A5037124767- Hedgehog Signaling Pathway Studies
- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Renal and related cancers
- Tumors and Oncological Cases
- Force Microscopy Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Genetic and Kidney Cyst Diseases
- Cellular Mechanics and Interactions
- Protein Structure and Dynamics
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Developmental Biology and Gene Regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Genetics, Aging, and Longevity in Model Organisms
- Influenza Virus Research Studies
- S100 Proteins and Annexins
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Chemotherapy-induced cardiotoxicity and mitigation
- Machine Learning and Data Classification
- Advanced Bandit Algorithms Research
- Cancer-related Molecular Pathways
- Insect symbiosis and bacterial influences
- Ubiquitin and proteasome pathways
Fox Chase Cancer Center
2016-2023
Immersion (United States)
2020-2023
Temple University Health System
2016-2023
Loma Linda University
2018-2021
Loma Linda University Medical Center
2018-2021
Scripps MD Anderson Cancer Center
2021
Compassionate Cancer Care
2021
University of Illinois Urbana-Champaign
2006-2012
Urbana University
2011
Clemson University
2003
Abstract We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial with density of 100 colonies/cm 2 were obtained by directly ejecting Escherichia coli ( E. ) onto agar‐coated substrates at rapid arraying speed 880 spots per second. Adjusting the concentration suspensions allowed single colonies viable bacteria to be obtained. In addition, as well gradients constructed desktop printers controlled simple...
Astrocytes, the most abundant type of glial cells in brain, play critical roles supporting neuronal development and brain function. Although astrocytes have been frequently detected tumors, including medulloblastoma (MB), their functions tumorigenesis are not clear. Here, we demonstrate that essential components MB tumor microenvironment. Tumor-associated (TAA) secrete ligand sonic hedgehog (Shh), which is required for maintaining cell proliferation despite absence its primary receptor...
Oseltamivir (Tamiflu) is currently the frontline antiviral drug employed to fight flu virus in infected individuals by inhibiting neuraminidase, a protein responsible for release of newly synthesized virions. However, oseltamivir resistance has become critical problem due rapid mutation virus. Unfortunately, how mutations actually confer not well understood. In this study, we employ molecular dynamics (MD) and steered (SMD) simulations, as graphics processing unit (GPU)-accelerated...
Abstract The intermediate filament protein Nestin serves as a biomarker for stem cells and has been used to identify subsets of cancer stem–like cells. However, the mechanistic contributions pathogenesis are not understood. Here, we report that binds hedgehog pathway transcription factor Gli3 mediate development medulloblastomas subtype. In mouse model system, levels increased progressively during medulloblastoma formation, resulting in enhanced tumor growth. Conversely, loss dramatically...
New mutants of human influenza virus (A/H1N1) exhibit resistance to antiviral drugs. The mechanism whereby they develop insensitivity these medications is, however, not yet completely understood. A crystallographic structure A/H1N1 neuraminidase has been published recently. Using molecular dynamic simulations, it is now possible characterize at the atomic level that underlies loss binding affinity In this study, free-energy perturbation was used evaluate relative free energies Tamiflu and...
Here, we examined the role of leukotrienes, well-known inflammatory mediators, in tumorigenesis hedgehog pathway-associated medulloblastoma, and tested efficacies antagonists leukotriene biosynthesis medulloblastoma treatment.Experimental Design: We levels cells by ELISA. next whether synthesis relied on activation pathway, or presence ligand secreted astrocytes. then investigated mediated hedgehog-induced Nestin expression tumor cells. The functions cell proliferation growth were determined...
Doxorubicin and other anthracycline derivatives inhibit topoisomerase II is an important class of cytotoxic chemotherapy in cancer treatment. The use limited by dose-dependent cardiotoxicity, which may manifest initially as asymptomatic cardiac dysfunction with subsequent progression to congestive heart failure. Despite baseline assessment periodic monitoring function for patients receiving agents, there are unmet needs prediction prevention anthracycline-induced cardiotoxicity (AIC).
Hemolytic anemia in the setting of malignancy is a rare manifestation paraneoplastic syndrome with significant morbidity. Here we discuss case involving metastatic breast cancer presenting severe hemolytic and renal failure secondary to thrombotic microangiopathy malignancy. This discusses workup for anemia, possible role therapeutic plasma exchange this setting, as well current understanding management microangiopathic
We have outlined the approach of visualizing autophagy specifically in epithelial follicle stem cells Drosophila ovary using LysoTracker dye. The advantage this protocol is that it details several techniques, including dissection, immunofluorescence, and western blotting, positively identify changes a very small population cells. One limitations needs to be combined with other genetic manipulations positive markers pathway. For complete on use execution protocol, please refer Singh et al., (2018).
<div>Abstract<p>Astrocytes, the most abundant type of glial cells in brain, play critical roles supporting neuronal development and brain function. Although astrocytes have been frequently detected tumors, including medulloblastoma (MB), their functions tumorigenesis are not clear. Here, we demonstrate that essential components MB tumor microenvironment. Tumor-associated (TAA) secrete ligand sonic hedgehog (Shh), which is required for maintaining cell proliferation despite...
<div>Abstract<p>Astrocytes, the most abundant type of glial cells in brain, play critical roles supporting neuronal development and brain function. Although astrocytes have been frequently detected tumors, including medulloblastoma (MB), their functions tumorigenesis are not clear. Here, we demonstrate that essential components MB tumor microenvironment. Tumor-associated (TAA) secrete ligand sonic hedgehog (Shh), which is required for maintaining cell proliferation despite...
<p>MB cells can not maintain Gli1 expression in vitro</p>
<p>Apoptosis in MB slice culture</p>