A5100396999- Cellular Mechanics and Interactions
- Heavy Metal Exposure and Toxicity
- Redox biology and oxidative stress
- Trace Elements in Health
- Nitric Oxide and Endothelin Effects
- Effects and risks of endocrine disrupting chemicals
- Electron Spin Resonance Studies
- Erythrocyte Function and Pathophysiology
- Mercury impact and mitigation studies
- Aluminum toxicity and tolerance in plants and animals
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- bioluminescence and chemiluminescence research
- Muscle Physiology and Disorders
- Hippo pathway signaling and YAP/TAZ
- Heavy metals in environment
- Medicinal Plants and Neuroprotection
- Ultrasound and Hyperthermia Applications
- 3D Printing in Biomedical Research
- Hemoglobin structure and function
- Advanced MRI Techniques and Applications
- Photoreceptor and optogenetics research
- Electrical and Bioimpedance Tomography
- RNA and protein synthesis mechanisms
- Cardiomyopathy and Myosin Studies
University of Virginia
2004-2024
Nanjing University of Aeronautics and Astronautics
2023-2024
Yangzhou University
2023
Jiangsu Provincial Center for Disease Control and Prevention
2023
State Key Laboratory of Food Science and Technology
2014-2022
Jiangnan University
2012-2022
Northeast Agricultural University
2021-2022
Macau University of Science and Technology
2022
Wenzhou Medical University
2020
Shandong Academy of Sciences
2020
ABSTRACT The heavy metal cadmium (Cd) is an environmental pollutant that causes adverse health effects in humans and animals. Our previous work demonstrated oral administration of probiotics can significantly inhibit Cd absorption the intestines mice, but further evidence needed to gain insights into related protection mode. goal this study was evaluate whether through routes other than binding, with a focus on gut barrier protection. In vitro assay, both intervention therapy treatments...
ABSTRACT This study evaluated the protective effects of Lactobacillus plantarum CCFM8610, a selected probiotic with good cadmium binding capacity, against acute toxicity in mice. Ninety mice were divided into prevention and therapy groups. In groups, CCFM8610 was administered at 10 9 CFU once daily for 7 days, followed by single oral dose chloride 1.8 mg each mouse. same 2 days after an identical exposure. Mice that received neither nor culture or alone served as negative positive controls,...
Our previous study confirmed the ability of Lactobacillus plantarum CCFM8610 to protect against acute cadmium (Cd) toxicity in mice. This was designed evaluate protective effects chronic Cd mice and gain insights into protection mode this strain. Experimental were divided two groups exposed for 8 weeks via drinking water or intraperitoneal injection. Both further four subgroups, control, only, plus CCFM8610. Levels measured feces, liver, kidneys, alterations several biomarkers noted. The...
Abstract Necroptosis, a form of programmed cell death, is characterized by the loss membrane integrity and release intracellular contents, execution which depends on membrane-disrupting activity Mixed Lineage Kinase Domain-Like protein (MLKL) upon its phosphorylation. Here we found myofibers committed MLKL-dependent necroptosis after muscle injury. Either pharmacological inhibition upstream kinase Receptor Interacting Protein Kinases 1 (RIPK1) or genetic ablation MLKL expression in led to...
We describe the synthesis and biological applications of a novel nitrogen‐15‐labeled nitrone spin trap, 5‐ethoxycarbonyl‐5‐methyl‐1‐pyrroline N ‐oxide ([ 15 N]EMPO) for detecting superoxide anion. Superoxide anion generated in xanthine/xanthine oxidase (100 nM min −1 ) NADPH/calcium‐calmodulin/nitric oxide synthase systems was readily detected using EMPO, analog 5,5′‐dimethyl‐1‐pyrroline (DMPO). Unlike DMPO‐superoxide adduct (DMPO–OOH), EMPO (EMPO–OOH) does not spontaneously decay to...
Sodium butyrate (NaB) is a short-chain fatty acid which produced by bacterial fermentation of nondigestible dietary fiber and has been reported to exert anti-tumor effects in many tumors including colorectal cancer (CRC). However, the role thioredoxin-1 (Trx-1) NaB-induced effect not completely clarified.Effects NaB on growth CRC cell lines HT29 SW480 were detected Cell Counting Kit-8 (CCK-8) colony formation assays. The apoptotic cells determined flow cytometry, migration was assessed...
Cadmium is a common environmental pollutant and used for industrial purposes all over the world. exposure causes variety of public health issues, mainly reproductive health. The present review discusses information related to female health, including during after pregnancy its outcomes. alters steroidogenesis, delays puberty, loss hormones, disturbs menstrual cycle, premature births, reduces birth weights in females. Besides that, their offspring are affected differently by prenatal mother...
Cell migration is essential for regulating many biological processes in physiological or pathological conditions, including embryonic development and cancer invasion. In vitro silico studies suggest that collective cell associated with some biomechanical particularities such as restructuring of extracellular matrix (ECM), stress force distribution profiles, reorganization the cytoskeleton. Therefore, phenomenon could be understood by an in-depth study cells' behavior determinants, but not...
We investigated the effects of a cysteine residue on tyrosine nitration in several model peptides treated with myeloperoxidase (MPO), H2O2, and nitrite anion (NO2-) horseradish peroxidase H2O2. Sequences were acetyl-Tyr-Cys-amide (YC), acetyl-Tyr-Ala-Cys-amide (YAC), acetyl-Tyr-Ala-Ala-Cys-amide (YAAC), acetyl-Tyr-Ala-Ala-Ala-Ala-Cys-amide (YAAAAC). Results indicate that oxidation products tyrosyl YC other barely detectable. A major product detected was corresponding disulfide (e.g....
We used steady-state and time-resolved electronic vibrational spectroscopies to reveal a “green” chromophore in “red” protein with single amino substituent, rationally designed aY-sfGFP mutants significantly brighter red emission.
Cadmium (Cd) is a toxic metal inducing range of adverse effects on organs including liver and kidneys. However, the underlying molecular mechanisms Cd-induced intestinal toxicity through dietary intake poorly studied. This study evaluated Cd physiology confirmed effectiveness protective mechanism probiotic Lactobacillus plantarum CCFM8610 against chronic toxicity. After treatment with Cd, HT-29 cell line was subjected to iTRAQ analysis, which revealed that changes in proteomic profiles after...
Vinculin is a talin-binding protein that promotes integrin-mediated cell adhesion, but the mechanisms are not understood. Because talin direct activator of integrins, we asked whether and how vinculin regulates formation integrin: complexes. We report VD1 (aa 1-258) its mutant, VD1A50I, bind directly equally to several β integrin cytoplasmic tails (βCT). Results from competition assays show VD1, inhibits interaction (Tn) rod (TnR), head (TnH) with β3CT. The inhibition observed could be...
Here, we investigated the effect of bicarbonate anion (HCO3-) on peroxidase activity stimulated by thiol oxidase copper, zinc superoxide dismutase (SOD1) using electron spin resonance (ESR) and optical techniques. Low temperature direct ESR revealed that cysteine (Cys) caused reduction copper(II) to copper(I) was reoxidized molecular oxygen at active site SOD1. The addition HCO3- aerobic incubations containing SOD1, Cys, DTPA in phosphate buffer enhanced as measured hydroxylation cyclic...