Joilson Xavier
A5026752634- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Zoonotic diseases and public health
- Virology and Viral Diseases
- SARS-CoV-2 detection and testing
- Insect symbiosis and bacterial influences
- COVID-19 epidemiological studies
- Viral Infections and Outbreaks Research
- Animal Virus Infections Studies
- Genomics and Phylogenetic Studies
- Fish biology, ecology, and behavior
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Vibrio bacteria research studies
- Plant Virus Research Studies
- Primate Behavior and Ecology
- Vector-borne infectious diseases
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- HIV Research and Treatment
- Dengue and Mosquito Control Research
- vaccines and immunoinformatics approaches
- Advanced biosensing and bioanalysis techniques
Universidade Federal de Minas Gerais
2019-2024
Fundação Oswaldo Cruz
2018-2024
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
2021-2023
Ezequiel Dias Foundation
2021-2023
Centro Universitário de Belo Horizonte
2023
Pan American Health Organization (Brasil)
2023
Università Campus Bio-Medico
2023
Universidade de São Paulo
2023
Universidade Estadual de Campinas (UNICAMP)
2023
Instituto Biológico
2022
The yellow fever virus (YFV) epidemic in Brazil is the largest decades. recent discovery of YFV Brazilian Aedes species mosquitos highlights a need to monitor risk reestablishment urban transmission Americas. We use suite epidemiological, spatial, and genomic approaches characterize transmission. show that age sex distribution human cases characteristic sylvatic Analysis combined with genomes generated locally reveals an early phase spatial expansion toward previously YFV-free areas,...
Abstract The high numbers of COVID-19 cases and deaths in Brazil have made Latin America an epicentre the pandemic. SARS-CoV-2 established sustained transmission early pandemic, but important gaps remain our understanding virus dynamics at a national scale. We use 17,135 near-complete genomes sampled from 27 Brazilian states bordering country Paraguay. From March to November 2020, we detected co-circulation multiple viral lineages that were linked importations (predominantly Europe). After...
Arbovirus infections in Brazil, including yellow fever, dengue, zika, and chikungunya, result considerable morbidity mortality are pressing public health concerns. However, our understanding of these outbreaks is hampered by the limited availability genomic data. In this study, we investigated genetic diversity spatial distribution YFV during current outbreak analyzing data from areas southeastern Brazil not covered other previous studies. To gain insights into routes introduction...
Zika virus infections and suspected microcephaly cases have been reported in Angola since late 2016, but no data are available about the origins, epidemiology, diversity of virus. We aimed to investigate emergence circulation Angola.Diagnostic samples collected by Angolan Ministry Health as part routine arboviral surveillance were tested real-time reverse transcription PCR Instituto Nacional de Investigação em Saúde (Ministry Health, Luanda, Angola). To identify further positive for...
Background Since its first detection in the Caribbean late 2013, chikungunya virus (CHIKV) has affected 51 countries Americas. The CHIKV epidemic Americas was caused by CHIKV-Asian genotype. In August 2014, local transmission of genotype detected Brazilian Amazon region. However, a distinct lineage, CHIKV-East-Central-South-America (ECSA)-genotype, nearly simultaneously Feira de Santana, Bahia state, northeast Brazil. genomic diversity and dynamics region remains poorly understood despite...
Summary Genomic surveillance has become a useful tool for better understanding virus pathogenicity, origin and spread. Obtaining accurately assembled, complete viral genomes directly from clinical samples is still challenging. Here, we describe three protocols using unique primer set designed to recover long reads of SARS-CoV-2 total RNA extracted samples. This protocol useful, accessible adaptable laboratories with varying resources access distinct sequencing methods: Nanopore, Illumina...
Abstract The coronavirus disease 2019 (COVID-19) epidemic in Brazil was driven mainly by the spread of Gamma (P.1), a locally emerged variant concern (VOC) that first detected early January 2021. This estimated to be responsible for more than 96 per cent cases reported between and June 2021, being associated with increased transmissibility severity, reduction neutralization antibodies effectiveness treatments or vaccines, diagnostic detection failure. Here we show that, following several...
We used nanopore sequencing and phylogenetic analyses to identify a cosmopolitan genotype of dengue virus serotype 2 that was isolated from 56-year-old male patient the state Goiás in Brazil. The emergence Brazil will require risk assessment surveillance reduce epidemic potential.
Yellow fever disease is considered a re-emerging major health issue which has caused recent outbreaks with high number of deaths. Tropical countries, mainly African and South American, are the most affected by outbreaks. Despite availability an attenuated vaccine, its use limited for some groups such as pregnant nursing women, immunocompromised immunosuppressed patients, elderly people >65 years, infants <6 months patients biological disorders like thymus disorders. In order to achieve new...
Zika virus (ZIKV) has caused an explosive epidemic linked to severe clinical outcomes in the Americas. As of June 2018, 4,929 ZIKV suspected infections and 46 congenital syndrome cases had been reported Manaus, Amazonas, Brazil. Although Manaus is a key demographic hub Amazon region, little known about there, terms both transmission viral genetic diversity. Using portable genome sequencing, we generated 59 genomes Manaus. Phylogenetic analyses indicated multiple introductions from...
Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field classroom initiatives for the monitoring of DENV Brazil, generating 227 novel genome sequences DENV1-2 from 85 municipalities (2015-2019). This equated to an over 50% increase number genomes available databases. both phylogenetic epidemiological models retrospectively reconstructed recent...
