A5001207932- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Dengue and Mosquito Control Research
- Viral Infections and Outbreaks Research
- Immune responses and vaccinations
- Long-Term Effects of COVID-19
- HIV Research and Treatment
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
Emory University
2020-2024
Broad testing for respiratory viruses among persons under investigation (PUIs) SARS-CoV-2 has been performed inconsistently, limiting our understanding of alternative viral infections and coinfections in these patients. RNA metagenomic next-generation sequencing (mNGS) offers an agnostic tool the detection both other PUIs. Here, we used mNGS to assess frequencies RT-PCR-negative PUIs ( n = 30) RT-PCR-positive 45).
Acute febrile illness is a common problem managed by clinicians and health systems globally, particularly in the Tropics. In many regions, malaria leading potentially deadly cause of fever; however, myriad alternative etiologies exist. Identifying fever allows optimal management, but this depends on factors including thorough knowledge circulating infections. Arboviruses such as dengue (DENV) may be underdiagnosed sub-Saharan Africa where major focus. We examined cases western Cameroon that...
In 2019-2020, dengue virus (DENV) type 4 emerged to cause the largest DENV outbreak in Paraguay's history. This study sought characterize relative other acute illness cases and use phylogenetic analysis understand outbreak's origin. Individuals with an (≤7 days) were enrolled tested for nonstructural protein 1 (NS1) viral RNA by real-time RT-PCR. Near-complete genome sequences obtained from 62 DENV-4 positive samples. From January 2019 March 2020, 799 participants enrolled: 253 (14 severe...
Eastern equine encephalitis virus (EEEV) is a relatively little-studied alphavirus that can cause devastating viral encephalitis, potentially leading to severe neurological sequelae or death. Although case numbers have historically been low, outbreaks increasing in frequency and scale since the 2000 s. It critical investigate EEEV evolutionary patterns, especially within human hosts, understand patterns of emergence, host adaptation, within-host evolution. To this end, we obtained...
Abstract We used metagenomic next-generation sequencing (mNGS) to assess the frequencies of alternative viral infections in SARS-CoV-2 RT-PCR negative persons under investigations (PUIs) (n=30) and co-infections positive PUIs (n=45). mNGS identified both that were not detected by routine clinical workup.
Abstract Background SARS-CoV-2 mutations conferring escape from neutralizing antibodies can arise in immunocompromised patients with prolonged infection, but the conditions that facilitate immune are still not fully understood. Methods We characterized endogenous responses, within-host evolution, and autologous neutralization of viral variants arose five infection B cell deficiencies. Results In two treated monoclonal antibody bamlanivimab, resistance to serum early persisted for several...
Standard molecular detection of many pathogens, in particular RNA viruses, requires appropriate handling the field for preserving quality sample until processing. This could be challenging remote tropical areas. Dengue virus (DENV), chikungunya (CHIKV), and Zika (ZIKV) are prominent among causes fever tropics. We aimed to test stability arboviral contrived dried blood spots prepared on Whatman 903 Protein saver cards as a means collection storage. were able detect DENV, CHIKV, ZIKV by...
Abstract Dengue virus (DENV) is a significant public health concern in Colombia, with increased transmission of DENV type 1 (DENV-1) the departments Risaralda and Valle del Cauca Central-West region country following large outbreak 2019. However, little known about source, genetic diversity, evolution circulating viruses. We obtained plasma samples from individuals acute infection analyzed DENV-1 phylodynamics, phylogeography. found that most viruses belonged to genotype V, phylogenetic...
Abstract Background Broad testing for respiratory viruses among persons under investigation (PUI) SARS-CoV-2 is performed inconsistently, limiting our understanding of alternative infections and co-infections in these patients. Here, we used unbiased metagenomic next-generation sequencing (mNGS) to assess the frequencies 1) viral RT-PCR negative PUIs 2) positive PUIs. Methods A convenience sample set was selected from who were tested Emory Healthcare system during first 2 months pandemic...