A5055870287- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Virology and Viral Diseases
- Hepatitis C virus research
- Herpesvirus Infections and Treatments
- Malaria Research and Control
- Long-Term Effects of COVID-19
- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- COVID-19 epidemiological studies
- Bioactive Natural Diterpenoids Research
- Infectious Encephalopathies and Encephalitis
- Global Maternal and Child Health
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
Universidade Federal do Rio de Janeiro
2016-2025
Brazilian Agricultural Research Corporation
2025
National Institute of Science and Technology for Structural Biology and Bioimaging
2020-2022
Instituto Nacional do Câncer
2017
Fundação de Medicina Tropical
2017
Instituto Biológico
2017
KU Leuven
2015
Rega Institute for Medical Research
2015
Universidade do Estado do Rio de Janeiro
2014
Columbia University
2009-2013
Zika virus tested in human brain organoids The pernicious and resilient Aedes mosquito is rapidly spreading (ZIKV) through the Americas. ZIKV infection mostly causes mild disease, but some patients, nervous system involvement indicated. A particular worry an observed correlation between of mothers first trimester pregnancy microcephaly newborns. Garcez et al. effects compared with dengue on neural stem cells grown as organoids. targeted cells, reduced their size viability vitro, caused...
Brazil currently has one of the fastest-growing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemics in world. Because limited available data, assessments impact nonpharmaceutical interventions (NPIs) on this virus spread remain challenging. Using a mobility-driven transmission model, we show that NPIs reduced reproduction number from >3 to 1 1.6 São Paulo and Rio de Janeiro. Sequencing 427 new genomes analysis geographically representative genomic dataset identified >100...
The yellow fever virus (YFV) epidemic in Brazil is the largest decades. recent discovery of YFV Brazilian Aedes species mosquitos highlights a need to monitor risk reestablishment urban transmission Americas. We use suite epidemiological, spatial, and genomic approaches characterize transmission. show that age sex distribution human cases characteristic sylvatic Analysis combined with genomes generated locally reveals an early phase spatial expansion toward previously YFV-free areas,...
Purpose To document the imaging findings associated with congenital Zika virus infection as found in Instituto de Pesquisa Campina Grande State Paraiba (IPESQ) northeastern Brazil, where has been particularly severe. Materials and Methods From June 2015 to May 2016, 438 patients were referred IPESQ for rash occurring during pregnancy or suspected fetal central nervous system abnormality. Patients who underwent at included, well those documented fluid tissue (n = 17, confirmed cohort) brain...
Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there an urgent need for the discovery of agents capable inhibiting its viral replication deleterious effects. Chloroquine widely used as antimalarial drug, anti-inflammatory agent, it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits ZIKV Vero cells, human brain microvascular endothelial neural stem mouse...
Abstract In this study, we report the sequencing of 180 new viral genomes obtained from different municipalities state Rio de Janeiro April to December 2020. We identified a novel lineage SARS-CoV-2, originated B.1.1.28, distinguished by five single-nucleotide variants (SNVs): C100U, C28253U, G28628U, G28975U, and C29754U. The SNV G23012A (E484K), in receptor-binding domain Spike protein, was widely spread across samples. This mutation previously associated with escape neutralizing...
Background The genetic differences among HIV-1 subtypes may be critical to clinical management and drug resistance surveillance as antiretroviral treatment is expanded regions of the world where diverse non-subtype-B viruses predominate. Methods Findings To assess impact subtype on distribution mutations in protease reverse transcriptase, a binomial response model using explanatory variables was used analyze large compiled dataset sequences. Non-subtype-B sequences from 3,686 persons with...
Isolates of foot-and-mouth disease virus (FMDV) exist as complex mixtures variants. Two different serotype O1 Campos preparations that we examined contained two variants with distinct plaque morphologies on BHK cells: a small, clear-plaque replicates in and CHO cells, large, turbid-plaque only grows cells. cDNAs encoding the capsids these were inserted into genome-length FMDV type A12 infectious cDNA used to produce chimeric viruses exhibited phenotype original Analyses viruses, hybrids...
To study the prevalence of HIV drug resistance mutations and subtype distribution in a Brazilian drug-naive population. Asymptomatic, HIV-1-infected individuals were targeted 13 voluntary counseling testing centers spread around country.Plasma viral RNA was extracted from 535 HIV-1-positive subjects. Protease (PR) reverse transcriptase (RT) genomic regions sequenced for determination analysis mutations.Eight samples (2.24 %) showed primary related to protease inhibitor (PI) resistance, eight...
To identify a new circulating recombinant form (CRF) of HIV-1 comprising two subtypes in the southern region Brazil, B and C.A total 152 HIV-positive patients followed at hospitals Brazil had their viral pol genes isolated by reverse transcriptase-polymerase chain reaction (PCR) from plasma. PCR products were sequenced phylogenetically analysed using subtype reference sequences. Six full-length C viruses previously described as 'pure' strains included analysis. Sequences suggestive...
Abstract Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research revealed that astrocytes are targets of ZIKV. However, the consequences ZIKV infection, especially this cell type, remain largely unknown, particularly considering integrative studies aiming understand crosstalk among key cellular mechanisms fates involved in neurotoxicity virus. Here, infection iPSC-derived presented. Our results show ROS imbalance, mitochondrial...
Zika virus (ZIKV) has been associated to central nervous system (CNS) harm, and was detected in the brain cerebrospinal fluids of microcephaly meningoencephalitis cases. However, mechanism by which reaches CNS is unclear. Here, we addressed effects ZIKV replication human microvascular endothelial cells (HBMECs), as an vitro model blood barrier (BBB), evaluated extravasation BBB integrity a vivo mouse experimental model. HBMECs were productively infected African Brazilian strains (ZIKVMR766...
Abstract Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human development are still not fully understood. Here we describe alterations in neurospheres derived from induced pluripotent stem (iPS) cells infected strain that is circulating Brazil. Combining proteomics mRNA transcriptional profiling, over 500 proteins genes Brazilian infection were found be differentially expressed. These provide an...
Yellow fever virus (YFV) is a member of the Flaviviridae family. In Brazil, yellow (YF) cases have increased dramatically in sylvatic areas neighboring urban zones last few years. Because high lethality rates associated with infection and absence any antiviral treatments, it essential to identify therapeutic options respond YFV outbreaks. Repurposing clinically approved drugs represents fastest alternative discover antivirals for public health emergencies. Other Flaviviruses, such as Zika...
Zika virus infections and suspected microcephaly cases have been reported in Angola since late 2016, but no data are available about the origins, epidemiology, diversity of virus. We aimed to investigate emergence circulation Angola.Diagnostic samples collected by Angolan Ministry Health as part routine arboviral surveillance were tested real-time reverse transcription PCR Instituto Nacional de Investigação em Saúde (Ministry Health, Luanda, Angola). To identify further positive for...
Zika virus (ZIKV) causes significant public health concerns because of its association with congenital malformations, neurological disorders in adults, and, more recently, death. Considering the necessity to mitigate ZIKV-associated diseases, antiviral interventions are an urgent necessity. Sofosbuvir, a drug clinical use against hepatitis C (HCV), is among FDA-approved substances endowed anti-ZIKV activity. In this work, we further investigated vivo activity sofosbuvir ZIKV. Neonatal Swiss...