A5048952101- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Poxvirus research and outbreaks
- Malaria Research and Control
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Bacillus and Francisella bacterial research
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Yersinia bacterium, plague, ectoparasites research
- Viral gastroenteritis research and epidemiology
- Zoonotic diseases and public health
- Insect symbiosis and bacterial influences
- Viral Infections and Immunology Research
- Rabies epidemiology and control
- Dengue and Mosquito Control Research
- Global Maternal and Child Health
- Respiratory viral infections research
- Animal Virus Infections Studies
- Vector-borne infectious diseases
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
University of Wisconsin–Madison
2016-2025
University of Wisconsin Health
2022-2025
Universidad Nacional de Colombia
2018-2025
Abbott (United States)
2022-2025
Abbott Fund
2025
United States University
2024
Monash University
2023
Bioversity International
2022
University of Wisconsin System
2010-2020
Madison Group (United States)
2019
The recent emergence of B.1.1.529, the Omicron variant
Abstract Infection with Asian-lineage Zika virus (ZIKV) has been associated Guillain–Barré syndrome and fetal abnormalities, but the underlying mechanisms remain poorly understood. Animal models of infection are thus urgently needed. Here we show that rhesus macaques susceptible to by an ZIKV closely related strains currently circulating in Americas. Following subcutaneous inoculation, RNA is detected plasma 1 day post (d.p.i.) all animals ( N =8, including 2 pregnant animals), also present...
Abstract Zika virus (ZIKV) is causing an explosive outbreak of febrile disease in the Americas. There are no effective antiviral therapies or licensed vaccines for this and mosquito control strategies have not been adequate to contain virus. A promising candidate arbovirus prevention relies on introduction intracellular bacterium Wolbachia into Aedes aegypti mosquitoes. This primarily has proposed as a tool dengue (DENV) transmission; however, evidence suggests infections confer protection...
Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as fever, which often is accompanied by maculopapular rash, headache, myalgia. During the current outbreak in South America, ZIKV infection during pregnancy has been hypothesized to cause microcephaly other diseases. The detection of fetal brain tissue supports this hypothesis. Because human infections with historically have remained sporadic and, until recently, limited small-scale epidemics, neither...
Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there an urgent need for the discovery of agents capable inhibiting its viral replication deleterious effects. Chloroquine widely used as antimalarial drug, anti-inflammatory agent, it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits ZIKV Vero cells, human brain microvascular endothelial neural stem mouse...
Infection with Zika virus (ZIKV) is associated human congenital fetal anomalies. To model outcomes in nonhuman primates, we administered Asian-lineage ZIKV subcutaneously to four pregnant rhesus macaques. While non-pregnant animals a previous study contemporary the current report clear viremia within 10–12 days, maternal was prolonged 3 of 4 pregnancies. Fetal head growth velocity last month gestation determined by ultrasound assessment circumference decreased comparison biparietal diameter...
During 2012, 2013 and 2015, we collected small mammals within 25 km of the town Boende in Tshuapa Province, Democratic Republic Congo. The prevalence monkeypox virus (MPXV) this area is unknown; however, cases human infection were previously confirmed near these collection sites. Samples from 353 (rodents, shrews, pangolins, elephant a potamogale, hyrax). Some rodents shrews captured houses where have recently been identified, but most trapped forests agricultural areas villages. Real-time...
Background New approaches to preventing chikungunya virus (CHIKV) are needed because current methods limited controlling mosquito populations, and they have not prevented the invasion of this into new locales, nor been sufficient control upon arrival. A promising candidate for arbovirus prevention relies on introduction intracellular bacterium Wolbachia Aedes aegypti mosquitoes. This primarily has proposed as a tool dengue (DENV) transmission; however, evidence suggests infections confer...
Significance Immunity induced by the first-generation COVID-19 vaccines may not provide effective and durable protection, either due to waning immunity or poor antibody cross-reactivity new variants. Typically, T cells recognize conserved nonmutable viral epitopes development of cell–based might broad SARS-CoV-2 In this study, we show that adjuvanted spike protein–based experimental elicited potent respiratory systemic CD4 CD8 cell memory protected against SARS-CoV-2, in absence...
