A5000173153- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Cervical Cancer and HPV Research
- Herpesvirus Infections and Treatments
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Virology and Viral Diseases
- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Women's cancer prevention and management
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Reproductive tract infections research
- Animal Virus Infections Studies
- Adolescent Sexual and Reproductive Health
- COVID-19 Clinical Research Studies
- Genital Health and Disease
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Head and Neck Cancer Studies
- Parvovirus B19 Infection Studies
Instituto Nacional do Câncer
2016-2025
Universidade Federal do Rio de Janeiro
2015-2024
Oncovir (United States)
2017-2023
Instituto Nacional del Cáncer
2023
Universidade Federal do Amazonas
2023
Brazilian Micro and Small Enterprises Support Service
2018
Universidade Federal do Rio Grande
2018
D’Or Institute for Research and Education
2017
Rega Institute for Medical Research
2015
KU Leuven
2015
Several recent studies have converged upon the innate immune DNA cytosine deaminase APOBEC3B (A3B) as a significant source of genomic uracil lesions and mutagenesis in multiple human cancers, including those breast, head/neck, cervix, bladder, lung, ovary, other tissues. A3B is upregulated these tumor types relative to normal tissues, but mechanism unclear. Because also has antiviral activity systems member broader response, we tested hypothesis that papillomavirus (HPV) infection causes...
Background The genetic differences among HIV-1 subtypes may be critical to clinical management and drug resistance surveillance as antiretroviral treatment is expanded regions of the world where diverse non-subtype-B viruses predominate. Methods Findings To assess impact subtype on distribution mutations in protease reverse transcriptase, a binomial response model using explanatory variables was used analyze large compiled dataset sequences. Non-subtype-B sequences from 3,686 persons with...
Objective This study aimed to describe the demographic and clinical characteristics of cancer inpatients with COVID-19 exploring outcomes. Methods A retrospective search in electronic medical records admitted Brazilian National Cancer Institute from April 30, 2020 May 26, granted identification 181 patients confirmed by RT-PCR. Results The mean age was 55.3 years (SD ± 21.1). Comorbidities were present 110 (60.8%) cases. most prevalent solid tumors breast (40 [22.1%]), gastrointestinal (24...
Abstract Numerous factors have been identified to influence susceptibility Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and disease severity. Cancer patients are more prone clinically evolve severe COVID-19 conditions, but the determinants of such a outcome remain largely unknown. We determined full-length SARS-CoV-2 genomic sequences cancer healthcare workers (non-cancer controls) by deep sequencing investigated within-host viral population each infection,...
The major limitation of drug resistance genotyping for human immunodeficiency virus remains the interpretation results. We evaluated concordance in predicting therapy response between four different algorithms (Rega 6.3, HIVDB-08/04, ANRS [07/04], and VGI 8.0). Sequences were gathered through a worldwide effort to establish database non-B subtype sequences, demographic clinical information about patients was gathered. most concordant results found nonnucleoside reverse transcriptase (RT)...
The human cervical microbiome is complex, and its role in health disease has just begun to be elucidated. In this study, 57 swab samples from 19 HIV/HPV co-infected women were analyzed for both virome bacteriome composition. Virome analysis focused on circular DNA viruses through rolling circle amplification followed by next-generation sequencing (NGS). Data assigned virus families genera, HPV types identified. NGS data of bacterial 16S a subset 24 operational taxonomic units classified...
Abstract Human Immunodeficiency Virus-1 (HIV-1) antiviral resistance is a major cause of therapy failure and compromises future treatment options. As consequence, testing the standard care. Because high degree HIV-1 natural variation complex interactions, role mutations in many cases insufficiently understood. We applied probabilistic model, Bayesian networks, to analyze direct influences between protein residues exposure clinical protease sequences from diverse subtypes. can determine...
Most of the current knowledge on antiretroviral (ARV) drug development and resistance is based study subtype B HIV-1, which only accounts for 10% worldwide HIV infections. Cumulative evidence has emerged that different types, groups subtypes harbor distinct biological properties, including response susceptibility to ARV. Recent laboratory clinical data highlighting such disparities are summarized in this review. Variations susceptibility, emergence selection specific mutations, viral...
Infection with drug-resistant human immunodeficiency virus type 1 (HIV-1) has been documented in all countries that have surveyed for it and may result an unfavorable response to therapy. The prevalence characteristics of individuals transmitted resistance antiretroviral drugs scarcely described Brazil. We performed testing prior initiation therapy 400 subjects enrolled from 20 centers 13 Brazilian cities between March September 2007. Genotyping was conducted using PCR-amplified HIV pol...
We assessed mortality, treatment response, and relapse among HIV-infected HIV-uninfected women with cervical cancer in Rio de Janeiro, Brazil.
The cervical microbiota composition and diversity of HIV-positive women in the postpartum period is unknown. Using a high-throughput bacterial 16S rRNA gene sequencing, we identified four community state types (CSTs). CST III (Lactobacillusdominant) IV (IV-A, IV-B.1, IV-B.2; high-diversity) were found 41% 59% samples, respectively. We did not find association any to (six or twelve months), HPV infection cytology (normal lesion). However, five genera associated with lesions (Gardnerella,...
Persistent oncogenic HPV infection is strongly associated with cervical cancer. Studies have suggested a higher prevalence of in women living HTLV-1. This study aimed to determine whether HTLV-1 cervicovaginal and characterize types according risk. Vaginal fluid samples were subjected diagnosis via PCR, positive Sanger sequencing massive sequencing. Papanicolaou smears examined using light microscopy identify cell abnormalities. Among the 155 screened, 79 HTLV-1-infected 76 uninfected. PCR...
Simian foamy virus (SFV) is a retrovirus that infects primates, including American primates (AP), but epidemiological studies are often limited to captive animals. The state of Rio de Janeiro (RJ) home an endemic AP species, Leontopithecus rosalia, and invasive chrysomelas, both endangered. This study assessed the molecular prevalence SFV in these species. Genomic DNA was extracted from 48 oral swab samples L. chrysomelas (Niterói/RJ) 102 rosalia (Silva Jardim/RJ). qPCR performed diagnose...
Patients with cancer face a higher risk of severe complications, including death, when affected by COVID-19. Particularly in low- and middle-income countries, COVID-19 pandemic has placed heavy burden on the public health systems, necessitating adjustments clinical management patients. Numerous factors have been identified to influence susceptibility SARS-CoV-2 infection disease severity, but determinants outcomes remain largely unknown. This study aims characterize cytokine lymphocyte...
HIV subtype C is the most prevalent in world. Despite its recent expansion Brazil, HIV-1C already prevails southernmost state of Rio Grande do Sul. This unique epidemiology has prompted us to characterize that population. Seventy-seven HIV-1–infected subjects attending largest HIV/AIDS clinic had protease and reverse transcriptase (RT) genes their virus subtyped genotyped. When subtype-specific infections were plotted according year diagnosis, prevalence was shown increase over last 18 years...
BackgroundAlthough extensive HIV drug resistance information is available for the first 400 amino acids of its reverse transcriptase, impact antiretroviral treatment in C-terminal domains Pol (thumb, connection and RNase H) poorly understood.Methods FindingsWe wanted to characterize conserved regions RT among HIV-1 group M subtypes CRF. Additionally, we wished identify NRTI-related mutations domains. We sequenced 118 H from clinical viral isolates Brazil, analyzed 510 thumb domain 450...