A5088036668- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis of β-Lactam Compounds
- Hepatitis C virus research
- HIV-related health complications and treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Mosquito-borne diseases and control
- Advanced Drug Delivery Systems
- Synthesis and Catalytic Reactions
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Blood groups and transfusion
- HIV/AIDS Impact and Responses
- Reproductive tract infections research
- Metal complexes synthesis and properties
- vaccines and immunoinformatics approaches
- Drug Solubulity and Delivery Systems
- Respiratory viral infections research
- Magnetism in coordination complexes
- HIV, Drug Use, Sexual Risk
- HIV/AIDS oral health manifestations
University of Lisbon
2014-2024
University of Minho
2021
Escola Superior de Saúde Egas Moniz
2003-2018
Background The baseline susceptibility of primary HIV-2 to maraviroc (MVC) and other entry inhibitors is currently unknown. Methods 19 isolates obtained from asymptomatic AIDS patients seven HIV-1 clinical the fusion enfuvirtide (ENF) T-1249, coreceptor antagonists AMD3100, TAK-779 MVC, was measured using a TZM-bl cell-based assay. 50% inhibitory concentration (IC 50 ), 90% 90 ) dose–response curve slopes were determined for each drug. Results ENF T-1249 significantly less active on than...
Antiretroviral drug nanocarriers hold great promise for developing anti-human immunodeficiency virus (HIV) rectal microbicides. However, challenges remain, namely, concerning which properties are more suited enhancing colorectal distribution and retention of microbicide compounds. In this work, we developed assessed the in vitro vivo performance poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) as carriers model efavirenz (EFV). We particularly focused on effect noncovalent...
Background Unlike in HIV-1 infection, the majority of HIV-2 patients produce broadly reactive neutralizing antibodies, control viral replication and survive as elite controllers. The identification molecular, structural evolutionary footprints underlying these very distinct immunological clinical outcomes may lead to development new strategies for prevention treatment HIV infection. Methodology/Principal Findings We performed a side-by-side comparison C2, V3 C3 envelope regions from HIV-2....
In Mozambique, the evaluation of retention in HIV care and ART programmes is limited. To assess rate predictors attrition (no care) HAART effectiveness HIV-1 infected patients who pay for medication laboratory testing we conducted a multicenter survey HIV-1-infected started during 2002–2006. Cox proportional hazard models were used to risk therapy failure. Overall, 142 from 16 healthcare centers located capital city Maputo followed-up 22.2 months (12.1–46.7). The was 75%, 48% 37% after one,...
The viral load assays AMPLICOR HIV-1 Monitor Test 1.5, Nuclisens QT, and Quantiplex HIV RNA 3.0 (bDNA) were evaluated for their abilities to quantify human immunodeficiency virus type 1 (HIV-1) in 64 plasma samples from 21 children infected Portugal. with subtypes A1, B, F1, G, BG recombinant virus. v1.5 v3.0 detected all samples, there was a good correlation of results between the two kits. Thirty-eight specimens containing subtype or BG, could not be by QT. We also new Retina assay on that...
To characterize HIV-1 diversity and transmitted drug resistance in persons with access to care treatment Maputo, Mozambique.Samples were collected 2002-2004 from 144 drug-naive patients attending public hospitals private clinics. Plasma viremia, CD4, CD8 cell counts determined for each patient. The Stanford Algorithm was used genotyping on pol sequences. Subtyping done by phylogenetic analysis.Most had high viral load (mean, 5.0 log copies/mL) low CD4 (median, 260 cells/microL). Protease...
Background CRF14_BG isolates, originally found in Spain, are characterized by CXCR4 tropism and rapid disease progression. This study aimed to identify the origin of reconstruct its epidemiological history based on new isolates from Portugal. Methodology/Principal Findings C2V3C3 env gene sequences were obtained 62 samples collected 1993–1998 Portuguese HIV-1 patients. Full-length genomic three Viral subtypes, diversity, divergence rate positive selection investigated phylogenetic analysis....
Structural modulation of previously identified lead spiro-β-lactams with antimicrobial activity was carried out.The main objective this work to synthesize and evaluate the biological novel spiro-lactams based on compounds activity.The target chiral spiro-γ-lactams were synthesized through 1,3-dipolar cycloaddition reaction a diazo-γ-lactam electron-deficient dipolarophiles. In vitro against HIV Plasmodium wide range evaluated. Among these compounds, one derivative good anti-HIV two promising...