Chikungunya virus (CHIKV) is the mosquito-borne alphavirus that etiologic agent of massive outbreaks arthralgic febrile illness recently affected millions people in Africa and Asia. The only CHIKV vaccine has been tested humans, strain 181/clone 25, a live-attenuated derivative Southeast Asian human isolate AF15561. was immunogenic phase I II clinical trials; however, it induced transient arthralgia 8% vaccinees. There are five amino acid differences between its parent, as well synonymous...
Multiple monkeypox virus (MPXV) animal models have been discussed in previous studies, but no small models, nor most non-human primate demonstrated the protracted asymptomatic incubation phase seen systemic human orthopoxvirus illness. Herein, we characterize a black-tailed prairie dog (PD) ( Cynomys ludovicianus ) model of infection, via intranasal and intradermal exposures, with two MPXV clades. Daily observations animals were made (food consumption, general symptoms, disease...
Chikungunya virus (CHIKV) is a reemerging mosquito-borne pathogen that has recently caused devastating urban epidemics of severe and sometimes chronic arthralgia. As with most other viral diseases, control relies on reducing mosquito populations their contact people, which been ineffective in locations. Therefore, vaccines remain the best strategy to prevent vector-borne diseases. Ideally, for diseases resource-limited countries should combine low cost single dose efficacy, yet induce rapid...
Background Zika virus (ZIKV; Flaviviridae, Flavivirus) was declared a public health emergency of international concern by the World Health Organization (WHO) in February 2016, because evidence linking infection with ZIKV to neurological complications, such as Guillain-Barre Syndrome adults and congenital birth defects including microcephaly developing fetus. Because development vaccine is top research priority genetic antigenic variability many RNA viruses limits effectiveness vaccines,...
Monkeypox (MPX) is a zoonotic disease endemic in Central and West Africa caused by virus (MPXV), the most virulent Orthopoxvirus affecting humans since eradication of Variola (VARV). Many aspects MPXV transmission cycle, including natural host virus, remain unknown. African rope squirrels (Funisciurus spp.) are considered potential reservoirs MPXV, as serosurveillance data has confirmed circulation these rodent species [1,2]. In order to understand tissue tropism clinical signs associated...
Abstract Mouse and nonhuman primate models now serve as useful platforms to study Zika virus (ZIKV) pathogenesis, candidate therapies, vaccines, but they rely on needle inoculation of virus: the effects mosquito-borne infection disease outcome have not been explored in these models. Here we show that via mosquito bite delays ZIKV replication peak viral loads rhesus macaques. Importantly, mosquito-infected animals tissue distribution was limited hemolymphatic tissues, female reproductive...
Congenital Zika virus (ZIKV) infection impacts fetal development and pregnancy outcomes. We infected a pregnant rhesus macaque with Puerto Rican ZIKV isolate in the first trimester. The was complicated by preterm premature rupture of membranes (PPROM), intraamniotic bacterial demise 49 days post (gestational day 95). Significant pathology at maternal-fetal interface included acute chorioamnionitis, placental infarcts, leukocytoclastic vasculitis myometrial radial arteries. RNA disseminated...
Abstract Despite the development and deployment of antibody vaccine countermeasures, rapidly-spreading SARS-CoV-2 variants with mutations at key antigenic sites in spike protein jeopardize their efficacy. The recent emergence B.1.1.529, Omicron variant1,2, which has more than 30 protein, raised concerns for escape from protection by vaccines therapeutic antibodies. A test potential countermeasures against B.1.1.529 is activity pre-clinical rodent models respiratory tract disease. Here, using...
Congenital Zika virus (ZIKV) infection was first linked to birth defects during the American outbreak in 2015/2016. It has been proposed that mutations unique Asian/American-genotype explain, at least part, ability of Asian/American ZIKV cause congenital syndrome (CZS). Recent studies identified infecting humans arose coincident with French Polynesia and were stably maintained subsequent spread Americas. Here we show African can infect harm fetuses S139N substitution associated is not...