A5029167470- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Neuroscience and Neuropharmacology Research
- HIV-related health complications and treatments
- Photoreceptor and optogenetics research
- GABA and Rice Research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- HIV/AIDS oral health manifestations
- Pneumocystis jirovecii pneumonia detection and treatment
- Blood Coagulation and Thrombosis Mechanisms
- Lymphoma Diagnosis and Treatment
- Medicinal Plant Extracts Effects
- Renal Transplantation Outcomes and Treatments
- Sinusitis and nasal conditions
- Cardiac electrophysiology and arrhythmias
- Parvovirus B19 Infection Studies
- Ion channel regulation and function
- Reproductive Physiology in Livestock
- T-cell and Retrovirus Studies
- Nicotinic Acetylcholine Receptors Study
Centro Hospitalar de Lisboa Ocidental
2013-2018
Hospital de Egas Moniz
2004-2016
KU Leuven
2013
Universidade Nova de Lisboa
2010-2013
UCLouvain
2013
Rega Institute for Medical Research
2013
University of Coimbra
1999-2001
Universidade Federal de Santa Catarina
1997-1999
Universidade Federal de Minas Gerais
1998
Hospital Infantil Joana de Gusmão
1997
Background The genetic differences among HIV-1 subtypes may be critical to clinical management and drug resistance surveillance as antiretroviral treatment is expanded regions of the world where diverse non-subtype-B viruses predominate. Methods Findings To assess impact subtype on distribution mutations in protease reverse transcriptase, a binomial response model using explanatory variables was used analyze large compiled dataset sequences. Non-subtype-B sequences from 3,686 persons with...
The major limitation of drug resistance genotyping for human immunodeficiency virus remains the interpretation results. We evaluated concordance in predicting therapy response between four different algorithms (Rega 6.3, HIVDB-08/04, ANRS [07/04], and VGI 8.0). Sequences were gathered through a worldwide effort to establish database non-B subtype sequences, demographic clinical information about patients was gathered. most concordant results found nonnucleoside reverse transcriptase (RT)...
To investigate whether and how mutations at position 89 of HIV-1 protease were associated with inhibitor (PI) failure, what is the impact subtype.In a database containing pol nucleotide sequences treatment history, correlation between PI experience codon was determined separately for subtype B several non-B subtypes. A Bayesian network model used to map resistance pathways in which M89I/V involved G. The phenotypic effect PIs also measured.The analysis showed that subtypes C, F G wild-type M...
To compare baseline susceptibility to protease inhibitors among HIV-1 isolates of subtypes C, F, G and CRF02_AG, identify polymorphisms that determine the differences in susceptibility.A total 42 samples drug-naive patients infected with (n=19), CRF02_AG (n = 10), F 6) C 7) were phenotyped genotyped Antivirogram ViroSeq 2.0 genotyping system, respectively. A Bayesian network approach was used for a preliminary analysis collected data dependencies indicated by statistically confirmed.CRF02_AG...
Despite improvements in HIV treatment, the prevalence of multidrug resistance and full class is still reported to be increasing. However, investigate whether current treatment strategies are selecting for resistance, incidence, instead prevalence, more informative. Temporal trends (MDR defined as at most 1 drug fully susceptible) (FCR no this Portugal based on 3394 viral isolates genotyped from 2000 2006 were examined using Rega 6.4.1 interpretation system. From July 2001 there was a...
RegaDB is a free and open source data management analysis environment for infectious diseases. allows clinicians to store, manage analyse patient data, including viral genetic sequences. Moreover, provides researchers with mechanism collect in uniform format offers them canvas make newly developed bioinformatics tools available virologists through user friendly interface.Source code, binaries documentation are on http://rega.kuleuven.be/cev/regadb. written the Java programming language,...
In Mozambique, the evaluation of retention in HIV care and ART programmes is limited. To assess rate predictors attrition (no care) HAART effectiveness HIV-1 infected patients who pay for medication laboratory testing we conducted a multicenter survey HIV-1-infected started during 2002–2006. Cox proportional hazard models were used to risk therapy failure. Overall, 142 from 16 healthcare centers located capital city Maputo followed-up 22.2 months (12.1–46.7). The was 75%, 48% 37% after one,...