The high burden of malaria and HIV/AIDS prevents economic social progress in developing countries. A continuing need exists for development novel drugs treatment regimens both diseases order to address the tolerability long-term safety concerns associated with current options emergence drug resistance. We describe new spiro-β-lactam derivatives potent (nM) activity against HIV Plasmodium no bacteria yeast. best performing molecule series, BSS-730A, inhibited HIV-1 HIV-2 replication an IC50...
The prevalence of transmitted human immunodeficiency virus type 1 drug resistance in Angola 2001 196 untreated patients was investigated. All subtypes were detected, along with unclassifiable and complex recombinant strains. Numerous new polymorphisms identified the reverse transcriptase protease. Two (1.6%) unrelated harbored nucleoside inhibitor- nonnucleoside inhibitor-resistant viruses (mutations: M41L, D67N, M184V, L210W, T215Y or T215F, K103N). Continued surveillance is required for...
Objectives To assess HIV-1 diversity, transmission dynamics and prevalence of transmitted drug resistance (TDR) in Angola, five years after ART scale-up. Methods Population sequencing the pol gene was performed on 139 plasma samples collected 2009 from drug-naive infected individuals living Luanda. subtypes were determined using phylogenetic analysis. Drug mutations identified Calibrated Resistance Tool (CPR). Transmission networks analysis all Angolan sequences present databases....
To investigate which HIV-1 genetic forms are circulating in Angola, we have determined the gag and/or env genotypes of 48 isolates from patients living Cabinda and Luanda provinces. The following subtypes were identified: A1 (18 samples, 38%), C (7, 15%), H (5, 10%), J (3, 6%), G (2, 4%), A2 F1 (1, 2%), D 2%). gene fragment was untypable one sample. Discordant subtype classifications genes found eight (17%) samples. There six different recombination patterns (gag/env): A1/H A1/G C/A2 F1/B...
Abstract Diversity of the founding population Human Immunodeficiency Virus Type 1 (HIV-1) transmissions raises many important biological, clinical, and epidemiological issues. In up to 40% sexual infections, there is clear evidence for multiple variants, which can influence efficacy putative prevention methods, reconstruction epidemiologic histories. To infer who-infected-whom, compute probability alternative transmission scenarios while explicitly taking phylogenetic uncertainty into...
Seronegative HIV-1 infection has important implications for diagnosis and prevention. We describe a case of without seroconversion fast progression to AIDS death.Genetic phylogenetic analysis indicated that the patient was infected with CRF14_BG-like strain selectively transmitted by his seropositive sexual partner. The results suggest massive highly aggressive and/or presence an unidentified immunological deficiency prevented formation HIV-1-specific antibodies.
Abstract Background Differently from HIV-1, HIV-2 disease progression usually takes decades without antiretroviral therapy and the majority of infected individuals survive as elite controllers with normal CD4 + T cell counts low or undetectable plasma viral load. Neutralizing antibodies (Nabs) are thought to play a central role in evolution pathogenesis. However, dynamic Nab response resulting escape during acute infection their impact remain largely unknown. Our objective was characterize...
Angola has an extremely diverse HIV-1 epidemic fueled in part by the frequent interchange of people with Democratic Republic Congo (DRC) and (RC). Characterization strains circulating should help to better understand origin subtypes recombinant forms their transmission dynamics. In this study we characterize first near full-length genomic sequences from infected individuals Angola. Samples were obtained 1993 three patients living Cabinda, Near virus isolates. Maximum likelihood phylogenetic...
The biological properties of sixteen structurally related monoanionic gold (III) bis(dithiolene/ diselenolene) complexes were evaluated. differ in the nature heteroatom connected to atom (AuS for dithiolene, AuSe diselenolene), substituent on nitrogen thiazoline ring (Me, Et, Pr, iPr and Bu), exocyclic or group atoms (O, S, Se, C(CN)2) counter-ion (Ph4P+ Et4N+). anticancer antimicrobial activities all investigated, while anti-HIV activity was evaluated only selected complexes. Most showed...