A5051254377- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Neuroendocrine Tumor Research Advances
- Immune Response and Inflammation
- Viral Infections and Outbreaks Research
- RNA Research and Splicing
- Fatty Acid Research and Health
- Toxic Organic Pollutants Impact
- Telomeres, Telomerase, and Senescence
- interferon and immune responses
- RNA modifications and cancer
- Nuclear Structure and Function
- Influenza Virus Research Studies
- Pancreatic and Hepatic Oncology Research
- Enzyme Production and Characterization
- Wound Healing and Treatments
- Receptor Mechanisms and Signaling
- Neuroblastoma Research and Treatments
- Fibroblast Growth Factor Research
- Streptococcal Infections and Treatments
- Microbial Metabolites in Food Biotechnology
- Renin-Angiotensin System Studies
- NF-κB Signaling Pathways
University of Iowa
2016-2025
Carver Bible College
2024
University of Iowa Hospitals and Clinics
2008
University of New Mexico
2007
Université de Reims Champagne-Ardenne
1999
Abstract Adipose tissue dysfunction is critical to the development of type II diabetes and other metabolic diseases. While monolayer cell culture has been useful for studying fat biology, 2D often does not reflect complexity tissue. Animal models are also problematic in that they expensive, time consuming, may completely recapitulate human biology because species variation. To address these problems, we have developed a scaffold-free method generate 3D adipose spheroids from primary or...
Ebola virus (EBOV) causes severe human disease. During late infection, EBOV virions are on the skin’s surface; however, permissive skin cell types and route of translocation to epidermal surface unknown. We describe a explant model demonstrate that infection via basal media increases in time-dependent dose-dependent manner. In dermis, cells myeloid, endothelial, fibroblast origin were antigen–positive whereas keratinocytes harbored epidermis. Infectious was detected apical within 3 days,...
Summary Dyskeratosis congenita (DC), an inherited bone marrow failure syndrome, is caused by defects in telomerase. Somatic cells from DC patients have shortened telomeres and clinical symptoms are most pronounced organs with a high cell turnover, including those involved hematopoiesis skin function. We previously identified autosomal dominant (AD) form of that mutations the telomerase RNA component (TER). In this study, we evaluated whether retroviral expression TER and/or reverse...
Activation of the brain renin-angiotensin system (RAS) stimulates energy expenditure through increasing resting metabolic rate (RMR), and this effect requires simultaneous suppression circulating and/or adipose RAS. To identify mechanism by which peripheral RAS opposes RMR control RAS, we examined mice with transgenic activation (sRA mice). sRA exhibit increased flux in inguinal tissue, is attenuated angiotensin II type 2 receptor (AT2) activation. AT2 adipocytes norepinephrine-induced...
The omentum is the primary site of metastasis for ovarian cancer (OC). Interactions between cells and adipocytes drive an invasive pro-metastatic phenotype. Here we studied cell-adipocyte crosstalk by using a direct co-culture model with immortalized human visceral pre-adipocytes (VNPAD) OC cells. We demonstrate increased proliferation, invasiveness, resistance to cisplatin co-cultured compared mono-cultured RNA-sequencing from vs. mono-culture revealed significant transcriptomic changes,...
Ebola virus (EBOV), the causative agent of disease (EVD), remains one WHO's top ten threats to global health. Infectious EBOV virions can be found on surface skin late during systemic infection and passed from deceased through skin-to-skin contact. Here, we assess viral load antigen expression in EBOV-infected non-human primates (NHP) mouse adapted-EBOV (ma-EBOV) -infected mice use low containment model, rVSV/EBOV GP, mechanistically define mice. Viral RNA peaked within proximal site...
Background Human adipocytes may have significant functions in wound healing and the development of diabetes through production pro-inflammatory cytokines after stimulation by gram-negative bacterial endotoxin. Diabetic foot ulcers are most often associated with staphylococcal infections. Adipocyte responses area play a role persistence pathology. We studied effect superantigens (SAgs) on immortalized human adipocytes, alone presence endotoxin or α-toxin. Methodology/Principal Findings...