The spread of Chikungunya virus is a major public health concern in the Americas. There were >120,000 cases and 51 deaths 2023, which 46 occurred Paraguay. Using suite genomic, phylodynamic, epidemiologic techniques, we characterized ongoing large chikungunya epidemic
Despite the considerable morbidity and mortality of yellow fever virus (YFV) infections in Brazil, our understanding disease outbreaks is hampered by limited viral genomic data. Here, through a combination phylogenetic epidemiological models, we reconstructed recent transmission history YFV within different epidemic seasons Brazil. A suitability index based on highly domesticated Aedes aegypti was able to capture seasonality reported human infections. Spatial modeling revealed spatial...
This report provides a detailed overview of the resurgence DENV-3 in state Minas Gerais, Brazil, which is concerning scenario context dengue, mosquito-borne viral disease. Historically, Brazil has grappled with dengue epidemics caused primarily by DENV-1 and DENV-2 serotypes. However, 2023, significant shift this pattern was observed as made notable resurgence. characterized increase cases within country region Americas. Given absence sustained circulation previous years, situation poses...
The emergence of chikungunya virus (CHIKV) has raised serious concerns due to the virus' rapid dissemination into new geographic areas and clinical features associated with infection. To better understand CHIKV dynamics in Rio de Janeiro, we generated 11 near-complete genomes by means real-time portable nanopore sequencing isolates obtained directly from samples. Our phylogenetic reconstructions indicated circulation East-Central-South-African (ECSA) lineage Janeiro. Time-measured analysis...
Zika virus (ZIKV) is an arthropod-borne (arbovirus) in the Flavivirus genus of Flaviviridae family. Since large outbreaks French Polynesia 2013-2014 and Brazil 2015, ZIKV has been considered a new public health threat. Similar to other related flavivirus, associated with mild self-limiting symptoms such as rash, pruritus, prostration, headache, arthralgia, myalgia, conjunctivitis, lower back pain and, when present, short-term low grade fever. In addition, implicated neurological...
The recent emergence of a coronavirus (SARS-CoV-2), first identified in the Chinese city Wuhan December 2019, has had major public health and economic consequences. Although 61,888 confirmed cases were reported Brazil by 28 April 2020, little is known about SARS-CoV-2 epidemic this country. To better understand second most populous state southeast - Minas Gerais (MG) we sequenced 40 complete genomes from MG examined epidemiological data three Brazilian states. Both genome analyses...
Abstract The emergence and reemergence of mosquito-borne diseases in Brazil such as yellow fever, zika, chikungunya, dengue have had serious impacts on public health. Concerns been raised due to the rapid dissemination chikungunya virus across country since its first detection 2014 Northeast Brazil. In this work, we carried out on-site training activities genomic surveillance partnership with National Network Public Health Laboratories that led generation 422 genomes from 12 Brazilian states...
Background The transmission patterns and genetic diversity of dengue virus (DENV) circulating in Africa remain poorly understood. Circulation the DENV serotype 1 (DENV1) Angola was detected 2013, while 2 (DENV2) 2018. Here, we report results from molecular genomic investigations conducted at Ministry Health national reference laboratory (INIS) on suspected cases between January 2017 February 2019. Methods A total 401 serum samples were collected 13 18 provinces Angola. Of those, 351 had...
Background: West Nile virus (WNV) was first sequenced in Brazil 2019, when it isolated from a horse the Espírito Santo state. Despite multiple studies reporting serological evidence suggestive of past circulation since 2004, WNV remains low priority for surveillance and public health, such that much is still unknown about its genomic diversity, evolution, transmission country. Methods: A combination diagnostic assays, nanopore sequencing, phylogenetic inference, epidemiological modeling are...
Dengue fever is among the most significant public health concerns in Brazil. To date, highest number of notifications Americas has been reported Brazil, with cases accounting for a total 3,418,796 as mid-December 2022. Furthermore, northeastern region Brazil registered second-highest incidence Due to alarming epidemiological scenario, this study, we used combination portable whole-genome sequencing, phylodynamic, and analyses reveal novel DENV-1 genotype V clade persistence DENV-2 III...
The Northeast region of Brazil registered the second-highest incidence proportion Chikungunya fever in 2019. In that year, an outbreak consisting patients presenting with febrile disease associated joint pain was reported by public primary health care service city Natal, state Rio Grande do Norte, March At first, aetiological agent undetermined. Since much is still unknown about chikungunya virus' (CHIKV) genomic diversity and evolutionary history this northeasternmost state, we used a...
Abstract Paraguay has been severely affected by emergent Zika and chikungunya viruses, dengue virus is endemic. To learn more about the origins of genetic diversity epidemiologic history these viruses in Paraguay, we deployed portable sequencing technologies to strengthen genomic surveillance determine evolutionary epidemic arthropod-borne (arboviruses). Samples stored at National Central Laboratory were sequenced subjected phylogenetic analysis. Among 33 genomes generated, identified 2...
Yellow fever virus (YFV) causes a clinical syndrome of acute hemorrhagic hepatitis. YFV transmission involves non-human primates (NHP), mosquitoes and humans. By late 2016, Brazil experienced the largest outbreak last 100 years, with 2050 human confirmed cases, 681 cases ending in death 764 epizootic NHP. Among affected areas, Bahia state Northeastern was only region no autochthonous cases. using next generation sequence approach, we investigated molecular epidemiology NHP discuss what...