A5052858229- Telomeres, Telomerase, and Senescence
- Adipose Tissue and Metabolism
- Cervical Cancer and HPV Research
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Skin Protection and Aging
- Virus-based gene therapy research
- Cancer-related Molecular Pathways
- Diabetes and associated disorders
- Head and Neck Cancer Studies
- Toxic Organic Pollutants Impact
- Neuroendocrine Tumor Research Advances
- Immune Response and Inflammation
- DNA Repair Mechanisms
- Cytomegalovirus and herpesvirus research
- Effects and risks of endocrine disrupting chemicals
- NF-κB Signaling Pathways
- Mesenchymal stem cell research
- interferon and immune responses
- Cardiovascular Disease and Adiposity
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Viral Infections and Outbreaks Research
- Wound Healing and Treatments
- Antimicrobial Resistance in Staphylococcus
University of Iowa
2016-2025
Fraternal Order of Eagles
2016-2024
Diabetes Research Center
2024
University of Wisconsin Carbone Cancer Center
2013
University of Wisconsin–Madison
1991-2013
Research Network (United States)
2013
University of Iowa Stead Family Children’s Hospital
2008-2009
Czech Academy of Sciences, Institute of Microbiology
2003-2007
United States Department of Veterans Affairs
2006
University of Iowa Hospitals and Clinics
2006
Patients with human papillomavirus (HPV+)-associated head and neck cancer (HNC) show significantly improved survival outcome compared those HPV-negative (HPV-) tumors. Published data examining this difference offers conflicting results to date. We systematically investigated the radiation sensitivity of all available validated HPV+ HNC cell lines a series HPV- using in vitro vivo techniques. HNCs exhibited greater intrinsic (average SF2 HPV-: 0.59 vs. HPV+: 0.22; P < 0.0001), corresponding...
Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of death in United States, and cigarette smoking major risk factor for COPD. Fibroblasts play an important role repair lung homeostasis. Recent studies have demonstrated a reduced growth rate fibroblasts patients with In this study we examined effect smoke extract (CSE) on fibroblast proliferative capacity. We found that stopped proliferation upregulated two pathways linked to cell senescence (a biological process...
Abstract Adipose tissue dysfunction is critical to the development of type II diabetes and other metabolic diseases. While monolayer cell culture has been useful for studying fat biology, 2D often does not reflect complexity tissue. Animal models are also problematic in that they expensive, time consuming, may completely recapitulate human biology because species variation. To address these problems, we have developed a scaffold-free method generate 3D adipose spheroids from primary or...
Ebola virus (EBOV) causes severe human disease. During late infection, EBOV virions are on the skin’s surface; however, permissive skin cell types and route of translocation to epidermal surface unknown. We describe a explant model demonstrate that infection via basal media increases in time-dependent dose-dependent manner. In dermis, cells myeloid, endothelial, fibroblast origin were antigen–positive whereas keratinocytes harbored epidermis. Infectious was detected apical within 3 days,...
The Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) functions to maintain latency in EBV-infected B lymphocytes. Although LMP2A is nonessential for the immortalization of lymphocytes by EBV, its expression prevents viral reactivation blocking B-cell receptor activation and signaling. also provides an antiapoptotic signal transgenic mice that express activates phosphatidylinositol 3-kinase (PI3K) serine/threonine kinase Akt epithelial cells. Here we show EBV PI3K beta-catenin...
In this study, we utilized the reverse transcriptase component of telomerase, hTERT, and human papillomavirus type 16 (HPV-16) E6 E7 genes to transform normal cystic fibrosis (CF) airway epithelial (HAE) cells. One cell line, designated NuLi-1 (normal lung, University Iowa), was derived from HAE genotype; three lines, CuFi (cystic fibrosis, Iowa)-1, CuFi-3, CuFi-4, were various CF genotypes. When grown at air-liquid interface, lines capable forming polarized differentiated epithelia that...
Loss of telomeres has been hypothesized to be important in cellular senescence and may play a role carcinogenesis. In this study, we have measured telomere length association with the immortalization transformation human cervical foreskin epithelial cells by papillomavirus type 16 or 18 E6 E7 open reading frames. By using telomeric TTAGGG repeat probe, it was shown that precrisis normal E6-, E7-, E6/E7-expressing gradually shortened passaging (30 100 bp per population doubling). Cells...
Infection with human papillomavirus (HPV) is a critical factor in the pathogenesis of most cervical cancers and some aerodigestive cancers. The HPV E6 oncoprotein from high-risk types contributes to immortalization transformation cells by multiple mechanisms, including degradation p53, transcriptional activation telomerase reverse transcriptase (hTERT), several proteins containing PDZ domains. ability bind domain-containing independent p53 or hTERT but does correlate oncogenic potential (R....
The human papillomavirus (HPV) oncogene E6 has been shown to perform multiple functions (p53 degradation, telomerase activation, etc.) that play a role in oncogenic transformation. Beyond known functions, an undefined mechanism allows cellular invasion requires the PDZ binding motif (PDZBM). Here, we show HPV type 16 (HPV16) interacts with and induces loss of protein tyrosine phosphatase (PTPN13) PDZBM-dependent manner. PTPN13 induced either by presence or short hairpin RNA strategy for...
3,3′,4,4′,5-Pentachlorobiphenyl (PCB126), a dioxin-like polychlorinated biphenyl (PCB) and potent aryl hydrocarbon receptor (AhR) agonist, is implicated in the disruption of both carbohydrate lipid metabolism which ultimately leads to wasting disorders, metabolic disease, nonalcoholic fatty liver disease. However, mechanisms are unclear. Because target organ for PCB toxicity responsible homeostasis, we hypothesized that early glucose homeostasis contributes later manifestations such as...
Senescence compromises the ability of chondrocytes to maintain and repair articular cartilage. We hypothesized that oxidative stress telomere loss contribute chondrocyte senescence. To test this hypothesis, we compared growth human cartilage incubated in 5% O2 21% O2. Cells grown reached 60 population doublings (PD) before senescing, but induced DNA damage premature senescence at less than 40 PD. Human telomerase reverse transcriptase (hTERT)-transduction failed prevent O2, allowed 1 3...
Despite knowledge of the effects toxic shock syndrome (TSS) toxin 1 (TSST-1) on adaptive immune system, little is known about stimulation innate particularly epithelial cells. This study investigated interactions TSS Staphylococcus aureus and TSST-1 with human vaginal cells (HVECs) porcine mucosal surfaces. When cocultured HVECs for 6 h, S. MN8 proliferated, formed aggregates HVEC surfaces, produced exotoxins. Receptor binding studies showed that 35S-TSST-1 bound to 5 x 10(4) receptors per...
<h3>Objective</h3> To provide a manipulatable system to study the mechanism of human papillomavirus 16 (HPV16)<i>E6</i>–related transformation an epithelial cell type affected by HPV16 in humans. <h3>Design</h3> Biochemical and physiological studies mouse tonsil cells (MTECs) transformed with<i>HPV16</i>oncogenes plus H<i>-ras</i>in vitro vivo. <h3>Setting</h3> Basic research laboratory. <h3>Participants</h3> C57BL/6 mice. <h3>Interventions</h3> Transduction...
Excessive weight and obesity are associated with the development of diabetes mellitus type 2 (DMII) in humans. They also pose high risks Staphylococcus aureus colonization overt infections. S. causes a wide range severe illnesses both healthy immunocompromised individuals. Among virulence factors, superantigens essential for pathogenicity. In this study, we show that rabbits chronically exposed to superantigen toxic shock syndrome toxin-1 (TSST-1) experience impaired glucose tolerance,...