Mechanisms of neuroendocrine tumor (NET) proliferation are poorly understood, and therapies that effectively control NET progression metastatic disease limited. We found amplification a putative oncogene, RABL6A, in primary human pancreatic NETs (PNET) correlated with high-level RABL6A protein expression. Consistent those results, stable silencing cultured BON-1 PNET cells revealed it is essential for their survival. Cells lacking predominantly arrested G1 phase moderate mitotic block....
Menstrual toxic shock syndrome (TSS) is a serious infectious disease associated with vaginal colonization by Staphylococcus aureus producing the exotoxin TSS toxin 1 (TSST-1). We show that menstrual occurs after TSST-1 interaction an immune costimulatory molecule called CD40 on surface of epithelial cells. Other related toxins, where entire family superantigen family, bind to CD40, but not high-enough apparent affinity cause TSS; thus, only associated. Once cells become activated TSST-1,...
Chondrocytes are mesenchymally derived cells that reportedly acquire some epithelial characteristics; however, whether this is a progression through mesenchymal to transition (MET) during chondrosarcoma development still matter of investigation. We observed acquired the expression four markers, E-cadherin,desmocollin 3, maspin, and 14-3-3σ, all which governed epigenetically cytosine methylation. Indeed, loss methylation was tightly associated with both maspin 14-3-3σ in chondrosarcomas. In...
Chlamydia trachomatis serovars A-C infect conjunctival epithelial cells and untreated infection can lead to blindness. D-K genital tract resulting in pelvic inflammatory disease, ectopic pregnancy, sterility while L1-L3 macrophages, causing an invasive infection. Despite some strains of chlamydia sharing high nucleotide sequence similarity, the bacterial host factors that govern tissue cellular tropism remain largely unknown. Following introduction C.trachomatis via intercourse, vagina,...
Dyskeratosis congenita (DC) is characterized by the triad of reticulate skin pigmentation, nail dystrophy and leukoplakia. Epidermal atrophy, hair growth defects, bone marrow failure increased risk cancer are also common in DC patients. caused mutations genes encoding for telomerase complex factors. Although there an association epidermal abnormalities with DC, cells from donors have not been previously characterized. We isolated keratinocytes affected members a family autosomal dominant...
Abstract The node, or its zebrafish equivalent, Kupffers Vesicle (KV), is thought to generate laterality cues through cilia‐dependent signaling. An interaction between Nodal ligands and antagonists around the node/KV also required. Here we investigate whether loss of Brachyury/Notail Tbx16/Spadetail disrupts balance (Southpaw) (Charon) Vesicle. Reduction Spadetail Notail expression southpaw in perinodal domains flanking Similar what was published for Notail, find required charon . We present...
ABSTRACT Staphylococcus aureus infective endocarditis (IE) is a fast-progressing and tissue-destructive infection of the cardiac endothelium. The superantigens (SAgs) toxic shock syndrome toxin 1 (TSST-1), staphylococcal enterotoxin C (SEC), toxins encoded by gene cluster ( egc ) play novel essential role in etiology S. IE. Recent studies indicate that SAgs act at site to cause tissue pathology promote vegetation growth. underlying mechanism SAg involvement has not been clearly defined. In...
Abstract Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute the wound healing process. However, details of how these interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we whether adipocytes precursors (pre-adipocytes) secrete factors that affect dermal fibroblast behavior. We found both adipocyte pre-adipocyte conditioned medium...
Aryl hydrocarbon receptor (AHR) agonists such as dioxin have been associated with obesity and the development of diabetes. Whole-body Ahr knockout mice on high-fat diet (HFD) shown to resist hepatic steatosis. Tissue-specific in mature adipocytes via adiponectin-Cre exacerbates while liver increases steatosis without having significant effects obesity. Our previous studies demonstrated that treatment subcutaneous preadipocytes exogenous or endogenous AHR disrupts maturation into functional...