In this report, we demonstrate that a fetal mouse skin-derived dendritic cell line produces nitric oxide (NO) in response to the endotoxin [lipopolysaccharide (LPS)] and cytokines [tumor necrosis factor-α (TNF-α) granulocyte-macrophage colony-stimulating factor (GM-CSF)]. Expression of inducible isoform NO synthase (iNOS) was confirmed by immunofluorescence with an antibody against iNOS. The tyrosine kinase inhibitor genistein decreased LPS- GM-CSF-induced nitrite ([Formula: see text])...
The effect of ouabain on the uptake tritiated [3H]GABA and its release in absence Ca(+)+ was studied brain cortex synaptosomes. Ouabain, K(+)-depolarization, induces with half-maximal occurring at a concentration about 7 X 10(-6) M. Parallel measurements effects Na+,K(+)-adenosine triphosphatase activity show that inhibits both mechanisms also occurs same concentration. Although is stimulated by ouabain, it appears inhibition due to direct mechanism, inasmuch as initial velocity process...
Objectives To assess HIV-1 diversity, transmission dynamics and prevalence of transmitted drug resistance (TDR) in Angola, five years after ART scale-up. Methods Population sequencing the pol gene was performed on 139 plasma samples collected 2009 from drug-naive infected individuals living Luanda. subtypes were determined using phylogenetic analysis. Drug mutations identified Calibrated Resistance Tool (CPR). Transmission networks analysis all Angolan sequences present databases....
The advent of new therapeutic approaches targeting env and the search for efficient anti-HIV-1 vaccines make it necessary to identify number recombinant forms using genomic regions that were previously not frequently sequenced. In this study, we have subtyped paired pol sequences from HIV-1 strains infecting 152 patients being clinically followed in Portugal. percentage which found discordant subtypes was 25.7%. When subtype concordant (65.1%), most prevalent B (40.8%), by C (17.8%) G...
Despite a decreasing mortality and morbidity in treated HIV-1 patients, highly active antiretroviral treatment (HAART) can still fail due to the development of drug resistance. Especially, multidrug-resistant viruses pose threat efficient therapy. We studied changing prevalence multidrug resistance (MDR) over time cohort HIV-1-infected patients Portugal. used data 8065 followed from July 2001 up April 2012 22 hospitals located MDR at specific date sampling was defined as no more than one...
This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact success rates. Plasma RNA from 81 GT1 was extracted prior to an in-house nested RT-PCR of region, which followed by Sanger population sequencing. NS5A RASs were present 28.4% (23/81) all GT1-infected with 9.9% (8/81) having Y93C/H mutation. NS5B showed a prevalence 14.8% (12/81) only detected GT1b. Overall 38.3%...
Early diagnosis and treatment reduces HIV-1-related mortality, morbidity size of viral reservoirs in infants infected perinatally. Commercial molecular tests enable the early infection but high cost low sensitivity with dried blood spots (DBS) limit their use sub-Saharan Africa.To develop validate a sensitive cheap qualitative proviral DNA PCR-based assay for infant (EID) HIV-1-exposed using DBS samples.Chelex-based method was used to extract from samples followed by nested PCR primers HIV-1...
The investigation of an outbreak cryptosporidiosis in a hospital day-care centre is reported. Twenty-eight (27%) children and one member the staff were infected. Most cases symptomatic major presenting symptom was watery diarrhoea. Shedding oocysts continued for mean 12 days after diarrhoea had subsided most still excreting when first follow-up sample examined. Intermittent shedding or carrier state not seen any cases. Giardia intestinalis cysts detected 19 children, 7 whom showing mixed...
O ambiente influencia a saúde de variadas maneiras: através da exposição factores risco físicos, químicos e biológicos, alterações relacionadas com o comportamento dos indivíduos em resposta aos mesmos factores. As actividades humanas estão afectar clima, implicações graves para pública. Neste sentido, construção uma política integrada constitui um grandes desafios das sociedades contemporâneas. A presente temática começa fazer parte tanto propostas projectos empresas privadas, como...
Strips of rabbit atrium 2-3 mm wide and 10-12 long were used to study how normal propagating action potentials excite a region where slow responses are the only form electrical activity. One end preparation was bathed in Tyrode's solution. The rest exposed solution with high K+ (12.7 mM) Ba++ (1 (TKBa solution). electrically stimulated activity monitored extra- intracellularly (3 M KCl microelectrodes) as it propagated into TKBa-treated region. We observed that response could be elicited...