Irina Nastassja Riediger
A5001879334- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- Viral gastroenteritis research and epidemiology
- Leptospirosis research and findings
- Influenza Virus Research Studies
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Zoonotic diseases and public health
- Mycobacterium research and diagnosis
- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Infectious Encephalopathies and Encephalitis
- COVID-19 epidemiological studies
- Tuberculosis Research and Epidemiology
- COVID-19 Clinical Research Studies
- HIV/AIDS drug development and treatment
- Microbial infections and disease research
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
Secretaria da Saúde
2019-2023
Universidade Estadual do Paraná
2016-2022
Centro Universitário Moura Lacerda
2010-2021
Fundação Oswaldo Cruz
2017-2021
Universidade Federal da Paraíba
2021
Texas A&M University
2021
Universidade Federal do Rio Grande do Norte
2021
Instituto de Saúde
2021
Ministério da Saúde
2017
Universidade Federal do Paraná
2010
Abstract The high numbers of COVID-19 cases and deaths in Brazil have made Latin America an epicentre the pandemic. SARS-CoV-2 established sustained transmission early pandemic, but important gaps remain our understanding virus dynamics at a national scale. We use 17,135 near-complete genomes sampled from 27 Brazilian states bordering country Paraguay. From March to November 2020, we detected co-circulation multiple viral lineages that were linked importations (predominantly Europe). After...
We characterized 3 autochthonous dengue virus serotype cases and 1 imported case from 2 states in the North South Regions of Brazil, 15 years after Brazil's last outbreak involving this serotype. also identified a new Asian lineage recently introduced into Americas, raising concerns about future outbreaks.
A previous study demonstrates that most of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Brazilian strains fell in three local clades were introduced from Europe around late February 2020. Here we investigated more detail the origin major and widely disseminated SARS-CoV-2 lineage B.1.1.33. We recovered 190 whole viral genomes collected 13 states 29 to April 31, 2020 combined them with other B.1.1 globally. Our genomic survey confirms B.1.1.33 is responsible for a variable...
Abstract Respiratory viral infection can cause severe disease and hospitalization, especially among children, the elderly, patients with comorbidities. In Brazil, official surveillance system of acute respiratory (SARI) investigates influenza A (IAV) B (IBV) viruses, syncytial virus (RSV), adenovirus (HAdV), parainfluenza viruses (hPIV 1–3). Rio Grande do Sul (RS), many fatalities associated SARI between 2013 2017 occurred without underlying diseases for whom causative agent had not been...
A 37-year-old healthcare worker from the northeastern region of Brazil experienced 2 clinical episodes coronavirus disease. Infection with severe acute respiratory syndrome was confirmed by reverse transcription PCR in samples collected 116 days apart. Whole-genome sequencing revealed that infections were caused most prevalent lineage Brazil, B.1.1.33, and emerging P.2. The first infection occurred June 2020; Bayesian analysis suggests reinfection at some point during September 14-October...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemic in Brazil was dominated by two lineages designated as B.1.1.28 and B.1.1.33. SARS-CoV-2 variants harboring mutations at the receptor-binding domain of Spike (S) protein, P.1 P.2, evolved from lineage are rapidly spreading Brazil. Lineage is considered a Variant Concern (VOC) because presence multiple S protein (including K417T, E484K, N501Y), while P.2 only harbors mutation S:E484K Interest (VOI). On other hand,...
Despite the considerable morbidity and mortality of yellow fever virus (YFV) infections in Brazil, our understanding disease outbreaks is hampered by limited viral genomic data. Here, through a combination phylogenetic epidemiological models, we reconstructed recent transmission history YFV within different epidemic seasons Brazil. A suitability index based on highly domesticated Aedes aegypti was able to capture seasonality reported human infections. Spatial modeling revealed spatial...
Mutations at both the receptor-binding domain (RBD) and amino (N)-terminal (NTD) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike (S) glycoprotein can alter its antigenicity promote immune escape. We identified that SARS-CoV-2 lineages circulating in Brazil with mutations concern RBD independently acquired convergent deletions insertions NTD S protein, which altered antigenic-supersite other predicted epitopes this region. Importantly, we detected community transmission...
One of the most remarkable severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern (VOC) features is significant number mutations they acquired. However, specific factors that drove emergence such since second half 2020 are not fully resolved. In this study, we describe a new SARS-CoV-2 P.1 sub-lineage circulating in Brazil, denoted here as Gamma-like-II, well previously described lineage Gamma-like-I shares several lineage-defining with VOC Gamma. Reconstructions...
The COVID-19 epidemic in Brazil experienced two major lineage replacements until mid-2021. first was driven by P.2, late 2020, and the second Gamma, early 2021. To understand how these SARS-CoV-2 lineages spread Brazil, we analyzed 11,724 genomes collected throughout country between September 2020 April Our findings indicate that P.2 probably emerged July Rio de Janeiro state Gamma November Amazonas state. Both states were main hubs of viral disseminations to other Brazilian locations. We...
Abstract The emergence and reemergence of mosquito-borne diseases in Brazil such as yellow fever, zika, chikungunya, dengue have had serious impacts on public health. Concerns been raised due to the rapid dissemination chikungunya virus across country since its first detection 2014 Northeast Brazil. In this work, we carried out on-site training activities genomic surveillance partnership with National Network Public Health Laboratories that led generation 422 genomes from 12 Brazilian states...
