A5075329916- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
- Metabolism, Diabetes, and Cancer
- Cellular Mechanics and Interactions
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Growth Hormone and Insulin-like Growth Factors
- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Caveolin-1 and cellular processes
- Cellular transport and secretion
- Cancer-related Molecular Pathways
- Mosquito-borne diseases and control
- X-ray Diffraction in Crystallography
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Protein Tyrosine Phosphatases
- Metal Alloys Wear and Properties
- Biochemical and Structural Characterization
- HER2/EGFR in Cancer Research
- Protease and Inhibitor Mechanisms
- Cytokine Signaling Pathways and Interactions
Jiangmen Central Hospital
2017-2025
Fox Chase Cancer Center
2013-2024
Temple University Health System
2016-2024
Chongqing University
2023
Cangzhou Central Hospital
2021-2022
Delft University of Technology
2016-2020
Ghent University
2020
Guangdong Medical College
2017
Key Laboratory of Guangdong Province
2017
Sun Yat-sen University
2017
Yellow fever virus (YFV), a member of the Flavivirus genus, has plus-sense RNA genome encoding single polyprotein. Viral protein NS3 includes protease and helicase that are essential to replication capping. The 1.8-A crystal structure region YFV residues 187 623. Two familiar domains bind nucleotide in triphosphate pocket without base recognition, providing site for nonspecific hydrolysis nucleoside triphosphates triphosphate. third, C-terminal domain unique is proposed function recognition....
// Dong Ren 1, 2, * , Bihua Lin Xin Zhang 3 Yao Peng 4 Ziyu Ye 1 Yan Ma Yangfang Liang 5 Longbin Cao Xiangyong Li Ronggang Lixia Sun Qiongru Liu Jinhua Wu 6 Keyuan Zhou and Jincheng Zeng Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Bioactive Research for Department Education Province, University, Dongguan, 523808, China 2 Orthopedic Surgery, The First Affiliated Hospital Yat-Sen Guangzhou, 510080, Pathology, Jiangmen Central Hospital, Jiangmen, 529030,...
Cancer cells are characterized by a pathological manifestation of uncontrolled proliferation, which results in tumor formation. Therefore, it is necessary to improve understanding the underlying mechanism cell cycle control. Here, we report that miR‑150 downregulated nasopharyngeal carcinoma tissues and cells. Upregulation suppresses (NPC) proliferation induces G1/S arrest vitro, inhibits tumorigenesis vivo. Conversely, silencing yields opposite effect. Our further demonstrate retards...
Phospholipid phosphatase 4 (PPAPDC1A or PLPP4) has been demonstrated to be involved in the malignant process of many cancers. The purpose this study was investigate clinical significance and biological roles PLPP4 lung carcinoma.PLPP4 expression examined 8 paired carcinoma tissues by real-time PCR 265 immunohistochemistry (IHC). Statistical analysis performed evaluate correlation between clinicopathological features survival patients. In vitro vivo assays were assess carcinoma....
Lung adenocarcinoma (LUAD) is a highly malignant tumor with the highest mortality rate among all cancers. Early diagnosis and prognosis are important factors in treatment. Hepatic leukemia factor (HLF) thought to be closely associated lung cancer metastasis. It downregulated tissues negatively correlated number of metastasis-activating circulating cells (CTCs) peripheral blood patients. In this study, we analyzed data from LUAD samples TCGA found that HLF was significantly upregulated EGFR...
Adaptive immunity depends on lymphocyte adhesion that is mediated by the integrin functional antigen 1 (LFA-1). The small guanosine triphosphatase Rap1 regulates LFA-1 adhesiveness through one of its effectors, Rap1-interacting adapter molecule (RIAM). We show RIAM was recruited to plasma membrane (PM) Ras association (RA) and pleckstrin homology (PH) domains, both which were required for adhesion. N terminus inhibited translocation. In vitro, RA domain bound H-Ras with equal but relatively...
The human laminin receptor (LamR) interacts with many ligands, including laminin, prions, Sindbis virus, and the polyphenol (-)-epigallocatechin-3-gallate (EGCG), has been implicated in a number of diseases. LamR is overexpressed on tumor cells, targeting elicits anti-cancer effects. Here, we report crystal structure LamR, which provides insights into its function should facilitate design novel therapeutics LamR.
Abstract The intermediate filament protein Nestin serves as a biomarker for stem cells and has been used to identify subsets of cancer stem–like cells. However, the mechanistic contributions pathogenesis are not understood. Here, we report that binds hedgehog pathway transcription factor Gli3 mediate development medulloblastomas subtype. In mouse model system, levels increased progressively during medulloblastoma formation, resulting in enhanced tumor growth. Conversely, loss dramatically...
Significance Although efforts have been made to determine the structure of talin and way it interacts with integrins through “head” domain, our work shows now that many previous mechanistic models based on adapter are likely be misleading as they constructed a crystal representing an improperly folded head domain. In this work, we identified problem current model proposed FERM-folded head. By analyzing these structural features in cellular context, involving also kindlin adapter, making...
Integrin-dependent crosstalk between cell-matrix adhesions and cell-cell junctions is critical for controlling endothelial permeability proliferation in cancer inflammatory diseases but remains poorly understood. Here, we investigated how acetylation of the distal NPKY-motif Integrin-β1 influences cell physiology barrier function. Expression an acetylation-mimetic β1-K794Q-GFP mutant led to accumulation immature accompanied by a transcriptomic reprograming cells, involving genes associated...
RAP1-interacting adapter molecule (RIAM) mediates RAP1-induced integrin activation. The RAS-association (RA) segment of the RA-PH module RIAM interacts with GTP-bound RAP1 and phosphoinositol 4,5 bisphosphate but this interaction is inhibited by N-terminal RIAM. Here we report structural basis for autoinhibition an intramolecular between IN region (aa 27–93) module. We solved crystal structure IN-RA-PH to a resolution 2.4-Å. reveals that associates RA thereby suppresses RIAM:RAP1...
ABSTRACT Integrin activation and clustering by talin are early steps of cell adhesion. Membrane-bound head domain kindlin bind to the β integrin cytoplasmic tail, cooperating activate heterodimeric integrin, induces in presence Mn2+. Here we show that kindlin-1 can replace Mn2+ mediate β3 induced head, but not F2–F3 fragment talin. mediated was lost upon deletion flexible loop within F1 subdomain. Further mutagenesis identified hydrophobic acidic motifs responsible for clustering. Modeling,...