressing public health issues. In this scenario, it crucial to know whether COVID-19 convalescent antibodies cross-neutralizing action against...
Herpes simplex virus 1 is one of the most prevalent pathogens worldwide. Resistant strains to current anti-viral treatment have been reported, requiring search for novel anti-virals. Using a qPCR method assess anti-herpetic activity from natural products, we analyzed 72 plant extracts El Salvador and identified eighteen methanolic with ≥ 75%. Anti-herpetic has not previously reported in fourteen plants (Euphorbia lancifolia, Piper tuberculatum, Cordia alliodora, Tecoma stans, Taraxacum...
Inflammatory processes, involving both innate and adaptive responses, are essential for controlling pathogens maintaining homeostasis. However, excessive inflammation can cause chronic conditions including autoimmune diseases, atherosclerosis, cancer. Alternative treatment options crucial, exploring plants with anti-inflammatory properties holds significance. The genus Baccharis (Asteraceae family) is widespread across the Americas traditionally used to treat various disorders, inflammatory...
There is a growing interest in the development of sustainable alternatives to use chemical pesticides for pest management agricultural systems. This research aimed isolate and characterize native strains Trichoderma spp. from different soils Paraguay using morphological molecular criteria. We processed plant soil samples eight commercial farms distributed departments isolated 14 monosporic isolates spp., obtaining two Department Alto Paraná (FCQ36 FCQ37), four Cordillera (FCQ42, FCQ43,...