Background With a conservatively estimated 1 million cases of leptospirosis worldwide and 5–10% fatality rate, the rapid diagnosis leading to effective clinical public health decision making is high importance, yet remains challenge. Methodology Based on parallel, population-based studies in two leptospirosis-endemic regions Brazil, real-time PCR assay which detects lipL32, gene specifically present pathogenic Leptospira, was assessed for diagnostic effectiveness accuracy. Patients...
The SARS-CoV-2 variant of concern (VOC) Delta was first detected in India October 2020. imported cases the Brazil were identified April 2021 southern region, followed by more different regions during following months. By early September 2021, already dominant southeastern (87%), (73%), and northeastern (52%) Brazilian regions. This study aimed to understand spatiotemporal dissemination dynamics Brazil. To this end, we employed a combination maximum likelihood (ML) Bayesian methods...
Aseptic meningitis is a common viral infection associated with human enteroviruses. The aim of the present study was to identify and characterize enteroviruses outbreaks sporadic cases aseptic that occurred in different regions Brazil between 2013 2017. Cerebrospinal fluids obtained from patients admitted public health facilities were analyzed. A total 303 positive for Human Enteroviruses (EV) by cell culture isolation median rate throughout year 12%. We able enterovirus serotypes 295...
ABSTRACT The Gamma and Delta variants of concern (VOCs) SARS‐CoV‐2 drove the second third wave in Brazil significantly intensified number cases deaths. In this study, we investigate timeline origins using a spatiotemporal analysis based on 1508 genomes collected between March September 2021 from health administrative regions Paraná state, Brazil. Our findings indicate that community transmission Gamma‐P.1 began late 2020, with substantial contributions Northeast North regions. contrast, our...
Leptospirosis is a zoonotic disease usually acquired by contact with water contaminated urine of infected animals. However, few molecular methods have been used to monitor or quantify pathogenic Leptospira in environmental samples. Here we optimized DNA extraction method for the quantification leptospires using previously described Taqman-based qPCR targeting lipL32, gene unique and highly conserved Leptospira. QIAamp mini, MO BIO PowerWater PowerSoil Isolation kits were evaluated extract...
We report the characterization of a novel reassortant influenza A(H1N2) virus not previously reported in humans. Recovered from pig farm worker southeast Brazil who had influenza-like illness, this is triple containing gene segments subtypes H1N2 (hemagglutinin), H3N2 (neuraminidase), and pandemic H1N1 (remaining genes).
Background Hepatitis C virus (HCV) genotyping is the most significant predictor of response to antiviral therapy. The aim this study was develop and evaluate a novel real-time PCR method for HCV based on NS5B region. Methodology/Principal Findings Two triplex reaction sets were designed, one detect genotypes 1a, 1b 3a; another 2a, 2b, 2c. This approach had an overall sensitivity 97.0%, detecting 295 304 tested samples. All samples genotyped by same type that assigned using LiPA version 1...
One hundred thirty-one cases of postsurgical infections were reported in Southern Region Brazil between August 2007 and January 2008. Thirty-nine (29.8%) studied; this report describes epidemiological findings, species identification, antimicrobial susceptibility clonal diversity rapidly growing mycobacteria isolated outbreak.All 39 isolates analyzed by Ziehl-Nielsen stained smear, bacterial culture submitted to rpoB partial gene sequencing for identification. The also evaluated their...
Tuberculosis is a chronic infection caused by strains of the Mycobacterium tuberculosis complex and occurs in both animal human populations. The death tapir showing purulent material hard mass lungs at necropsy raised suspicion potential disease mycobacteria species Brazilian zoo. Later, two other tapirs with similar signs died were further investigated. Polymerase chain reaction (PCR) from bronco-alveolar lavages was performed, animals tested positive for RD(Rio) strain M. tuberculosis,...
Abstract Background Genotyping of hepatitis C virus (HCV) has become an essential tool for prognosis and prediction treatment duration. The aim this study was to compare two HCV genotyping methods: reverse hybridization line probe assay (LiPA v.1) partial sequencing the NS5B region. Methods Plasma 171 patients with chronic were screened using both a commercial method Versant, Siemens, Tarrytown, NY, USA) different primers targeting region PCR amplification analysis. Results Comparison...
Abstract Mutations at both the receptor-binding domain (RBD) and amino (N)-terminal (NTD) of SARS-CoV-2 Spike (S) glycoprotein can alter its antigenicity promote immune escape. We identified that lineages circulating in Brazil with mutations concern RBD independently acquired convergent deletions insertions NTD S protein, which altered antigenic-supersite other predicted epitopes this region. Importantly, we detected communitary transmission four bearing indels: a P.1 Δ69-70 lineage (which...
Abstract One of the most remarkable features SARS-CoV-2 Variants Concern (VOC) is unusually large number mutations they carry. However, specific factors that drove emergence such variants since second half 2020 are not fully resolved. In this study, we described a new lineage provisionally designated as P.1-like-II that, well previously P.1-like-I, shares several lineage-defining with VOC P.1 circulating in Brazil. Reconstructions ancestor sequences demonstrate entire constellation define...