A5010553674- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Celiac Disease Research and Management
- Monoclonal and Polyclonal Antibodies Research
- Mosquito-borne diseases and control
- Sesquiterpenes and Asteraceae Studies
- Essential Oils and Antimicrobial Activity
- T-cell and B-cell Immunology
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Computational Drug Discovery Methods
- Helicobacter pylori-related gastroenterology studies
- NF-κB Signaling Pathways
- Insect Pest Control Strategies
- Cytokine Signaling Pathways and Interactions
- Plant chemical constituents analysis
- Psidium guajava Extracts and Applications
- Microscopic Colitis
- Salivary Gland Disorders and Functions
- Dengue and Mosquito Control Research
- Diverse Scientific Research Studies
- RNA Interference and Gene Delivery
- Cervical Cancer and HPV Research
- Bioactive Compounds and Antitumor Agents
Universidad Nacional de Asunción
2016-2024
Instituto de Investigaciones en Ciencias de la Salud
2016
University of Chile
2009-2014
The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. entry of virus into cell is one main targets inhibiting SARS-CoV-2 infection. Natural products are an important source therapeutic alternatives diseases. Pseudotyped viruses allow study viral inhibitors, and due their simplicity, they screening a large number candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 D614G spike glycoprotein test its ability infect...
Toll-like receptor (TLR) 2, a type I membrane that plays key role in innate immunity, recognizes conserved molecules pathogens, and triggering an inflammatory response. It has been associated with autoimmune diseases. Soluble TLR2 (sTLR2) variants have identified human body fluids, the ectodomain can negatively regulate activation by behaving as decoy receptor. sTLR2 generation does not involve alternative splicing mechanisms, indicating this process might post-translational modification of...
In 2019-2020, dengue virus (DENV) type 4 emerged to cause the largest DENV outbreak in Paraguay's history. This study sought characterize relative other acute illness cases and use phylogenetic analysis understand outbreak's origin. Individuals with an (≤7 days) were enrolled tested for nonstructural protein 1 (NS1) viral RNA by real-time RT-PCR. Near-complete genome sequences obtained from 62 DENV-4 positive samples. From January 2019 March 2020, 799 participants enrolled: 253 (14 severe...
Airway inflammation is a common condition where glucocorticoids (GC) are well-established therapy. It has been demonstrated that GC stimulate components of innate immunity. Specifically, up-regulate TLR2 expression and activation upon inflammatory stimuli; however, little known about the signalling involved in this process. To determine mechanism by which dexamethasone modulates TLR2-induced cytokine production pathway was monitored lung epithelial cell line exposed to synthetic agonist,...
Background Dengue is the most common vector-borne viral disease worldwide. Most cases are mild, but some evolve into severe dengue (SD), with high lethality. Therefore, it important to identify biomarkers of improve outcomes and judiciously utilize resources. Methods/Principal findings One hundred forty-five confirmed (median age, 42; range <1–91 years), enrolled from February 2018 March 2020, were selected an ongoing study suspected arboviral infections in metropolitan Asunción,...
Herpes simplex virus one is of the most prevalent pathogens worldwide. Strains resistant to current treatment have been reported, so it necessary search for new antiviral molecules. The common method quantify activity from natural products plaque reduction assay, a technically demanding method. In order provide simple alternative this method, we established procedure viral quantification by qPCR, and coupled with cytotoxicity evaluation system using resazurin. way, possible obtain both...
ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Ferreira S, Masi J, Giménez V, et al. Effect of gluten-free diet on levels soluble CD14 and lipopolysaccharide-binding protein in adult patients with celiac disease. Central European Journal Immunology. 2021;46(2):225-230. doi:10.5114/ceji.2021.107012. APA Ferreira, S., Masi, J., Giménez, V., Carpinelli, M., Laterza, O., & Hermoso, M. (2021). Immunology, 46(2), 225-230. https://doi.org/10.5114/ceji.2021.107012...
Los anticuerpos constituyen un componente fundamental del sistema inmune, permitiendo el reconocimiento con alta especificidad y posterior destrucción de moléculas extrañas.Los monoclonales, producidos por la tecnología hibridoma, presentan desventajas para su uso en terapia humana debido a origen una especie diferente.La ingeniería genética posibilitó utilización los monoclonales terapias humanas, generando recombinantes terapéuticos.Así, se han transformado importante grupo fármacos;...
Severe Acute Respiratory Syndrome-Coronavirus-2 Virus (SARS-CoV-2) is responsible for Coronavirus Disease 2019 (COVID-19). A substantial number of SARS-CoV-2 infection cases have been reported during the pandemic, and vaccination coverage in some regions, particularly developing countries, remains very low. variants concern (VOCs) also emerged as most pressing public health issues. In this scenario, it crucial to know whether COVID-19 convalescent antibodies cross-neutralizing action against...
