A5043466157- Head and Neck Cancer Studies
- Cervical Cancer and HPV Research
- CRISPR and Genetic Engineering
- Advanced Radiotherapy Techniques
- Cancer-related Molecular Pathways
- Viral-associated cancers and disorders
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Radiomics and Machine Learning in Medical Imaging
- Lung Cancer Research Studies
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Innovative Teaching Methods
- Head and Neck Surgical Oncology
- Animal and Plant Science Education
- interferon and immune responses
- Insect symbiosis and bacterial influences
- Education and Critical Thinking Development
- Colorectal and Anal Carcinomas
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Educational Leadership and Innovation
- Cancer-related molecular mechanisms research
- Genetics, Bioinformatics, and Biomedical Research
- Biomedical Text Mining and Ontologies
Fox Chase Cancer Center
2023
Immersion (United States)
2023
Princeton University
2021
Princeton Public Schools
2019-2020
University of Wisconsin–Madison
2012-2016
University of Wisconsin Carbone Cancer Center
2012-2013
University of Iowa
2013
Center for Cancer Research
2012
Patients with human papillomavirus (HPV+)-associated head and neck cancer (HNC) show significantly improved survival outcome compared those HPV-negative (HPV-) tumors. Published data examining this difference offers conflicting results to date. We systematically investigated the radiation sensitivity of all available validated HPV+ HNC cell lines a series HPV- using in vitro vivo techniques. HNCs exhibited greater intrinsic (average SF2 HPV-: 0.59 vs. HPV+: 0.22; P < 0.0001), corresponding...
Several recent studies have converged upon the innate immune DNA cytosine deaminase APOBEC3B (A3B) as a significant source of genomic uracil lesions and mutagenesis in multiple human cancers, including those breast, head/neck, cervix, bladder, lung, ovary, other tissues. A3B is upregulated these tumor types relative to normal tissues, but mechanism unclear. Because also has antiviral activity systems member broader response, we tested hypothesis that papillomavirus (HPV) infection causes...
To develop a clinically relevant model system to study head and neck squamous cell carcinoma (HNSCC), we have established characterized direct-from-patient tumorgraft of human papillomavirus (HPV)-positive HPV-negative cancers.Patients with newly diagnosed or recurrent HNSCC were consented for donation tumor specimens. Surgically obtained tissue was implanted subcutaneously into immunodeficient mice. During subsequent passages, both formalin-fixed/paraffin-embedded as well flash-frozen...
The human papillomavirus DNA genome undergoes three distinct stages of replication: establishment, maintenance and amplification. We show that the HPV16 E6 protein is required for as an extrachromosomal, nuclear plasmid in its natural host cell, keratinocyte. Based upon mutational analyses, inactivation p53 by E6, but not necessarily E6-mediated degradation p53, was found to correlate with ability support a plasmid. Inactivation dominant negative rescued E6STOP E6SAT mutant genomes replicate...
<div>Abstract<p><b>Purpose:</b> To develop a clinically relevant model system to study head and neck squamous cell carcinoma (HNSCC), we have established characterized direct-from-patient tumorgraft of human papillomavirus (HPV)–positive HPV-negative cancers.</p><p><b>Experimental Design:</b> Patients with newly diagnosed or recurrent HNSCC were consented for donation tumor specimens. Surgically obtained tissue was implanted subcutaneously into...
<div>Abstract<p><b>Purpose:</b> To develop a clinically relevant model system to study head and neck squamous cell carcinoma (HNSCC), we have established characterized direct-from-patient tumorgraft of human papillomavirus (HPV)–positive HPV-negative cancers.</p><p><b>Experimental Design:</b> Patients with newly diagnosed or recurrent HNSCC were consented for donation tumor specimens. Surgically obtained tissue was implanted subcutaneously into...
<p>PDF file - 384K, Detection of multiple HPV subtypes by degenerate PCR genomic DNA (A) or HPV-16 specific Southern blot (B).</p>
<p>PDF file - 384K, Detection of multiple HPV subtypes by degenerate PCR genomic DNA (A) or HPV-16 specific Southern blot (B).</p>
<div>Abstract<p>Patients with human papillomavirus (HPV+)–associated head and neck cancer (HNC) show significantly improved survival outcome compared those HPV-negative (HPV−) tumors. Published data examining this difference offers conflicting results to date. We systematically investigated the radiation sensitivity of all available validated HPV+ HNC cell lines a series HPV− using <i>in vitro</i> vivo</i> techniques. HNCs exhibited greater intrinsic (average...
<div>Abstract<p>Patients with human papillomavirus (HPV+)–associated head and neck cancer (HNC) show significantly improved survival outcome compared those HPV-negative (HPV−) tumors. Published data examining this difference offers conflicting results to date. We systematically investigated the radiation sensitivity of all available validated HPV+ HNC cell lines a series HPV− using <i>in vitro</i> vivo</i> techniques. HNCs exhibited greater intrinsic (average...
Abstract Purpose: We have developed a primary tumorgraft system of squamous cell carcinoma the head and neck with relevance for investigating mechanisms therapeutic resistance, understanding differential responses to standard therapy in HPV-positive HPV-negative cancers, testing novel therapeutics. To better understand power, limitations our system, we initiated molecular characterization these tumors. Methods: Patients newly diagnosed or recurrent HNSCC were consented donation tumor at time...