Herpes simplex virus 1 is one of the most prevalent pathogens worldwide. Resistant strains to current anti-viral treatment have been reported, requiring search for novel anti-virals. Using a qPCR method assess anti-herpetic activity from natural products, we analyzed 72 plant extracts El Salvador and identified eighteen methanolic with ≥ 75%. Anti-herpetic has not previously reported in fourteen plants (Euphorbia lancifolia, Piper tuberculatum, Cordia alliodora, Tecoma stans, Taraxacum...
Inflammatory processes, involving both innate and adaptive responses, are essential for controlling pathogens maintaining homeostasis. However, excessive inflammation can cause chronic conditions including autoimmune diseases, atherosclerosis, cancer. Alternative treatment options crucial, exploring plants with anti-inflammatory properties holds significance. The genus Baccharis (Asteraceae family) is widespread across the Americas traditionally used to treat various disorders, inflammatory...
El virus del papiloma humano (VPH) es causa necesaria pero no suficiente cáncer de cuello uterino (CCU), cuarta muerte en mujeres a nivel mundial. En nuestro país, la incidencia y mortalidad CCU 30,6 16,7 por 100.000 mujeres, respectivamente; siendo un problema salud pública. Los VPH son alto riesgo oncogénico (VPH-AR), asociados neoplasia intraepitelial cervical (NIC) bajo (VPH-BR), relacionados verrugas genitales. objetivo fue comparar dos pruebas diagnósticas detección VPH, PCR tiempo...
Systemic lupus erythematosus (SLE) is a complex autoimmune disease characterized by heterogeneous clinical picture that makes the diagnosis and follow-up of these patients difficult. This study aimed to identify correlations between clinical, immunological, genetic biomarkers manifestations in SLE. A retrospective data from medical records immunological studies SLE Paraguay was carried out. descriptive analysis performed based on type variable. Human leukocyte antigen (HLA) allele...
Las plantas del género Baccharis son empleadas en la medicina popular de Sudamérica para el alivio y tratamiento diversas dolencias, entre ellas aquellas que cursan con procesos inflamatorios, por lo constituyen una fuente potencial compuestos actividad sobre sistema inmune. La creciente necesidad nuevos fármacos inmunomoduladora ha redirigido atención a las uso medicinal tradicional. Diversos estudios revelan especies forman parte este género, destacándose anti-inflamatoria. Entre más...
Genetic and nongenetic factors are involved in the pathogenesis of immune-mediated inflammatory diseases (IMIDs). The best-known genetic factor for susceptibility to IMIDs is human leukocyte antigen (HLA). aim present study was evaluate association HLA class II genes with risk systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), sclerosis (SSc) Paraguayan population. We included 254 patients (101 SLE, 103 RA, 50 SSc) healthy controls. haplotypes five corresponding their...
Systemic exposure to bacterial components like lipopolysaccharide (LPS) is among the non-genetic factors that could be involved in onset or progression of systemic lupus erythematosus (SLE). Lipopolysaccharide-binding protein (LBP) participates recognition LPS and inflammatory response. Here, we investigated LBP SLE patients its relationship with disease activity phenotypes.Eighty-one adult from IMID-PY biobank (Paraguay) were included study. The clinical laboratory variables used determine...
<h3>Background</h3> Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease that affects lacrimal and salivary glands, whereby SS-patients complain of eye mouth dryness. Salivary-acinar-cells display alterations in cell polarity affecting the localization function proteins involved regulated exocytosis. In salivary-glands (SG) from SS-patients, levels mRNA protein for VAMP8, STX4 STX3 were altered. STX4, STX3, SNAP-23 VAMP8 relocated apical to basal region acinar-cells....
<h3>Background</h3> Systemic Lupus Erythematosus (SLE) is a complex autoimmune disease characterized by very heterogeneous clinical picture that makes diagnosis and follow-up of these patients difficult. <h3>Objectives</h3> Identify correlations between clinical, immunological genetic biomarkers with manifestations in SLE Paraguayan patients. <h3>Methods</h3> Retrospective study data from medical records studies Paraguay. A descriptive analysis was performed based on the type variable. HLA...
El virus chikungunya (CHIKV) es un alfavirus cuya infección provoca una enfermedad caracterizada principalmente por fiebre y dolores articulares/musculares. Entre 25-50% de las infecciones se presentan con crónica que puede durar meses a años. primer brote CHIKV en Paraguay corresponde al año 2015, siendo el último 2022/2023. Diversos candidatos vacunales contra encuentran diferentes etapas desarrollo, e incluso recientemente (noviembre/2023) fue aprobada la primera vacuna llamada VLA1553...
Las plantas de uso en medicina tradicional constituyen una fuente importante compuestos con actividad inmunomoduladora; entre ellas las especies del género Baccharis, conocidas popularmente como âJaguareteka´aâ nuestro paÃs, son ampliamente empleadas. En este estudio se evaluó la inmunomoduladora extractos metanólicos tres Baccharis (B. trimera, B. notosergilay punctulata) sobre proliferación células mononucleares humanas sangre periférica. Los estudiadas estimularon...